FIGURE 2
FROM:
Environmental signal integration by a modular AND gate
J Christopher Anderson, Christopher A Voigt & Adam P Arkin
doi:10.1038/msb4100173
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Integration of two inducible promoters by the AND gate. (A) The fluorescence was measured for 64 combinations of inducer in a fluorimeter. The data are shown for (left to right) 0, 3.2
10-7, 1.3
10-6, 5.2
10-6, 2.1
10-5, 8.3
10-5, 3.3
10-4, and 1.3
10-3 M arabinose, and (bottom to top) 0, 1.5
10-7, 6.1
10-7, 2.4
10-6, 9.8
10-6, 3.9
10-5, 1.6
10-4, and 6.2
10-4 M salicylate. (B) The fluorescence was measured in individual cells using a flow cytometer to determine the population level behavior. The entire population of cells is turned on in the presence of both arabinose and salicylate (1.3
10-3 and 6.2
10-4 M, respectively). When either inducer is not added, the entire population is turned off. There is a 1000-fold induction between the ON and OFF states. The data for this figure were obtained using plasmids pAC-SalSer914, pBACr-AraT7940, and pBR939b (Supplementary information).
