FIGURE 1
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Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection
Tomoya Baba, Takeshi Ara, Miki Hasegawa, Yuki Takai, Yoshiko Okumura, Miki Baba, Kirill A Datsenko, Masaru Tomita, Barry L Wanner & Hirotada Mori
doi:10.1038/msb4100050
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Derivation of E. coli K-12 BW25113. Strain BD792, like MG1655, is a two-step descendent of ancestral E. coli K-12, EMG2, originally called WG1 (Bachmann, 1996; Hayashi et al, 2006; late BJ Bachmann, personal communication). Like its predecessor W1485F+ (Hayashi et al, 2006), BD792 has the rpoS396(Am) allele (codon 33, TAG (Am); unpublished data). Strain BW25113 was derived from BD792 in a series of steps involving generalized transduction and allele replacements, which included introducing the pseudoreversion rpoS (Q33) allele from MG1655 into a predecessor of BW25113 (Supplementary Table 1). The derivation of W3110 is shown in Figure 1 of accompanying manuscript (Hayashi et al, 2006).
