FIGURE 3 

Cutting parts from T7.1. (A) Restriction enzymes specific to the sites that bracket parts (P#.Enzyme) and added unique restriction sites (U#.Enzyme) were used to cut section alpha (Supplementary information). A subset of the digests is shown. As built, part 1 cannot be removed. (B) Restriction digests cutting out all parts in section beta. As built, part 28 cannot be removed.