FIGURE 5
FROM:
Role of protein kinase C
in the regulated secretion of the amyloid precursor protein
M Racchi, M Mazzucchelli, A Pascale, M Sironi and S Govoni
BACK TO ARTICLEFigure 5.
Carbachol-mediated sAPP
release is partially dependent on MAP-kinase activation. Cells (SYwt and SY4) were preincubated overnight with serum-free MEM and then treated for 10 min with 1 mM carbachol following 30 min pretreatment with vehicle or 50 
M PD98059. For sAPP release (panel (b)), the cells were incubated in the presence or absence of carbachol for 2 h. Cell lysates and conditioned media were collected and treated as indicated in the 'Materials and methods' section. As indicated in panel (a), pretreatment with PD98059 effectively blocks ERKs phosphorylation, while only partially blocking (panel (b)) the effect of carbachol on sAPP release.
