FIGURE 4
FROM:
Role of protein kinase C
in the regulated secretion of the amyloid precursor protein
M Racchi, M Mazzucchelli, A Pascale, M Sironi and S Govoni
BACK TO ARTICLEFigure 4.
Carbachol promotes the non-amyloidogenic metabolism of APP similarly in wild-type cells and in antisense transfected neuroblastoma cells. Cells were incubated for 2 h with serum-free medium alone or increasing concentrations of CCh (10 
M, 100 M, 1 mM). Proteins released into the conditioned media were collected and subjected to Western blot analysis for sAPP
. The blots were subsequently quantified by densitometry as described in the 'Materials and methods' section. Data are expressed as a percentage of basal release and are representative of three to four independent experiments.
