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| Immediate communication |
| High throughput fluorescent CE-SSCP SNP genotyping |
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| D Gonen1, J Veenstra-VanderWeele1, Z Yang1, B L Leventhal1,2 and E H Cook Jr1,2 |
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1Laboratory for Developmental Neuroscience, Child and Adolescent Psychiatry, Department of Psychiatry, University of Chicago, MC3077, 5841 S Maryland Avenue, Chicago, Illinois 60637, USA
2Department of Pediatrics, University of Chicago, MC3077, 5841 S Maryland Avenue, Chicago, Illinois 60637, USA
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Correspondence to: EH Cook Jr MD, Department of Psychiatry MC3077, University of Chicago, 5841 South Maryland Avenue, Chicago, IL 60637, USA. E-mail: ed@yoda.bsd.uchicago.edu
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| Abstract |
| Large numbers of single nucleotide polymorphisms (SNPs) are being identified by several laboratories for the purpose of developing dense genetic maps. Single-strand conformation polymorphism (SSCP) analysis has been widely used as a method for detecting novel sequence variations in PCR products. Differences in migration of single-stranded DNA can be used not only to find mutations, but to genotype SNPs in large sample populations. Using PCR with fluorescent labeling and automated capillary electrophoresis SSCP (CE-SSCP), we have developed a panel of 15 functional candidate SNPs. With an automated single capillary instrument, relatively rapid and low cost CE-SSCP SNP genotyping using currently available technology is feasible for 135 000 genotypes per year. With parallel multiple array capillary electrophoresis, more genotypes per year may be attainable. |
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| Keywords |
| genotyping; capillary electrophoresis; SNP; polymorphism |
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| Received 25 March 1999; accepted 28 April 1999 |
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| July 1999, Volume 4, Number 4, Pages 339-343 |
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