Original Article

Molecular Psychiatry (2017) 22, 724–732; doi:10.1038/mp.2016.132 published online 30 August 2016

Heightened extended amygdala metabolism following threat characterizes the early phenotypic risk to develop anxiety-related psychopathology

A J Shackman1,2,3,12, A S Fox4,12, J A Oler5,6,7, S E Shelton5, T R Oakes8, R J Davidson5,6,9,10,11 and N H Kalin5,6,7,11

  1. 1Department of Psychology, University of Maryland, College Park, MD, USA
  2. 2Neuroscience and Cognitive Science Program, University of Maryland, College Park, MD, USA
  3. 3Maryland Neuroimaging Center, University of Maryland, College Park, MD, USA
  4. 4Department of Psychology and California National Primate Research Center, University of California, Davis, CA, USA
  5. 5Department of Psychiatry, University of Wisconsin, Madison, WI, USA
  6. 6HealthEmotions Research Institute, University of Wisconsin, Madison, WI, USA
  7. 7Lane Neuroimaging Laboratory, University of Wisconsin, Madison, WI, USA
  8. 8inseRT MRI, Inc., Middleton, WI, USA
  9. 9Department of Psychology, University of Wisconsin, Madison, WI, USA
  10. 10Center for Investigating Healthy Minds, University of Wisconsin, Madison, WI, USA
  11. 11Waisman Laboratory for Brain Imaging and Behavior, University of Wisconsin, Madison, WI, USA

Correspondence: Dr NH Kalin, Department of Psychiatry, University of Wisconsin, 6001 Research Park Boulevard, Madison, Wisconsin 53719, USA. E-mail: nkalin@wisc.edu

12These authors contributed equally to this work.

Received 31 October 2015; Revised 19 May 2016; Accepted 1 June 2016
Advance online publication 30 August 2016

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Abstract

Children with an anxious temperament are prone to heightened shyness and behavioral inhibition (BI). When chronic and extreme, this anxious, inhibited phenotype is an important early-life risk factor for the development of anxiety disorders, depression and co-morbid substance abuse. Individuals with extreme anxious temperament often show persistent distress in the absence of immediate threat and this contextually inappropriate anxiety predicts future symptom development. Despite its clear clinical relevance, the neural circuitry governing the maladaptive persistence of anxiety remains unclear. Here, we used a well-established nonhuman primate model of childhood temperament and high-resolution 18fluorodeoxyglucose positron emission tomography (FDG-PET) imaging to understand the neural systems governing persistent anxiety and to clarify their relevance to early-life phenotypic risk. We focused on BI, a core component of anxious temperament, because it affords the moment-by-moment temporal resolution needed to assess contextually appropriate and inappropriate anxiety. From a pool of 109 peri-adolescent rhesus monkeys, we formed groups characterized by high or low levels of BI, as indexed by freezing in response to an unfamiliar human intruder’s profile. The high-BI group showed consistently elevated signs of anxiety and wariness across >2 years of assessments. At the time of brain imaging, 1.5 years after initial phenotyping, the high-BI group showed persistently elevated freezing during a 30-min ‘recovery’ period following an encounter with the intruder—more than an order of magnitude greater than the low-BI group—and this was associated with increased metabolism in the bed nucleus of the stria terminalis, a key component of the central extended amygdala. These observations provide a neurobiological framework for understanding the early phenotypic risk to develop anxiety-related psychopathology, for accelerating the development of improved interventions, and for understanding the origins of childhood temperament.