Abstract
Ductal carcinoma in situ (DCIS) is a heterogeneous, unicentric precursor of invasive breast cancer, which is frequently identified through mammographic breast screening programs. The lesion can cause particular difficulties for specimen handling in the laboratory and typically requires even more diligent macroscopic assessment and sampling than invasive disease. Pitfalls and tips for macroscopic handling, microscopic diagnosis and assessment, including determination of prognostic factors, such as cytonuclear grade, presence or absence of necrosis, size of the lesion and distance to margins are described. All should be routinely included in histopathology reports of this disease; in order not to omit these clinically relevant details, synoptic reports, such as that produced by the College of American Pathologists are recommended. No biomarkers have been convincingly shown, and validated, to predict the behavior of DCIS till date.
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Main
Ductal carcinoma in situ (DCIS) is a malignant, clonal proliferation of cells growing within the basement membrane-bound structures of the breast and with no evidence of invasion into surrounding stroma. Where mammographic breast screening is not performed, DCIS constitutes approximately 5% of breast cancers but within screening programs it comprises approximately 20–25% of these tumors. However, it is now generally accepted that DCIS is not one entity but rather a heterogeneous group of lesions clinically, radiologically, morphologically and genetically. This heterogeneity adds complexity to the pathologist's role, in macroscopic specimen handling, and microscopic diagnosis and prognostic feature assessment.
Specimen handling
This clinical treatment of DCIS is surgical excision with clear margins.1 For large lesions this may require mastectomy but for smaller, screen-detected lesions, a good cosmetic result can be obtained by wide local excision. Recurrence of DCIS generally occurs at the site of previous excision and it is therefore better regarded as residual disease, as demonstrated by studies showing concordance of genetic pattern with comparative genomic hybridization (CGH) in primary and recurrent lesions.2 In addition, approximately half of the recurrences after breast-conserving surgical excision for DCIS are as invasive disease,3 with potential to spread outside of the breast. Thus, complete surgical excision with accurate pathological assessment and reporting is essential to optimize treatment of a curable in situ process that may otherwise progress into, or recur as, invasive carcinoma.
Although clear margins of excision of DCIS are clearly important prognostically, with regard to local recurrence of disease,4 there is, no widely agreed margin width of clearance at which the DCIS is deemed ‘completely excised’. If DCIS is excised by 1 mm or less, local recurrence is more common, as is residual disease in a subsequent re-excision specimen or completion mastectomy.5, 6, 7, 8 However, a range of minimum width of surrounding uninvolved tissue is recommended in different centers, from 2 (with radiotherapy) to 10 mm or more being regarded as adequate clearance. This lack of a uniform definition for ‘complete’ excision has implications with regard to handling of the surgical excision specimen within the laboratory.
Ductal carcinoma in situ is most commonly surgically excised on the basis of the placement of needle localization wires by the radiologist, who may bracket the lesion with more than one wire. Thus, the specimen is received bearing one or more wires in the histology laboratory. It should be weighed and measured. The surgeon should orientate the specimen with clips or sutures according to local protocol and different margins can then be painted with marker inks. These allow identification of the different margins histologically (Figure 1), such that if disease does extend to one margin, this aspect alone can be re-excised, without resorting to excision of the entire cavity. On subsequent slicing, either in the fresh state or after fixation, there may be prominent fibrous tissue around the ducts or, small specks of necrotic material may extrude from the cut ends of the ducts, although commonly no macroscopic abnormality is seen. This absence of gross features makes selection of blocks challenging. Slicing and X-ray of each specimen slice is generally required to find and target the microcalcification with which the disease has presented.
Of particular relevance, therefore, is the finding that not all of the DCIS may be visible on the X-ray; 85% of the area may be seen mammographically for comedo/solid DCIS but only 50% of the area of micropapillary/cribriform disease may be evident.9 Mammography therefore tends to underestimate the size of DCIS, although this discrepancy is less than 20 mm in 80–85% of the cases if state-of-the-art mammography, including magnification views, is used.10 It is therefore important to sample widely, and especially to sample for microscopic examination those portions of the tissue immediately adjacent to the overt radiological microcalcifications, as non-calcifying DCIS may be present more peripherally. For the same reason, it is essential to sample the margins widely. Insufficient sampling, by merely targeting calcifications on specimen slice X-ray, will result in a tendency to underestimate the size of the DCIS and also the presence of disease close to, or at, the margins of excision.
It is certainly the case that the assessment of histological DCIS size may be challenging. Ductal carcinoma in situ is a unicentric proliferation, typically involving one duct system.10 Many pathologists seem to misinterpret areas of DCIS within a specimen as multiple foci of disease, seemingly without taking into account the three-dimensional pattern of the breast duct system. However, thorough examination with a stereoscopic technique of 60 mastectomy specimens showed a multifocal distribution (a gap of 4 cm or more between foci) in only one case.11 It is the case that 8% of DCIS had more than 10 mm ‘gaps’, most commonly seen in low grade disease, whereas poorly differentiated (high grade) DCIS tended to have a continuous growth pattern. Nevertheless, even if such gaps are present in an individual case, the size of DCIS reported should be the overall size of the disease process, rather than reporting individually the size of each focus.
Extremely helpful guidelines on specimen handling and reporting, including methods for the estimation of DCIS size have been outlined in the recent College of American Pathologists protocol document.12 This reminds the pathologist that if the DCIS extends to the margins of an orientated specimen (Figure 1), a minimum size can be given from the overall size of the tissue received. In other cases, large blocks can be invaluable particularly if taken along the maximum radiological extent of the calcification in the specimen X-ray (with the provisos noted above) and the maximum dimension of DCIS can then often be measured on a single large slide. Alternately, the size of DCIS can be assessed by the summation of the disease from continuous histological slides from an individual specimen slice, thus mapping the disease process. It can be helpful in such cases to either photograph or draw the specimen/specimen slices and annotate these with the site of each block, or indeed do the same on the specimen slice X-rays. If the maximum dimension of the DCIS lies across a number of slices of the specimen, this can be estimated by the assessment of the number of consecutive slices bearing the process,12 although this is inherently less accurate than direct measurement, due to estimation of the thickness of the specimen slices. Another alternative method has been described, which includes the calculation of the number of blocks bearing DCIS and multiplying this by either 0.3 or 0.4, the latter having been reported to give a more accurate estimate.13, 14
Pathological features
The microscopic heterogeneity of DCIS has led to the development of a number of systems for classification. Historically, DCIS has been classified on the basis of architectural pattern of the proliferation, including comedo, cribriform, micropapillary, solid or mixed subtypes. This system of categorization provides some information regarding likely extent of disease; for example, micropapillary DCIS is recognized to more often be multiquadrant (71%) than comedo-type disease (8%).15 Presentation may also be different, with comedo-type DCIS presenting most commonly mammographically compared with cribriform disease that is more commonly identified symptomatically, in some series.15 However, the reproducibility of this system of categorization based on growth pattern alone is problematic; lesions most frequently (62%) show a mixture of architectures, which is seen almost twice as often as the second most commonly seen pattern (solid, 31.9%).16 In addition, even an individual duct space may show an architectural pattern that is difficult to categorize. Typically, therefore, newer systems tend to be based on nuclear grade,12, 17 which is less commonly mixed (15.7%),16 some also incorporating the presence or absence of luminal necrosis.18
High-grade DCIS is formed from large, pleomorphic cells, often with multiple prominent nucleoli (Figure 1). Of particular assistance in classification is the assessment of the size of the nuclei compared with adjacent normal cells, typically either normal epithelial or red blood cells. The nuclei of high-grade DCIS are typically more than 2.5 red blood cells in diameter12 (Figure 2). Mitoses may be frequent. This grade of DCIS is often of solid architecture and tends not to show polarization of the cells and frequently bears central (comedo type) necrosis with or without associated microcalcification. Comedo necrosis may also, however, be seen in association with other architectural patterns (eg cribriform DCIS). The term ‘comedo DCIS’ is widely used in historical series but does not confer either a specific grade, or architecture, to the lesion and there is no consensus in the literature regarding the amount of central necrosis required, so reproducibility as a category of DCIS is questionable.
Conversely, low-grade DCIS is formed from evenly spaced small, regular cells with round monotonous nuclei. These are typically 1.5–2 red blood cells in diameter12 (Figure 3). The cells have well-defined cell boundaries and show polarization, for example, around micropapillae or cribriform structures. Mitoses are sparse and chromatin is usually finely dispersed. Nucleoli are typically not prominent.
Intermediate-grade DCIS is diagnosed when the lesion cannot be assigned to the high or low nuclear grade categories. The growth pattern is most often solid or cribriform and there is usually a degree of polarization. The nuclei show moderate pleomorphism, less than that seen in the high-grade cell disease but lack the monotony and regularity of size and spacing of the low-grade form. Perhaps not surprisingly, given the lack of precise features for categorization of DCIS as being of intermediate grade, this group has the poorest agreement in the UK National Health Service Breast Screening Programme (NHS BSP) External Quality Assurance (EQA) scheme with a κ value of 0.23, whereas the agreement for classification as high-grade disease is moderate (0.51).19
Despite the additional effort that is required to obtain better reproducibility of grading of DCIS, as noted above one advantage of classifying DCIS according to cytonuclear grade is that there is less commonly a variation within an individual lesion.16 Indeed, genetic studies suggest that low- and-high grade DCIS have different alterations, in keeping with the concept that this is a group of diseases.20 It should be noted, however, that an alternate finding is that a mixture of grades and biomarker expression may frequently be seen within an individual case of DCIS.21 How this can be reconciled with numerous data indicating that the cytonuclear grade of DCIS remains constant in progression from in situ to invasive, and indeed metastatic, disease,22 is difficult. Similarly, the genetic homology between DCIS and invasive carcinoma23, 24 that is reported is also contrary to the argument that an individual case frequently shows the heterogeneity that we recognize between lesions in different patients.
Grade of DCIS in surgical excisions relates to risk of local recurrence (see below) and to be relatively reproducible;19, 25 the histological grade of DCIS in core biopsy specimens is also clinically relevant with regard to the likelihood of there being radiologically unsuspected invasion.26 If, for example, a patient has more than 40 flecks of calcification on mammogram and core biopsy demonstrates high-grade DCIS, the risk of a radiologically occult invasive focus is 48%.26 Such patients may benefit from sentinel lymph nodes procedure at the time of primary breast surgery, rather than awaiting the discovery of invasive disease and requiring a second surgical procedure. Conversely, if axillary lymph nodes have been surgically removed and are found to bear metastatic tumor cells in a case in which only DCIS has been identified in the breast, clearly an invasive focus, although small, has been missed.
Prognosis
Grade of DCIS is related to the likelihood of progression to invasive carcinoma and the rapidity with which this is likely to occur.27 Although radiotherapy reduces the risk of local recurrence of disease by approximately half,28, 29 a number of prognostic markers of DCIS, in addition to cytonuclear grade, have been identified in randomized clinical trials and in other series.28, 30, 31, 32, 33, 34 The architectural growth pattern of the DCIS, the presence of necrosis, young age of patient and symptomatic detection have been reported to be poor prognostic factors for DCIS and to correlate with likelihood of local recurrence of disease, as has larger lesion size and in particular the presence of involved margins.28, 30, 31, 32, 33, 34
Thus, the presently recognized pathological prognostic factors for local recurrence of DCIS, nuclear grade, presence/absence of necrosis and architectural pattern(s), size/extent of DCIS and distance to excision margins/margin status should all be included in the pathology report. Use of a synoptic report is one technique that is widely used to ensure that all relevant features are included and allows for standardization of reporting of DCIS lesions. One such standardized proforma has been produced by the College of American Pathologists.12
Differential diagnosis
Reassuringly, despite the variability in microscopic appearance of DCIS, reproducibility of diagnosis is substantial, as shown in the UK NHS BSP EQA scheme (κ value=0.76).19 However, the differential diagnosis of DCIS includes other intraductal epithelial proliferations and lobular in situ neoplasia.
Atypical ductal hyperplasia (ADH) is a lesion showing some, but not all, of the features of DCIS.17, 35 Atypical ductal hyperplasia is diagnosed on the basis of architectural and cytological features but, in essence, if cellular changes of low-grade DCIS occupy two or more duct spaces, this should be reported as low-grade DCIS; if less extensive the process is classified as ADH. High-grade proliferations should be reported as high-grade DCIS, regardless of size or number of duct spaces involved. ADH does not have a high cytonuclear grade.
As typical of a clonal process, DCIS in general shows homogeneity of staining with a variety of markers; this can be valuable in diagnosis. Usual epithelial hyperplasia, conversely, commonly shows heterogeneity of expression, for example, of basal cytokeratins36 and generally also of estrogen receptor (ER).37 Low-grade DCIS is a luminal proliferation and shows uniform negativity for basal cytokeratins (CK5 and CK14) but high-grade DCIS, in particular, may rarely be of ‘basal’ phenotype.38, 39 Notably, however, the expression of basal cytokeratins in high-grade DCIS may be heterogeneous and focal (Figure 4) and should not be mistaken for the mosaic pattern typically seen in usual epithelial hyperplasia.
No markers are of assistance in distinguishing low-grade DCIS from ADH, which requires an assessment of the extent of the process and the cytological features; some authorities believe that these are in fact the same process, as shown by morphological, genetic and immunohistochemical similarities.
It can be difficult on rare occasions to distinguish low-grade solid DCIS from lobular carcinoma in situ (LCIS). Features in favor of DCIS are greater cellular cohesion with more evident cell borders and lack of intracytoplasmic lumina. E-cadherin immunohistochemistry can be helpful, but it should also be noted that combinations of both processes may exist and also that morphologically unequivocal LCIS may, rarely, express E-cadherin40 and ductal lesions may have reduced expression.
Microinvasive carcinoma is a lesion that has had varied definitions over time but which is now generally accepted as a lesion in which one or more separate foci of invasion are present, none of which measures more than 1 mm in diameter.12, 17 This rare lesion typically arises within high-grade DCIS and is very unusual in low-grade disease.41 However, high-grade DCIS may extend into the lobules as so-called ‘cancerization’ that may closely mimic microinvasive, or invasive (ie >1 mm), carcinoma and be misdiagnosed as such. Examination of the block at deeper levels may be helpful in recognizing the lobular architecture of cancerization of lobules or of sclerosing adenosis. Search for adenosis elsewhere in the specimen may also highlight the presence of this process in the specimen and avoid a misdiagnosis. Immunohistochemistry can assist in the identification of a myoepithelial layer around islands of such atypical epithelial cells; thus smooth muscle myosin, smooth muscle actin and p63, and even basement membrane markers, such as laminin and type IV collagen, may be valuable.
Conclusion
It is the responsibility of the pathologist not only to correctly diagnose DCIS, but also to provide important prognostic information, as for invasive carcinoma. Factors that should be included in a histological report of a case of DCIS include cytonuclear grade, presence or absence of necrosis, size of the lesion and distance to margins. As described, these typically require even more diligent and thorough macroscopic specimen handling than cases of invasive breast carcinoma, in additional to careful microscopic assessment. There is abundant research being undertaken on the profiles of precursor lesions of invasive breast cancer, but as yet no biomarkers have been convincingly shown, and validated, to predict the behavior of DCIS.42, 43 It remains the duty of the histopathologist to provide clinically important pathological information that guides patient treatment.
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Pinder, S. Ductal carcinoma in situ (DCIS): pathological features, differential diagnosis, prognostic factors and specimen evaluation. Mod Pathol 23 (Suppl 2), S8–S13 (2010). https://doi.org/10.1038/modpathol.2010.40
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DOI: https://doi.org/10.1038/modpathol.2010.40
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