Modern Pathology

FIGURE 1

FROM:

PLAG1 gene alterations in salivary gland pleomorphic adenoma and carcinoma ex-pleomorphic adenoma: a combined study using chromosome banding, in situ hybridization and immunocytochemistry

Carmo Martins, Isabel Fonseca, Lúcia Roque, Teresa Pereira, Catarina Ribeiro, Jörn Bullerdiek and Jorge Soares

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Figure 1.

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G-band and in situ hybridization analysis of a pleomorphic adenoma (case 3) with a t(5;8)(p13;q12–13). (a) Representative karyotype of the case; (b) FISH analysis with painting probes for chromosome 5 (green) and chromosome 8 (red), confirming the translocation; (c) FISH analysis with PLAG1 probe (red) with signal on normal chromosome 8 (arrow head) and with two small signals on the der(8) (arrow); (d) CISH analysis on a paraffin section, with PLAG1 probe; there are two adjacent brown spots, and a third spot confirming FISH observations.

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