INTRODUCTION

Gastric adenomas are relatively infrequent neoplasms which constitute at most approximately 10% of polyps arising in the stomach in Western populations (1). Despite this, adenomas hold importance because they can serve both as a direct precursor to gastric adenocarcinoma and as a marker for increased neoplastic risk elsewhere in the stomach (2, 3, 4, 5, 6, 7). Previous series have reported rates of carcinoma arising within adenomas that range from 2.5% to >50% (2, 3, 4, 5, 6, 7); in a 16-year retrospective review of gastric adenomas examined at The Johns Hopkins Hospital, we recently found that 15% of the polyps harbored carcinoma, and 10% of patients with adenomas had carcinomas elsewhere in the stomach (8).

Although all gastric adenomas are definitionally composed of polypoid, dysplastic epithelium, the morphologic features of adenomas vary. Most are formed from dysplastic intestinal-type epithelium that is comprised of a mixture of goblet cells with intestinal mucin and gastric (foveolar-type) cells with neutral mucin (Fig. 1; 2, 8, 9, 10). Much less commonly, adenomas are formed entirely or predominantly from dysplastic epithelial cells that resemble gastric (foveolar-type) cells both morphologically and by mucin immunohistochemistry (Fig. 2; 8, 9, 11). We found that intestinal-type gastric adenomas were more likely than those of foveolar-type to show high-grade dysplasia, to harbor intramucosal or invasive adenocarcinoma, and to arise in a background of extensive mucosal atrophy and intestinal metaplasia (8). A few other reports have also suggested that an intestinal versus foveolar phenotype of gastric adenomas correlates with greater malignant potential (2, 12).

FIGURE 1.

Histologic appearance of an intestinal-type gastric adenoma. Scattered goblet cells (arrowheads) and Paneth cells (arrows) are present. Epithelial dysplasia is classified as low grade in this adenoma (hematoxylin and eosin stain).

Full figure and legend (65K)

FIGURE 2.

Histologic appearance of foveolar-type gastric adenomas. A, foveolar-type adenoma with low-grade dysplasia in a patient with familial adenomatous polyposis (FAP; hematoxylin and eosin stain). B, periodic acid-Schiff/Alcian blue stain at pH 2.5 highlights the apical neutral mucin cap of the epithelial cells in this adenoma and its lack of goblet cells. C, the sporadic, non–FAP-associated foveolar-type adenomas analyzed in this study are histologically similar to FAP-associated adenomas (hematoxylin and eosin stain). D, higher power view demonstrating the presence of apical mucin caps in the lining epithelium (arrowheads). The nuclei are enlarged and crowded but show only mild pseudostratification, characteristic of low-grade dysplasia (hematoxylin and eosin stain).

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Genetic alterations contributing to the development and neoplastic progression of gastric adenomas have been studied with respect to the adenomatous polyposis coli (APC; 13, 14, 15), K-ras (6, 13, 15, 16, 17, 18), and p53 (6, 11, 13, 19, 20, 21, 22, 23, 24) genes and with respect to MSI (15, 18, 23, 24, 25, 26, 27, 28). However, only one study has separately evaluated intestinal-type and foveolar-type adenomas. In that series, Kushima et al. (11) found that p53 immunolabeling was significantly more common in intestinal-type than in gastric-type adenomas, suggesting that there might be genetic differences that underlie the phenotypic differences between these two types of lesions. In this study, we evaluate 18 gastric adenomas for differences in MSI and in APC, -catenin, and K-ras gene mutations between those of intestinal and foveolar phenotypes.

MATERIALS AND METHODS

Study Population

The study population consisted of 11 intestinal-type gastric adenomas and 7 foveolar-type adenomas removed at The Johns Hopkins Hospital between 1993 and 2001. All were endoscopically polypoid lesions projecting above the adjoining gastric mucosa (i.e., no "flat" or "depressed adenomas" were included). As previously described (8), we classified adenomas with the aid of periodic acid-Schiff/Alcian blue at pH 2.5 staining as intestinal type if there were Paneth cells or goblet cells (even focally) and as foveolar type if they were composed entirely of dysplastic foveolar cells with an apical cap of neutral mucin (Figs. 1, 2). The degree of epithelial dysplasia was categorized as either low grade or high grade according to previously published criteria (29, 30, 31). Adenoma size was obtained from either the endoscopic or gross pathology reports.

Although we have previously noted that most foveolar-type adenomas occur in patients with familial adenomatous polyposis (FAP; 8), the seven foveolar-type adenomas analyzed here were all sporadic adenomas removed from patients with no clinical indication of FAP.

DNA Extraction

Genomic DNA was obtained by microdissection of H&E-stained slides prepared from routinely processed, formalin-fixed, paraffin-embedded specimens. A 27.5-gauge needle tip was used for microdissection under a low-power, 4 objective. DNA was extracted according to protocol described elsewhere (32). Gastric adenomas were microdissected as well as normal control tissue that was obtained from nonpolypoid stomach mucosa (in 16 cases), duodenum (in 1 case), or esophagus (in 1 case).

Mutational Analysis of the APC, -Catenin, and K-ras Genes

Direct DNA sequencing was used to evaluate for mutations in APC, -catenin, and K-ras. For APC, genomic DNA was amplified by polymerase chain reaction (PCR) using four sets of overlapping oligonucleotide primers that span the mutation cluster region in exon 15 (A1: 5'-CAGACTTATTGTGTAGAAGA-3' and A2: 5'-CTCCTGAAGAAAATTCAACA-3' for codons 1260–1359; B1: 5'-AGGGTTCTAGTTTATCTTCA-3' and B2: 5'-TCTGCTTGGTGGCATGGTTT-3' for codons 1339–1436; C1: 5'-GGCATTATAAGCCCCAGTGA-3' and C2: 5'-AAATGGCTCATCGAGGCTCA-3' for codons 1417–1516; D1: 5'-ACTCCAGATGGATTTTCTTG-3' and D2: 5'-GGCTGGCTTTTTTGCTTTAC-3' for codons 1497–1596). For -catenin, PCR was performed using a primer pair (forward: 5'-ATGGAACCAGACAGAAAAGC-3' and reverse: 5'-GCTACTTGTTCTGAGTGAAG-3') that amplified a 200-bp fragment in exon 3 encompassing the region for glycogen synthase kinase-3 (GSK-3) phosphorylation and ubiquitin-mediated degradation of -catenin protein. For K-ras, PCR was performed with a primer pair (forward: 5'-GAGAATTCATGACTGAATATAAACTTGT-3' and reverse: 5'-TCGAATTCCTCTATTGTTGGATCATATTCG-3') that amplified a region in exon 1 spanning codons 12 and 13. All PCR reactions were performed on a GeneAmp PCR System 9700 (Applied Biosystems, Foster City, CA) in 50-L volumes that contained 2 L of genomic DNA, 10 mm dNTP mix, 2.25 U AmpliTaq Gold (Applied Biosystems), 0.125 U Pfu DNA Polymerase (Stratagene, La Jolla, CA), and 20 pmol of forward and reverse primers. PCR conditions for APC consisted of an initial denaturation step of 95° C for 10 minutes, 40 cycles (94° C for 1 minute, 55° C for 1 minute, and 68° C for 1.5 minutes for APC-B, -C, and -D primer pairs and 94° C for 1 minute, 52° C for 1 minute, and 68° C for 1.5 minutes for APC-A), followed by a final extension at 72° C for 10 minutes. For -catenin amplification, PCR conditions consisted of an initial denaturation at 95° C for 10 minutes, 40 cycles of 94° C for 1 minute, 58° C for 1 minute, and 72° C for 2 minutes, and a final extension at 72° C for 10 minutes. For K-ras, PCR reactions were performed with an initial denaturation at 94° C for 3 minutes, 40 cycles of 94° C for 1 minute, 50° C for 1 minute, and 72° C for 1 minute, and a final extension at 72° C for 7 minutes.

PCR products were cleaned using shrimp alkaline phosphatase and exonuclease I (Amersham, Buckinghamshire, United Kingdom). The products were then sequenced on an ABI Prism 3700 DNA Analyzer (Applied Biosystems) using the ABI Prism Big Dye Terminator Cycle Sequencing Kit (Applied Biosystems). For all three genes, the same primers were used for both amplification and sequencing. Sequencing data were analyzed using GeneScan Analysis software (Applied Biosystems) as per the manufacturer's protocol. Mutations were verified in both forward and reverse directions.

Chromosome 5q Allelic Loss

Allelic loss on chromosome 5q was evaluated by microsatellite assays using three microsatellite markers: D5S82, D5S299, and D5S346. Assays were performed by fluorescent-labeled PCR amplification using forward primers labeled with 6-FAM (Applied Biosystems) and unlabeled reverse primers. PCR reactions were performed in 15-L volumes that contained 40 ng of genomic DNA, 9 L of ABI Prism True Allele PCR Premix (Applied Biosystems), 5 pmol of 6-FAM-labeled forward primer, and 10 pmol of unlabeled reverse primer. Cycling conditions consisted of denaturation at 95° C for 6 minutes; 45 cycles of 94° C for 45 seconds, 55° C for 45 seconds, and 72° C for 1 minute; and a final extension at 72° C for 30 minutes. The PCR products were subsequently diluted with 30 L of H₂O, and a 1.0-L aliquot of each diluted fluorescent-labeled PCR product was combined with 12 L of formamide and 0.5 L of GeneScan 400HD (ROX) size standard (Applied Biosystems). The samples were then capillary electrophoresed on an ABI 3700 DNA Analyzer and analyzed using GeneScan Analysis software. Allelic loss was defined as a 50% reduction in the height of a heterozygous peak as compared with normal control DNA in one or more informative microsatellite markers.

MSI Analysis

Evaluation for MSI was performed using the five microsatellite loci (D2S123, D5S346, D17S250, Bat-25, and Bat-26) recommended by the 1997 National Cancer Institute (NCI)-sponsored consensus conference (33). PCR reaction mixtures and cycling conditions for D2S123, D17S250, Bat-25, and Bat-26 were identical to those described above for 5q allelic loss (already including D5S346). Primer sequences and interpretation of the chromatograms were in accordance with those previously described in detail by Berg et al. (34). High-level MSI was defined as shifts in at least two of five microsatellite loci (40%); low-level MSI as a shift in only one locus (20%); and microsatellite stable, as when none of the loci were shifted, as per the NCI criteria (33).

Statistical Analysis

Fisher's exact test was used to evaluate differences in the frequencies of genetic alterations among intestinal-type and gastric-type adenomas. Two-tailed P values of <.05 were considered statistically significant.

RESULTS

The histopathologic and molecular findings in the 18 gastric adenomas are summarized in Table 1.

TABLE 1 - Genetic Alterations in Intestinal-Type and Foveolar-Type Gastric Adenomas.

Full table

Intestinal-Type Adenomas

The 11 intestinal-type adenomas occurred in 6 females and 5 males with a mean age of 75.5 years (range, 62–93 years). As previously described (8), histologic evaluation of the surrounding gastric mucosa revealed that these adenomas arose in the setting of intestinal metaplasia, including extensive antral-predominant intestinal metaplasia in eight patients, extensive corpus-predominant intestinal metaplasia (i.e., autoimmune gastritis) in two patients, and focal intestinal metaplasia only in one patient. In 10 patients, the polyps were endoscopically or surgically resected and examined in their entirety, whereas in the remaining patient the lesion was biopsied only. Polyp size in these 10 recorded cases ranged from 0.3–3 cm, with a mean of 1.35 cm. The degree of epithelial dysplasia was classified as low grade in 6 adenomas and as high grade in 5 adenomas. In addition, 2 of the adenomas with high-grade dysplasia contained foci of intramucosal carcinoma, but for the purposes of this study only the adenoma precursors were microdissected and analyzed.

Alterations in the APC tumor-suppressor gene were the most common genetic alteration identified in intestinal-type adenomas. APC mutations were present in 5 of 11 (45.5%) cases and 5q allelic loss in 3 of 9 (33.3%) informative cases. The APC mutations included two stop-codon mutations and three frameshift (insertion or deletion) mutations, and therefore all would be expected to result in APC protein truncation. In one of the adenomas, biallelic inactivation of APC (truncating mutation plus loss of the normal allele) could be demonstrated.

MSI was present in 3 of 11 (27.3%) intestinal-type adenomas and constituted high-level MSI in all 3 cases (Fig. 3). Among the adenomas studied here, the presence of MSI and APC alterations (mutation and/or allelic loss) appeared to be mutually exclusive. Overall, therefore, 10 of 11 (90.9%) intestinal-type adenomas demonstrated either APC alterations or MSI, but not both. Notably, neither MSI nor APC alterations correlated with adenoma size or degree of dysplasia (Table 1); the only adenoma in this group that did not demonstrate either MSI or APC alteration was a 1.4-cm polyp (thus approximating the mean size among the group of intestinal-type adenomas) with high-grade dysplasia and intramucosal carcinoma.

FIGURE 3.

High-level microsatellite instability in an intestinal-type adenoma. Representative allelic shifts in D2S123, D17S250, and Bat-25 are illustrated (Case IA-9).

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In contrast to the relative frequency of APC alterations in intestinal-type adenomas, K-ras oncogene mutations were present in only two (18.2%) cases, representing activating codon 12 and codon 13 missense mutations, respectively (Fig. 4). Both of these K-ras mutations occurred in adenomas that also contained APC alterations. No -catenin mutations were identified, either among adenomas with or without APC mutations.

FIGURE 4.

K-ras oncogene mutation in an intestinal-type adenoma. A codon 12 GGT(glycine) AGT(serine) mutation is present (Case IA-6). The wild-type (normal) mucosa at top shows only the normal GGT DNA sequence at codon 12 of K-ras, whereas the intestinal-type adenoma at bottom contains a G (black peak) to A (green peak) point mutation that will result in the substitution of serine for glycine in the K-ras protein. The sequencing chromatogram for IA-6 shows overlap of the green and black peaks, reflecting the dominant positive nature of the mutation and its presence in only one of the K-ras alleles.

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Foveolar-Type Adenomas

The seven foveolar-type adenomas occurred in four females and three males who had a mean age of 65.9 years (range, 54–80 y). Foveolar-type adenomas were in general smaller lesions than intestinal-type adenomas, with a mean size of 0.5 cm (range, 0.3–1 cm). As was described elsewhere (8), in contrast to intestinal-type adenomas, these foveolar-type adenomas were most frequently associated with a nonatrophic and nonintestinalized mucosal background. In only one case (FA-1) was there any metaplasia or atrophy of the surrounding mucosa, a patient with autoimmune gastritis, extensive associated pseudopyloric metaplasia of the gastric body/fundus, and a 1-cm foveolar-type adenoma in the fundus. Epithelial dysplasia was low grade only in all of the foveolar-type adenomas, and none harbored intramucosal or invasive carcinoma.

Alterations in APC were present in three of seven (42.9%) foveolar-type adenomas, including two adenomas with 5q allelic loss (Fig. 5) and one adenoma with an intragenic APC mutation (Fig. 6). This latter adenoma, FA-1, was the 1-cm fundus polyp in the patient with autoimmune gastritis, representing the only foveolar-type adenoma to arise in association with background mucosal atrophy; it harbored a 2-bp deletion frameshift mutation that would be expected to result in protein truncation. This adenoma also harbored an activating codon 12 K-ras mutation; the other five foveolar-type adenomas in which K-ras sequencing was completed contained only wild-type sequences. All seven foveolar-type adenomas were microsatellite stable. Only wild-type -catenin sequences were present in six amplifiable cases.

FIGURE 5.

Chromosome 5q allelic loss in a foveolar-type adenoma. Loss of the shorter alleles (arrows) is seen for both microsatellite markers D5S299 and D5S346 (Case FA-3).

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FIGURE 6.

Frameshift APC gene mutation in a foveolar-type adenoma. Sequencing chromatogram shows a 2-bp (AG) deletion in a tract spanning codons 1463–1465 (Case FA-1). The wild-type (normal) mucosa at top shows the expected DNA sequence for this portion of exon 15 of the APC gene. In the FA-1 polyp at bottom, the DNA sequencing chromatogram shows overlapping bases after the AG deletion because the wild-type and mutated alleles are of different lengths.

Full figure and legend (72K)

Foveolar-type adenomas demonstrated a lower overall frequency of detectable clonal genetic alterations than did intestinal-type adenomas (i.e., 10 of 11 intestinal-type adenomas contained alterations in APC, K-ras, or MSI versus only 3 of 7 foveolar-type adenomas; P = .047). However, there were no particular statistically significant differences between gastric-type and intestinal-type adenomas with respect to alterations in APC, K-ras, or -catenin, or with respect to the presence of MSI.

DISCUSSION

Unlike colorectal carcinogenesis, the adenoma-carcinoma sequence is an uncommon pathway for gastric adenocarcinoma development, and most intestinal-type gastric carcinomas do not appear to arise from adenoma precursors (13, 15, 35, 36). Not only do most intestinal-type gastric carcinomas lack an identifiable adenoma precursor histologically, but divergent patterns of allelic loss (35, 36) and molecular alterations in genes including APC (13, 15) exist between most adenomas and most carcinomas. Nevertheless, that gastric adenomas can serve as the direct precursors to adenocarcinoma is well documented. Estimations of the frequency of such neoplastic progression have ranged widely in the literature, from 2.5% to >50% (2, 3, 4, 5, 6, 7).

We recently reported that 15% of gastric adenomas examined at The Johns Hopkins Hospital over a 16-year period contained either intramucosal (7%) or infiltrating (8%) carcinoma (8). However, there was a striking difference between adenomas with an intestinal-type phenotype and those with a foveolar phenotype. Whereas 23% of intestinal-type adenomas harbored carcinoma, none of the foveolar-type adenomas did (8). The foveolar-type adenomas in that study occurred in two distinct scenarios: FAP associated (in the majority of cases) and sporadic. The fact that foveolar-type adenomas rarely undergo neoplastic progression is further reinforced by epidemiologic evidence, which has failed to show a statistically significantly increased risk of gastric carcinoma among Western patients with FAP, despite the increased incidence of gastric adenomas in these patients (37). We therefore hypothesized that there might be different genetic alterations in foveolar-type and intestinal-type adenomas to account for this divergent biologic behavior.

We found that intestinal-type adenomas were more likely than foveolar-type adenomas to harbor a detectable clonal genetic alteration in APC, MSI, and/or K-ras (91% versus 43%, respectively). In particular, 5 of 11 intestinal-type adenomas had truncating APC mutations, but only 1 of 5 amplifiable foveolar-type adenomas did. In addition, 3 of 11 intestinal-type adenomas showed high-level MSI, but all 7 foveolar-type adenomas were microsatellite stable. Furthermore, differences in adenoma size did not account for the presence or absence of APC mutations or MSI. However, despite the higher frequency of detectable genetic alterations in intestinal-type versus foveolar-type adenomas, no particular alteration (i.e., truncating APC mutation, 5q allelic loss, MSI, or K-ras mutation) reached statistical significance. This may be due to the relatively low number of foveolar-type adenomas available for study, because sporadic foveolar-type adenomas are very uncommon lesions.

Previous studies of genetic alterations in APC, K-ras, and MSI in gastric adenomas have not generally specified the epithelial phenotype of the adenomas; however, because the vast majority of non–FAP-associated gastric adenomas arise in the setting of background intestinal metaplasia and show at least focal intestinal-type differentiation, it is likely that the genetic changes reported in these previous studies reflect mainly those seen in intestinal-type adenomas. Only two prior investigations of APC in polypoid gastric adenomas have been reported and have shown mutation frequencies of 20% (6 of 30 adenomas from Japanese patients; 14) and 78% (25 of 32 adenomas from Korean patients; 15), respectively. In neither study did degree of dysplasia or adenoma size correlate with the presence of APC mutations, suggesting that, as in the colorectum, APC alterations are early neoplastic events. The lack of correlation that we also observed between APC alterations and adenoma size or dysplasia grade in this study also supports this. Interestingly, unlike colorectal adenocarcinomas, which arise predominantly from adenoma precursors and typically harbor truncating APC mutations, only a minority of differentiated gastric adenocarcinomas contain APC mutations (13, 15, 38), an observation suggesting that most gastric adenocarcinomas do not arise from adenoma precursors. Lee et al. (15), for example, found somatic APC mutations in only 6% of gastric adenocarcinomas and hypothesized that dysplastic or adenomatous lesions with APC mutations were actually less likely to progress to carcinoma. In the current study, neither of the two gastric adenomas that harbored adenocarcinoma (intramucosal carcinoma in both) contained detectable APC mutations or 5q allelic loss.

Among colorectal neoplasms that lack detectable APC mutations, two alternative genetic alterations may be found—-catenin oncogene mutations and MSI. Mutations that disrupt phosphorylation sites involved in regulating normal -catenin protein degradation can substitute for APC mutations and theoretically should stimulate similar downstream targets of the APC/-catenin pathway, including transcriptional activation of cyclin D1 and c-myc (39, 40, 41). Approximately half of colonic adenomas lacking APC alterations instead harbor stabilizing -catenin mutations (42). Notably, -catenin mutations are more prevalent among small adenomas than larger adenomas or colonic adenocarcinomas (43, 44). This latter observation led us to hypothesize that -catenin mutations might be present in gastric adenomas without APC alterations, particularly the small, foveolar-type adenomas. However, none of the 17 gastric adenomas sequenced here for -catenin contained mutations. Only one previous study has evaluated for -catenin mutations in gastric adenomas, and similarly reported no -catenin mutations among 12 (intestinal-type) adenomas from Korean patients (15). -catenin also does not appear to be a frequent mutational target in gastric carcinomas, having been reported in only 0–7.5% of cases studied (45, 46, 47, 48, 49, 50).

A second pathway, MSI, was present in 3 of 11 (27.2%) intestinal-type adenomas, all of which were MSI high. Several previous studies have reported prevalences of MSI in gastric adenomas ranging from 8% to 28.6% (15, 23, 24, 26, 27), with the majority of MSI-high cases associated with decreased hMLH1 expression due to hypermethylation of the hMLH1 gene promoter, as occurs in sporadic microsatellite-unstable colorectal neoplasms (26). One recent study of gastric carcinomas identified MSI by Bat-25/Bat-26 analysis in only 8% of intestinal-type carcinomas but in 57% of carcinomas with a foveolar phenotype (foveolar-type carcinomas expressing gastric mucins; 51). We therefore hypothesized that foveolar-type adenomas might also have a high frequency of MSI. However, all seven foveolar-type adenomas evaluated here were microsatellite stable, further suggesting a different molecular pathogenesis between foveolar-type adenomas and foveolar-type adenocarcinomas.

Among colorectal neoplasms, both APC/-catenin pathway alterations and MSI represent early neoplastic events, whereas K-ras oncogene mutations and p53 tumor suppressor gene mutations are secondary, subsequent alterations in the neoplastic pathway. We found codon 12 and 13 K-ras mutations in only 3 of 18 gastric adenomas (16.7%), a prevalence not significantly different from the range (0–16%) in previously studied gastric adenomas (6, 13, 15, 16, 17, 18). Isogaki et al. (18) found that the K-ras mutational frequency increased with higher degrees of dysplasia in their set of 50 adenomas. Similarly, Tsuchiya et al. 16) found that the K-ras mutations present in 10.5% of their set of 38 adenomas were not homogeneously distributed (i.e., could be present only focally within different sites from the same adenoma). The three K-ras mutations detected in the current study were present only in adenomas of 1 cm (1 cm, 1.5 cm, and 2 cm), supporting that these are secondary genetic events in neoplastic progression.

In summary, the findings in this study suggest that gastric adenomas (particularly those of intestinal phenotype), like colorectal adenomas, arise predominantly through mutational inactivation of the APC tumor suppressor gene, with MSI representing a less frequent pathway. Adenomas of pure foveolar phenotype are encountered only rarely. They were less likely to harbor detectable clonal genetic alterations than those of intestinal type, although no particular genetic alteration reached statistical significance.

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