FIGURES AND TABLES

FIGURE 1.

A–H, morphological and immunohistochemical features of a spindle cell (A, C, E, G) and an epithelioid (B, D, F, H) gastrointestinal stromal tumor. A, the spindle cells are arranged in storiform and herringbone patterns and have blunt-ended nuclei. B, the epithelioid cells are arranged in organoid clusters and have round nuclei and abundant cytoplasm. Both subtypes express CD34 (C and D), bcl-2 (E and F), and the Kit receptor (G and H). Magnifications, 240.

FIGURE 2.

Predicted amino acid sequences in Exons 9, 11, and 13. Numbers shown above the wild-type amino acid sequence indicate codons. Point mutations and changed amino acids are indicated in an empty square, insertions in a black square. Deleted amino acids are shown by dashes (—).

FIGURE 3.

Single-strand conformational polymorphism analysis and sequence of Exon 11 in GISTs. Case 1 carries a 3-bp deletion corresponding to the deletion of Asp-579. The point mutation in Case 6 leads to the replacement of Val-559 by Asp. In Case 7, Trp-557 and Lys-558 are deleted; in Case 11, a deletion of 9 bp and concurrent insertion of 3 bp leads to the replacement of Gln-556, Trp-557, Lys-558, and Val-559 by His and Thr. In Case 10, two amino acids (Gln and Leu) are inserted at Codon 577.

FIGURE 4.

Single-strand conformational polymorphism analysis and sequence analysis of Exons 9 and 13 in gastrointestinal stromal tumors. Case 41: Exon 9 shows a duplication of six nucleotides encoding for Ala-502 and Tyr-503. Case 47: In Exon 13, the mutation of A to G leads to the substitution of Lys-642 by Glu.

FIGURE 5.

A review of mutations in Exon 11 in 150 cases published until now. On the right side, number of cases per mutation is indicated; under ref., authors are symbolized as follows: *Ernst et al. (4), +Hirota et al. (9), <Lasota et al. (14), =Miettinen et al. (24), ⁁Moskaluk et al. (28), Ñakahara et al. 1998 (29), $Sakurai et al. 1999 (31), §Taniguchi et al. 1999 (28), and >Wardelmann et al. 2002. The predominant type of mutation is a simple deletion of 1 to 15 amino acids.

FIGURE 6.

Frequency of mutations per codon published until now (including our own cases). Each square symbolizes one case with an amino acid change or loss in this codon. A clustering of mutations is observed in the region between Codons 553 and 561 (128/150 cases, i.e., 85.3%).

TABLE 1 - Immunohistochemistry: Antigens, Antibodies, Pretreatment, Dilutions, and Sources.

TABLE 2 - Primers and Product Sizes of the Polymerase Chain Reaction Amplicons for Single-Strand Conformational Polymorphism Analysis.

TABLE 3 - PCR and Gel Conditions for SSCP Analysis of the Different Amplicons.

TABLE 4 - Clinical Data (36 GastrointestinalStromal Tumors).

TABLE 5 - Immunohistochemical Results (36 Gastrointestinal Stromal Tumors).