INTRODUCTION

Low-grade B-cell lymphomas of mucosa-associated lymphoid tissue (MALT) typically arise in extra-nodal organs that physiologically do not contain organized lymphoid tissues such as the gastrointestinal (G-I) tract, thyroid gland, orbits, salivary glands, respiratory tract, and genitourinary tract (1). MALT lymphomas tend to remain localized to the site of origin for long periods (2, 3). Lymph nodal involvement is rarely observed and only in cases of gastric low-grade MALT lymphoma invading the proper muscle layer of the stomach or deeper (4). However, MALT lymphomas sometimes disseminate to the MALT without lymph node involvement (5, 6, 7), as cutaneous lymphomas tend to spread to the skin without lymph nodal lesions. These observations imply that mechanisms controlling normal mucosal lymphocyte traffic may also be operational in MALT lymphomas.

Lymphocyte homing into the selective lymphoid tissues is regulated by adhesion molecules expressed by the lymphocytes (homing receptors) and their endothelial ligands, vascular addressins (8, 9). It has been shown in mice that 47 integrin, which is expressed on mucosal lymphocytes, primarily mediates lymphocyte traffic to the intestinal mucosa through interactions with mucosal addressin cell adhesion molecule-1 (MAdCAM-1), which is expressed on high endothelial venules (HEVs) in the gut-associated lymphoid tissues (GALT; 10, 11). This has also been found to be the case in humans (12). The human 47 integrin is widely expressed by leukocyte subsets in the peripheral blood, organized lymphoid tissue, intestinal lamina propria, and lung parenchyma (12, 13, 14, 15, 16, 17). Its corresponding ligand, human MAdCAM-1, has recently been cloned and found to be expressed primarily in the small intestine and, to a lesser extent, in the colon and spleen (18, 19). Malignant lymphomas are expected to show the same expression pattern as that observed in corresponding counterparts of the nonneoplastic lymphoid tissues. In fact, 47 integrin is preferentially expressed in gastrointestinal low-grade MALT lymphomas (28). 4 integrin is also expressed on leukocytes as a heterodimeric integrin with the 1 integrin chain. 41 (VLA-4) is a ligand for VCAM-1 (20) and is involved in B-lymphocyte adhesion to germinal centers (21) but is not involved in lymphocyte homing to mucosal sites.

L-selectin regulates lymphocyte homing to the peripheral lymph node through interactions with the peripheral lymph node vascular addressin (PNAd) expressed on HEVs (8, 22). PNAds include GlyCAM-1 (23) and CD34 (24). Furthermore, L-selectin has been shown to bind to sialyl-Lewis^x (25), which is also expressed on human HEV (26), and to a mucinlike domain of MAdCAM-1 (27). Therefore, it is possible that L-selectin is also involved in mucosal lymphocyte trafficking. L-selectin is expressed on germinal-center B cells and is consistently expressed on mantle zone B-cells (29). Although extrafollicular B cells and plasma cells in tonsils and lymph nodes are L-selectin positive, those within the lamina propria of gastrointestinal tract show a variable expression of L-selectin (29). As for malignant lymphomas, it has been reported that 50 to 80% of low-grade gastric lymphomas express L-selectin (29, 30).

Recent studies have revealed expression of 47 integrin and L-selectin in G-I MALT lymphomas (28, 29, 30), but there are few data about the homing receptors in MALT lymphomas of other organs. In the present study, we examined the expression patterns of 47 integrin, L-selectin, and VLA-4 in MALT lymphomas of the G-I tract, thyroid, and ocular adnexa. We also identified changes in their expression pattern associated with the high-grade transformation of low-grade MALT lymphoma.

MATERIALS AND METHODS

Tissue Samples

We examined 41 cases of low-grade MALT lymphoma (19 gastric, 3 intestinal, 10 ocular adnexal, and 9 thyroid), 9 cases of high-grade MALT lymphoma (5 gastric and 4 thyroid), and 19 cases of diffuse large B-cell lymphoma (DLBL; 8 gastric, 6 intestinal, 4 ocular adnexal, and 1 thyroid) selected from the files of the Second Department of Pathology, Okayama University Medical School. Low-grade MALT lymphomas were diagnosed according to the criteria described by Isaacson (1; Fig. 1A–B). We defined high-grade MALT lymphomas as combined lymphomatous foci of high-grade as well as low-grade components (Fig. 2A and D). Fresh tissue samples were obtained from the surgically resected materials, snap-frozen in liquid nitrogen, and stored at -80° C until used. For histopathological diagnosis, the tissue specimens were fixed in buffered formalin and paraffin embedded.

FIGURE 1.

Immunohistochemical staining of 47 integrin and L-selectin in the low-grade gastric MALT lymphoma. A and B, histological features, 35 , 370 . C, 47 integrin, 160 ; D, L-selectin, 160 .

Full figure and legend (36K)

FIGURE 2.

Immunohistochemical staining of 47 integrin and L-selectin in the high-grade gastric MALT lymphoma. A–C, low-grade components; D–F, high-grade components. A and D, histological pictures, 270 ; B and E, 47 integrin, 160 ; C and F, L-selectin, 160 . The high-grade components of high-grade MALT lymphoma are negative for either 47 integrin or L-selectin (E and F), whereas intermingled low-grade components and some intermingled nonneoplastic lymphocytes (upper right field) show positive immunoreactivity.

Full figure and legend (46K)

Immunohistochemistry

Immunohistochemical staining was performed on acetone-fixed cryostat sections for 47 integrin, L-selectin, and VLA-4 or on formalin-fixed, paraffin-embedded sections for MAdCAM-1 by the indirect immunoperoxidase method using dextran polymer–conjugated secondary antibody labeled with peroxidase (EnVision⁺, DAKO Japan, Kyoto, Japan). In brief, 4–6-m-thick sections were blocked with 10% goat serum or 10% skim milk and then incubated with the primary antibodies listed in Table 1 for 90 minutes at room temperature. The specimens were then treated with the EnVision⁺ immunostaining system according to the manufacturer's instructions. Diaminobenzidine was used for color development, and hematoxylin, for counterstaining.

TABLE 1 - The Monoclonal Antibodies Used.

Full table

For double immunofluorescence, acetone-fixed frozen sections were blocked with 10% skim milk for 15 minutes and then treated with anti-47 integrin monoclonal antibody (mAb) for 90 minutes at room temperature. After washing with 0.05% Tween 20-containing phosphate buffered saline (Tween-PBS), the specimens were treated with 1:100-diluted rhodamine–labeled goat (Fab') 2 anti-mouse IgG (Leinco Technologies Inc., Ballwin, MO) for 30 minutes at room temperature. After washing with Tween-PBS and blocking with 10% normal mouse serum, they were treated with fluorescence isothiocyanate–labeled anti-CD 20 or anti-CD3 mAbs (DAKO) for 30 minutes at room temperature. Haexist 1:500 was used for nuclear staining. Simultaneous detection of both 47 integrin and L-selectin was performed by the double immunofluorescence method, as described elsewhere (31).

Statistical Analysis

A statistical comparison of 47 integrin or L-selectin immunoreactivity was performed by the ² test using a statistical software package (Fisher's exact test).

RESULTS

Expression of 47 Integrin in MALT Lymphoma

The immunoreactivity of MALT lymphomas and DLBL for 47 integrin, L-selectin, and VLA-4 is summarized in Table 2. Most of the low-grade gastric MALT lymphoma and a low-grade component of high-grade gastric MALT lymphoma were positive for 47 integrin (Fig. 1C and 2B). In contrast, a high-grade component of high-grade MALT lymphoma and DLBL rarely expressed 47 integrin (Fig. 2E), and this difference was statistically significant. 47 integrin was also found to be expressed in all cases of intestinal low-grade MALT lymphoma and in a small proportion of ocular adnexal and thyroid low-grade lymphoma. Nonneoplastic plasma cells in the lamina propria were positive for 47 integrin but negative for L-selectin. Some T cells were also positive for 47 integrin. MALT lymphoma cells frequently show plasmacytic differentiation and are sometimes intermingled with many reactive T cells; it is therefore critically important to determine whether 47 integrin-positive cells are centrocyte-like cells of MALT lymphoma, nonneoplastic plasma cells, or intermingled T cells. For this purpose, gastric low-grade MALT lymphoma tissues were immunostained by the double immunofluorescence method using fluorescence isothiocyanate–labeled anti-CD3 or CD20 mAb and anti-47 integrin mAb combined with rhodamine-labeled anti-mouse IgG antibody. Figure 3A–B clearly shows that most 47 integrin–positive cells were concomitantly positive for CD20 and negative for CD3; they were thus easily discriminated from intermingled small-sized T cells.

FIGURE 3.

Double immunofluorescence for 47 integrin and CD20, CD3, or L-selectin in low-grade gastric MALT lymphoma. (a), 47 (rhodamine), and CD20 (fluorescence isothiocyanate), 400 . (b), 47 (rhodamine) and CD3 (fluorescence isothiocyanate), 400 . (c), 47 (fluorescence isothiocyanate) and L-selectin (rhodamine), 600 . Double-positive cells are stained orange.

Full figure and legend (76K)

TABLE 2 - Expression of 47 Integrin, l-Selectin, and VLA-4 in Gastrointestinal, Occular Adnexal, and Thyroid Lymphoma.

Full table

Expression of L-Selectin in MALT Lymphomas

We used two kinds of mAbs to L-selectin, DREG-56 and FMC46; FMC46 showed better reactivity, and the stainability of the specimens was evaluated from the results of staining with FMC46 (Table 2). L-selectin was frequently expressed in low-grade MALT lymphoma of the G-I tract and ocular adnexa and in a low-grade component of high-grade gastric MALT lymphoma (Figs. 1D and 2C), in contrast, a high-grade component of gastric high-grade MALT lymphoma tended to lose L-selectin expression (Fig. 2F). Low-grade MALT lymphomas of the thyroid infrequently expressed L-selectin, with L-selectin being detected in half of DLBL cases of the stomach and ocular adnexa but in none of DLBL cases of the gut and thyroid.

Expression of VLA-4 in MALT Lymphomas

VLA4 was detected in only a small proportion of the cases examined, but especially in cases of low-grade MALT lymphoma (Table 2). In gastric low-grade MALT lymphomas, three of four VLA-4⁺ cases were also positive for both 47 integrin and L-selectin.

Coexpression of 47 Integrin and L-Selectin

In the low-grade MALT lymphomas of the G-I tract, all 22 cases examined expressed 47 integrin, and 18 cases also expressed L-selectin. In the ocular adnexa and thyroid, approximately half of the cases of low-grade MALT lymphoma expressed either 47 integrin or L-selectin. In the cases expressing both 47 integrin and L-selectin, both adhesion molecules were primarily coexpressed at individual cellular levels, as evidenced by the double immunostaining (Fig. 3C), and only a few 47 integrin -positive cells were negative for L-selectin 2.

Expression of MAdCAM-1

MAdCAM-1 was consistently expressed on HEVs in the gastric mucosa of chronic gastritis and also in the lymphomatous lesions of gastric MALT lymphoma but not on HEVs in gastric DLBL (Fig. 4). HEVs in low-grade MALT lymphoma tissues of the ocular adnexa were negative for MAdCAM-1. In the thyroid, most lesions of chronic thyroiditis and about a half of low-grade MALT lymphoma lesions expressed MAdCAM-1 on the HEVs (data not shown).

FIGURE 4.

Immunohistochemical staining of MAdCAM-1 in the gastric mucosa of chronic gastritis (A) and in the lymphomatous lesions of low-grade MALT lymphoma (B), high-grade MALT lymphoma (C), and DLBL (D) of the stomach, 220 .

Full figure and legend (43K)

DISCUSSION

In this study, we observed preferential expression of 47 integrin and L-selectin on lymphoma cells of low-grade MALT lymphoma and low-grade components of high-grade MALT lymphoma of the G-I tract. Plasma cells in the lamina propria of the human stomach and small intestine strongly expressed 47 integrin but were negative for L-selectin, as described elsewhere (30). Dogan et al. (30) have reported that the majority of marginal-zone B cells of the human GALT express low levels of 47 integrin and high levels of L-selectin. MALT lymphoma cells are derived from marginal-zone B cells of the memory phenotype. Therefore, it is reasonable that low-grade MALT lymphoma cells of the G-I tracts consistently expressed variable levels of 47 integrin and L-selectin. HEVs in the nonlymphomatous G-I mucosa and gastric MALT lymphoma tissues were positive for MAdCAM-1 as reported (30; Fig. 4). The interaction between the 47 integrin expressed by lymphocytes and MAdCAM-1 expressed by HEVs mediates lymphocyte homing to GALTs. This physiological homing mechanism may also function in MALT lymphomas and may explain the biological characteristic of MALT lymphomas in which they tend to remain localized to the site of origin for long periods.

Our results showing that low-grade MALT lymphoma cells of the G-I tract consistently express 47 integrin are comparable with those reported previously by Drillenburg et al. (28). High-grade components of gastric MALT lymphoma and DLBL cells were found to be consistently negative for 47 integrin. Thus, G-I MALT lymphoma cells may lose their expression of 47 integrin after progression to a high-grade malignancy. L-selectin was also found to be less frequently expressed in high-grade than in low-grade MALT lymphoma cells.

In contrast to the present data, Doganet al.,30) have reported that low-grade MALT lymphoma cells in the stomach are 47 integrin^-, L-selectin⁺, whereas the intestinal secondary lymphoma cells are 47 integrin⁺, L-selectin^-. They claimed that 47 integrin positivity in gastric low-grade MALT lymphoma may be overestimated by the presence of 47 integrin⁺ plasma cells in the lamina propria and that 47 integrin⁺ reactive T cells intermingling with lymphoma cells. In fact, some cases of gastric low-grade MALT lymphoma have shown substantial numbers of reactive T cells. In our series, however, lymphomatous lesions with only a few intermingled T cells also showed 47 integrin positivity. Even in the T cell–rich area, double immunofluorescent staining of CD3/47 integrin and CD20/47 integrin showed that CD20⁺ B cells were positive for 47 integrin.

In the ocular adnexa and thyroid, 47 integrin was less frequently expressed, even in low-grade MALT lymphomas. This evidence suggests that another homing system may operate in these organs, although MAdCAM-1 is frequently expressed in HEVs of the thyroid. VLA-4 was expressed only in a small proportion of low-grade MALT lymphomas and in none of the DLBL. Therefore, VLA-4 may not play a major role in the growth of lymphoma in the mucosal site.

In conclusion, the present study shows that 47 integrin and L-selectin may be involved in the mucosal homing of low-grade MALT lymphoma cells in the G-I tract and that the expression of 47 integrin is lost in the course of high-grade progression.

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Acknowledgements

This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan (Grant-in-Aid for Exploratory Research 09877037).

We thank Dr. M.J. Briskin of LeukoSite Inc. for the generous gift of monoclonal antibody MAdCAM-1. We are also thankful to the Okayama University Medical School Hospital and the Aichi Cancer Center for providing the cases materials. We also thank Ms. Motochika for her help with the photography and Ms. Okabe for her help with the immunohistochemistry.