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Leptin and Leptin Receptor mRNA are Widely Expressed in Tumors of Adipocytic Differentiation

Andre M Oliveira, Antonio G Nascimento and Ricardo V Lloyd

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FIGURE 1 - Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, please contact help@nature.com or the author

FIGURE 1.

Cytoplasmic immunoreactivity for leptin in lipoma (A), hibernoma (B), and well-differentiated liposarcoma (C and D). Nonspecific nuclear/nucleolar staining was observed in some cells, especially in the hibernoma (B). Except for one lipoma (Table 1), all benign and malignant tumors expressed leptin.

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FIGURE 2.

Expression of leptin and the long form of the leptin receptor (OB-Rb) in lipomas and liposarcomas by RT-PCR. Top panel, leptin; middle panel, leptin receptor (OB-Rb), bottom panel, hypoxanthine phosphoribosyl transferase (HPRT) used to check the quality of the mRNA. Lanes 1 through 5, lipomas (Cases 6 to 10). Lanes 6–10, liposarcomas (Cases 6, 7, 9, 15, and 16). Lane 11, normal adipose tissue used as a positive control. Lane 12, negative RT control. The lower portion of each panel shows the Southern hybridization results using internal probes for each primer set as previously reported (27). The sizes of the amplified products are shown on the right.

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FIGURE 3.

In situ hybridization showed leptin mRNA and leptin receptor mRNA in all lipomas, hibernomas, and liposarcomas. Leptin mRNA expression in lipoma (A) and hibernoma (B). Leptin receptor mRNA expression in well-differentiated (C) and myxoid liposarcoma (D). Nonspecific nuclear/nucleolar staining for leptin and OB-Rb mRNA was seen in some cells of liposarcoma and hibernoma (blue, positive signal; red, nuclear fast red counterstain).

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FIGURE 4.

Western blotting to localize leptin in a lipoma and a liposarcoma. Top panel (lane 1), lipoma; (lane 2), liposarcoma; (lane 3), 50 ng of purified leptin protein used as a positive control; 50 mug of protein was used in lanes 1 and 2. The bottom panel shows the result of blotting with beta-actin to normalize for equal loading of the gel. The molecular sizes of the bands are indicated on the right.

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TABLE 1 - Clinicopathologic Features and Leptin and Leptin Receptor Expression in Adipose Tissue Tumors - Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, please contact help@nature.com or the author

TABLE 1 - Clinicopathologic Features and Leptin and Leptin Receptor Expression in Adipose Tissue Tumors.

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