Modern Pathology

FIGURE 4

FROM:

Leptin and Leptin Receptor mRNA are Widely Expressed in Tumors of Adipocytic Differentiation

Andre M Oliveira, Antonio G Nascimento and Ricardo V Lloyd

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FIGURE 4.

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Western blotting to localize leptin in a lipoma and a liposarcoma. Top panel (lane 1), lipoma; (lane 2), liposarcoma; (lane 3), 50 ng of purified leptin protein used as a positive control; 50 mug of protein was used in lanes 1 and 2. The bottom panel shows the result of blotting with beta-actin to normalize for equal loading of the gel. The molecular sizes of the bands are indicated on the right.

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