Modern Pathology

FIGURE 2

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Leptin and Leptin Receptor mRNA are Widely Expressed in Tumors of Adipocytic Differentiation

Andre M Oliveira, Antonio G Nascimento and Ricardo V Lloyd

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FIGURE 2.

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Expression of leptin and the long form of the leptin receptor (OB-Rb) in lipomas and liposarcomas by RT-PCR. Top panel, leptin; middle panel, leptin receptor (OB-Rb), bottom panel, hypoxanthine phosphoribosyl transferase (HPRT) used to check the quality of the mRNA. Lanes 1 through 5, lipomas (Cases 6 to 10). Lanes 6–10, liposarcomas (Cases 6, 7, 9, 15, and 16). Lane 11, normal adipose tissue used as a positive control. Lane 12, negative RT control. The lower portion of each panel shows the Southern hybridization results using internal probes for each primer set as previously reported (27). The sizes of the amplified products are shown on the right.

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