Modern Pathology



Detection of the ETV6-NTRK3 Chimeric RNA of Infantile Fibrosarcoma/Cellular Congenital Mesoblastic Nephroma in Paraffin-Embedded Tissue: Application to Challenging Pediatric Renal Stromal Tumors

Pedram Argani, Michael Fritsch, ShriHari S Kadkol, Amy Schuster, J Bruce Beckwith and Elizabeth J Perlman



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Detection of ETV6-NTRK3 fusion in congenital mesoblastic nephroma. Top, agarose gel electrophoresis of PGK RT-PCR products reveals the presence of a 263 base pair band signifying amplifiable RNA in all cases except for Case 7. The size of selected bands in the molecular weight marker lane M are indicated on the left. Middle, agarose gel electrophoresis of reverse transcriptase polymerase chain reaction products obtained with ETV6-NTRK3 primers on the same RNAs. Cases 1 to 4 are cellular congenital mesoblastic nephromas (CMNs), with Case 3 representing the cellular CMN with prominent rhabdoid features illustrated in Figure 2. Case 5 is a mixed CMN for which the block studied showed only classic morphology, whereas both Cases 6 and 7 are classic CMNs. Bottom, Southern blot of the agarose gel in the middle panel hybridized with the ETV6-NTRK3 fusion probe confirms the positivity of Cases 1 to 4 and the negativity of Cases 5 to 7. Note that Case 7 is not truly evaluable because the phosphoglycerate kinase gene result was negative.