Article

Mucosal Immunology (2015) 8, 1083–1098; doi:10.1038/mi.2014.135; published online 11 February 2015

Phenotype and function of nasal dendritic cells

H Lee1,2, D Ruane1,2,10, K Law2,3,10, Y Ho4, A Garg1, A Rahman2,5, D Esterházy6, C Cheong7, E Goljo4, A G Sikora8, D Mucida6, B K Chen2,3, S Govindraj4, G Breton9 and S Mehandru1,2

  1. 1Division of Gastroenterology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA
  2. 2Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA
  3. 3Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA
  4. 4Department of Otorhinolaryngology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
  5. 5Flow Cytometry Shared Resource Facility, Icahn School of Medicine, New York, New York, USA
  6. 6Laboratory of Mucosal Immunology, Rockefeller University, New York, New York, USA
  7. 7Laboratory of Cellular Immunology and Physiology, Institut de Recherches Cliniques de Montréal and Department of Microbiology and Immunology, Université de Montréal, Montreal, Quebec, Canada
  8. 8Bobby R. Alford Department of Otolaryngology-Head and Neck Surgery, Baylor College of Medicine, Houston, Texas, USA
  9. 9Laboratory of Molecular Immunology, Rockefeller University, New York, New York, USA

Correspondence: S Mehandru, Saurabh.mehandru@mssm.edu

10These authors contributed equally to this work.

Received 8 September 2014; Accepted 25 December 2014
Advance online publication 11 February 2015

Top

Abstract

Intranasal (i.n.) vaccination generates immunity across local, regional, and distant sites. However, nasal dendritic cells (DCs), pivotal for the induction of i.n. vaccine-induced immune responses, have not been studied in detail. Here, by using a variety of parameters, we define nasal DCs in mice and humans. Distinct subsets of “classical” DCs, dependent on the transcription factor zbtb46 were identified in the murine nose. The murine nasal DCs were Fms-related tyrosine 3 kinase ligand responsive and displayed unique phenotypic and functional characteristics, including the ability to present antigen, induce an allogeneic T-cell response, and migrate in response to lipopolysaccharide or live bacterial pathogens. Importantly, in a cohort of human volunteers, BDCA-1+ DCs were observed to be the dominant nasal DC population at steady state. During chronic inflammation, the frequency of both BDCA-1+ and BDCA-3hi DCs was reduced in the nasal tissue, associating the loss of these immune sentinels with chronic nasal inflammation. The present study is the first detailed description of the phenotypic, ontogenetic, and functional properties of nasal DCs, and will inform the design of preventative immunization strategies as well as therapeutic modalities against chronic rhinosinusitis.

Top

Introduction

Dendritic cells (DCs) have a pivotal role in immune homeostasis, marshaling immunity against pathogens, while maintaining tolerance to commensals. The nasal associated lymphoid tissue (NALT) serves as a site for the induction of nasal,1, 2 pharyngeal,3 respiratory,4 oral,2 gastrointestinal,5, 6 genitourinary,7, 8 and systemic9 immunity. Therefore, it follows that the nose should contain a significant population of DCs capable of priming and disseminating immune responses. However, nasal DCs have not yet been characterized and are the focus of the present study.

The murine NALT consist of paired lymphoid structures, resting on the nasal surface of the soft palate on either side of the nasal septum.10, 11, 12 The NALT is covered by follicle-associated epithelium13 interspersed with M cells,14, 15, 16 goblet cells,17, 18 and intra-epithelial lymphocytes.19 Within the NALT, B cells predominate over T cells at steady state,7, 20, 21 with IgD+ and IgM+ B cells constitutively present and IgA+ B cells readily inducible following intranasal (i.n.) vaccination.8, 22 Among T cells, Th0 CD4+ T cells, CD8+αα, CD8+αβ, and CD8+γδ T cells have been described in the NALT, with CD4+ T cells predominating over CD8+ T cells at steady state.19, 23, 24 Although structural similarities between the NALT and Peyer’s patches are noted,21, 23 important differences exist with regards to development13 and lymphocyte homing.25, 26

DCs initiate the adaptive immune response.27 Since the initial description of DCs as “CD11c+ cells”, we have come to recognize the complexity and lineage diversity of DCs in various tissues.28, 29 Among the nasal DCs, although studies have alluded to CD11c+ cells in the NALT,20, 30 there are no descriptions of DC phenotype, subsets, or function.

Here, by using a variety of assays, we have defined the morphology, phenotype, ontogeny, and function of mouse nasal DCs. In doing so, we demonstrate the complexity of nasal DCs and their behavior in the face of inflammatory stimuli such as lipopolysaccharide (LPS). In addition, we have examined putative DC subsets in surgically resected human nasopharyngeal tissue from normal volunteers as well as patients with chronic rhinosinusitis (CRS). The data presented herein are the first description of nasal DCs and will inform the design of novel i.n. vaccines for prevention and treatment of diseases.

Top

Results

Morphology of nasal DCs in mice

The NALT were identified on the nasal surface of the palate, between the incisors and first molar teeth as tridimensional, oval structures on hematoxylin and eosin staining. Higher magnification (×400) revealed aggregates of mononuclear cells, populating the NALT (Figure 1a). To further study these mononuclear cells, nasal cryosections were stained with combinations of fluorescent antibodies identifying putative CD11c+ DCs, CD3+ T cells, and B220+ B cells. Respective isotype controls confirmed the specificity of staining (data not shown). Distinct T and B areas were noted in the NALT as reported previously.30 Notably, DCs were predominantly seen in the T-cell areas or in the periphery of the NALT (Figure 1b). Three-dimensional imaging using two-photon microscopy revealed a high density of CD11c-eYFP+ cells uniformly distributed on the NALT surface (Figure 1c). The majority of CD11c-eYFP+ cells were embedded among collagen fibers visualized by second harmonic generation and displayed numerous dendrites (Supplementary Figure S1 online). However, on deeper imaging through the NALT, CD11c-eYFP+ cells reduced in frequency and showed a less ruffled and condensed morphology (data not shown). To further study the peripherally distributed CD11c+ cells, nasal sections were double-labeled with anti-mouse keratin 18 and CD11c. A dense network of CD11c+ cells was found subjacent to the epithelium (Figure 1d). Fortuitously, in some of the sections we detected that nasal DCs were extending dendrites across the nasal epithelium, into the lumen of the nose (Figure 1d). As extension of DC dendrites was observed infrequently, we are unable to quantify this in the present study. We next studied the association of nasal CD11c+ cells with blood vessels (anti-PECAM-1) and lymphatic channels (anti-LYVE-1), and found nasal DCs clustering with lymphatic vessels in the posterior aspects of the NALT (Figure 1e) as well as the blood vessels distributed throughout the NALT (Figure 1f). Thus, a high density of putative DCs, positioned to sample the airway antigens, was noted within the murine nose.

Figure 1.
Figure 1 - Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, please contact help@nature.com or the author

Dendritic cells (DCs) in murine nasal-associated lymphoid tissue (NALT). (a) Hematoxylin and eosin (H&E) staining was used to define the morphology of murine NALT (indicated with blue arrowheads) at × 200 original magnification (top) and at × 400 original magnification (bottom). (b) Immunofluorescent staining to define the relationship of NALT-associated DCs with T and B cells. Confocal micrograph of the entire longitudinal section of NALT is depicted here by combining individual images in Adobe Photoshop CC. CD3+ T cells (green), B220+ B cells (blue), and CD11c+ cells (red) are shown. Inset highlights a magnified area demonstrating that the CD11c+ cells are predominantly located in the T cell zones and in the periphery of the NALT. Each figure is representative of at least five experiments. Bars = 50μm. (c) Three-dimensional reconstruction from two-photon microscopy data showing spatial distribution of nasal CD11c+ cells (green) from enhanced yellow fluorescent protein (eYFP) transgenic mice and collagen second harmonic generation (blue). Raw fluorescent image (top) and reconstructed image (bottom) are depicted. (df) Immunofluorescent staining to define the relationship of NALT DCs with the nasal epithelium, blood vessels, and lymphatic channels. (d) The relationship of CD11c+ cells (red) with the cytokeratin 18+ nasal epithelial cells (green) and B220+ B cells (blue). White arrowhead shows the extension of CD11c+ dendrites across the epithelium into the nasal lumen. (e) The relationship of CD11c+ cells (red) with LYVE1+ lymphatic channels (green) and B220+ B cells (blue). (f) The proximity of CD11c+ cells (red) to PECAM1+ blood vessels (green) and B220+ B cells (blue). In d, e, and f, confocal micrographs of the entire longitudinal section of NALT are depicted by combining individual images in Adobe Photoshop CC. A, anterior; P, posterior. Each figure is representative of at least three experiments. Bars = 50μm.

Full figure and legend (430K)Download PowerPoint slide (563 KB)

Phenotypic characterization of murine nasal DCs identifies distinct subsets of myeloid DCs

Using multi-parameter flow cytometry, we first examined DCs from the entire nose and gated on live, CD45+, lymphocyte lineage-negative, CD11c+MHCII+ cells isolated from mechanically disrupted and collagenase-digested whole nasal tissue. Three distinct populations of putative DCs could be identified using anti-CD103 and anti-CD11b antibodies: CD103+CD11b cells, CD11b+CD103 cells, and CD103+CD11b+ double-positive cells (Figure 2a), with CD11b+CD103 cells being the predominant population. However, CD11b+CD103 cells are heterogeneous and comprise both classical DCs (cDCs) and macrophages (Mφ). CD64 (FcγR1) has been shown to recognize Mφ selectively7, 26, 31, 32 and can help distinguish cDCs from Mφ within CD103CD11b+ cells.

Figure 2.
Figure 2 - Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, please contact help@nature.com or the author

Phenotypic characterization of nasal dendritic cells (DCs). (a,b,c) Single-cell suspension of nasal cells was obtained by mechanical disruption and collagenase digestion of nasal tissue. Multiparameter flow cytometry was used to identify DC subsets within the murine nose. (a) The gating strategy used to delineate myeloid DC (mDC) subsets based on the expression of CD103, CD11b, and CD64 on live, CD45+lineageCD11c+MHCII+ cells. For identification of plasmacytoid DCs (pDCs), MHCII cells were defined on the basis of CD11c and PDCA-1 staining. PDCA-1hiCD11c+B220+ cells were deemed putative pDCs. (b) Absolute number of all DC subsets including pDCs. Error bars=s.d. (c) Identification of nasal associated lymphoid tissue (NALT) and non-NALT DCs separately. (d) eYFP+ cells in non-NALT mucosa. Bars = 50μm (left) and 25μm (right). (e) Detailed phenotypic characterization of the nasal antigen presenting cell (APC) subsets using a panel of monoclonal antibodies. (f) Wright–Geimsa stains were used to define the cellular morphology of putative nasal DCs and nasal Mφ. Bars = 10μm. (g) CD11c+langerin+DCs were analyzed by immunofluorescence using confocal microscope where langerin+ cells are depicted in green, CD11c+ cells in red, and CD3+ cells in blue. White arrowheads indicate the cells where co-localization of langerin with CD11c was observed. Bars = 50μm.

Full figure and legend (347K)Download PowerPoint slide (478 KB)

We initially tested the expression of CD64 and heat-stable antigen or CD24, an important co-stimulatory molecule for the induction of T-helper cells,33 on CD11c+MHCII+ gated CD103CD11b+F4/80+ cells. As shown in Supplementary Figure S2, the CD103CD11b+F4/80+ cells contained both CD24+ and CD24 cells. Further analyses revealed that the CD64+ cells were contained within the CD11b+F4/80+ cells, while both CD11b+F4/80 and CD103+CD11b cells lacked CD64 expression (data not shown). These data indicated CD11c+MHCII+ gated CD103CD11b+ cells within the nose contain both cDC and Mφ, which could be defined as CD64F4/80 cDC and CD64+F4/80+ Mφ (Figure 2a). In addition, plasmacytoid DCs were identified among MHCII nasal cells as PDCA-1hiCD11c+B220+ cells (Figure 2a). The total number of cells contained within each of the DC subsets are quantified in Figure 2b. Next, we examined DCs in the inductive (NALT) and effector (nasal passages) sites of nose separately. As expected, the density of putative DCs was significantly greater in the NALT tissue compared with that in the nasal passages. Interestingly, the CD103+CD11b+ cells were predominantly found in the nasal passages, while both the NALT and non-NALT tissue contained CD103+CD11b and CD103CD11b+ cells (Figure 2c). Confocal images from CD11c-eYFP mice confirmed that eYFP+ DCs were present in non-NALT nasal mucosa (Figure 2d).

A detailed phenotypic assessment of each of the putative DC subsets was performed next (Figure 2e). Interestingly, all subsets of nasal CD11c+MHCII+ cells lacked CD8α. Although both the CD103+CD11b and CD103+CD11b+ cells were CD24hi, among the CD11b+ cells the CD64+F4/80+ and CD64F4/80+ subsets were predominantly (~90% cells) CD24. In contrast, CD64F4/80 cells contained equal numbers of CD24+ and CD24 cells. CD115 was not expressed by any of the nasal CD11c+MHCII+ subsets. Further, the CD103+CD11b cells were negative for macrophage mannose receptor CD206, while CD11b+CD103+ cells were CD206int. Among the CD11b subsets, CD64+F4/80+ cells were CD206hi, both CD64F4/80+ and CD64F/40 cells were CD206lo to CD206int. CD103+CD11b, and CD103+CD11b+ cells were EpCAMhi, while the CD11b+ subsets contained both EpCAMhi and EpCAMlo cells. Finally and expectedly, LPS receptor, CD14, was expressed by the CD11b+CD64+F4/80+ cells as well as CD11b+CD64F4/80+ cells, while the CD11b+CD64F4/80 cells contained both CD14+ and CD14 cells. In contrast, CD103+CD11b cells were CD14 negative.

Collectively, these data show that among the CD11c+MHCII+ nasal cells, we could identify five distinct populations of antigen presenting cells: (a) CD103+CD11b cells expressing phenotypic markers associated with CD103+ DC,34 (b) the CD103CD11b+ cells, a heterogeneous population, comprising three distinct subsets—(i) CD64+F4/80+ cells that had the phenotypic markers associated with Mφ, (ii) CD64F4/80 cells that had markers associated with CD11b+ cDCs, and (iii) CD64F4/80+ cells that appeared to have both Mφ- and cDC-like cells. (c) Finally, the nose also contained CD103+CD11b+ cells that shared phenotypic characteristics of both CD103+ DC and CD11b+ DC.

We next identified the morphological characteristics of putative nasal DCs and nasal Mφ. Wright–Geimsa staining of cytospins of the fluorescence-activated cell-sorted nasal DCs (defined as MHCII+CD11c+F4/80CD64 cells based on the criteria defined above) as well as putative nasal Mφ (defined as MHCII+CD11c+F4/80+CD64+ cells) revealed clear morphological differences. Although nasal DCs had abundant cytoplasm and “dendrites,” nasal Mφ showed an eccentric, reniform nucleus with intracellular granules and lack of significant membrane extensions or dendrites. Further, as the CD11c+MHCII+CD64F4/80+ cells were a major population (Figure 2a), we examined them separately as well. Although a majority of CD11c+MHCII+CD64F4/80+ cells displayed the morphology of Mφ with a granulated cytoplasm and reniform nucleus, cells with cytoplasmic extensions and less vacuolated cytoplasm, cells resembling DCs could also be seen (Figure 2f). Thus, the CD11c+MHCII+CD64F4/80+ cells were a mixed population comprising both Mφ and DCs.

Finally, we wanted to determine the location CD103+CD11b cells within the NALT. As CD103+CD11b cells exclusively expressed langerin, we used a combination of antibodies specific to CD11c, langerin, and CD103, to denote putative CD103+ DCs in the NALT. The frequency of langerin+ cells that co-labeled with CD11c was rare. When found, the langerin+CD11c+ cells were mostly located toward the periphery of the NALT (Figure 2g), consistent with the expression of epithelial cell adhesion molecule (EpCAM) by these cells, as noted by flow cytometry.

FLT3 ligand expands CD103+CD11b nasal DCs and CD103CD11b+F4/80CD64 nasal DCs

Fms-related tyrosine 3 kinase (FLT3) ligand, a key cytokine in DC differentiation,35 has also been used as a potent adjuvant in nasally administered vaccines.29, 33, 36 To determine the effect of FLT3 ligand on nasal DCs, we implanted mice with 5−7 × 106 B16-FLT3L melanoma (B16-FLT3L) cells37 and used mice implanted with B16 melanoma (B16) cells or wild-type (WT) mice as controls. Nasal CD11c+MHCII+ cells were increased 12-fold in the B16-FLT3L mice compared with WT or B16 mice (Figure 3a). Among the CD11c+MHCII+ gated cells, the frequency of all nasal DC subsets was increased in B16-FLT3L mice, with CD103+CD11b cells and CD103CD11b+ cells showing the most appreciable increase in numbers (Figures 3a,b). Among the CD103CD11b+ gated cells, a dramatic expansion in CD11b+CD64 cells was seen (both CD64F4/80+ and CD64F4/80 subsets). This further supported that CD64 is useful to distinguish between cDCs and Mφ among the nasal CD103CD11b+ cells. Immunofluorescent staining of the NALT further confirmed the dramatic increase in CD11c+ cells seen in the B16-FLT3L mice but not the B16 melanoma-implanted mice (Figure 3c).

Figure 3.
Figure 3 - Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, please contact help@nature.com or the author

Identification of Fms-related tyrosine kinase 3 ligand (FLT3L) responsive nasal dendritic cell (DC) subsets. C57BL/6 wild-type (WT) mice were implanted with 5–7 × 106 B16-FLT3 ligand melanoma cells (B16-FLT3L) or B16 melanoma cells (B16), and the nasal DC subsets were analyzed by multiparameter flow cytometry (a,b) or immunofluorescence (c) after 10–14 days of tumor implantation. (a) Representative flow cytometry panels comparing the expansion of putative nasal DCs in B16 or B16-FLT3L mice. (b) Cumulative data from three individual experiments Error bars=s.d. *P<0.05, **P<0.01; ns,not significant. (c) Nasal cryosections from mice implanted with B16-FLT3L or B16 melanoma cells were stained for CD3 (green), CD11c (red), and B220 (blue). Bars = 50μm. The entire nasal associated lymphoid tissue (NALT) is depicted here by combining individual images in Adobe Photoshop CC.

Full figure and legend (250K)Download PowerPoint slide (522 KB)

In order to further confirm these data, we performed additional experiments comparing the nasal populations of B16 and B16-FLT3L mice using mass cytometry (CyTOF), a novel, potentially paradigm-changing technology that combines the principles of flow cytometry with mass spectrometry, thereby allowing the simultaneous examination of significantly more variables per cell than is possible by conventional flow cytometry.38 We analyzed nasal cell suspensions using a 20-parameter CyTOF panel focused on DC- and Mφ-associated markers (Supplementary Table S4). We clustered DNA+CD45+ events into a minimum-spanning tree of phenotypically related nodes using spanning-tree progression analysis of density-normalized events24 (Figure 4). Node clusters corresponding to definable cell populations were manually annotated on the basis of canonical marker expression, while preserving visualization of unidentified clusters within the data set. The spanning-tree progression analysis of density-normalized event trees showed a clear and striking expansion of the CD11c-expressing DC populations following FLT3L treatment (Figure 4a). The expanding populations also expressed high levels of MHCII, and further delineation of the underlying phenotypic heterogeneity revealed a distribution of CD24, CD103, CD117, and CD11b expression among the expanding subsets, whereas there was no expansion in the CD64-expressing populations. For the sake of representation, only FLT3L-treated mice, focusing on putative cDC and Mφ, are shown in Figure 4b. These data are summarized in Figure 4c, comparing the fold-change differences in the annotated cell populations as a percentage of total CD45+ cells between the B16-FLT3L and B16 mice. Fold-change differences showed a dramatic expansion in CD24hiCD103lo (73-fold) nasal cells in the B16-FLT3L-treated mice. Notably, these cells were MHCIIhiCD11c+CD11b+, thus confirming the flow cytometry data. In addition, FLT3L treatment resulted in expansion in CD24hiCD103hi cells (40-fold).

Figure 4.
Figure 4 - Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, please contact help@nature.com or the author

CYTOF plots comparing nasal populations in B16 melanoma and Fms-related tyrosine kinase 3 ligand (FLT3L)-B16 melanoma mice. (a) Spanning-tree progression analysis of density-normalized event (SPADE) plots showing the expression of CD11c in nasal mononuclear cell populations as indicated. (b) Macrophage and dendritic cell (DC) focused on spade trees showing the expression of the indicated markers in FLT3L-treated mice. The size of each node represents the number of cells, and the strength of expression of the respective marker is indicated by the color as specified in the legend. (c) Summary of the CYTOF data showing fold changes in the respective populations, comparing nasal populations in B16 and B16-FLT3L mice.

Full figure and legend (321K)Download PowerPoint slide (432 KB)

Together, these data show that the nasal cDCs include a FLT3L responsive, minor CD103+ population and a major CD11b+ population that is CD24+ and CD64. In addition, within the CD11b+ population, CD64+ cells are FLT3L unresponsive, Mφ-like cells. Ontogenic and functional data shown below further confirm these results.

Zbtb46-DTR and LysmCre x Csf1rLsL−DTR identify cDC and Mφ, respectively, in the nose

Within the hematopoietic compartment, the zinc finger transcription factor Zbtb46 is specifically expressed by cDC as opposed to the overlapping expression of CD11c on DCs, Mφ, and activated monocytes.39, 40 Moreover, a recently described LysmCre x Csf1rLsL−DTR (MMDTR) mouse model identifies monocytes and Mφ.41 Using a cell niche depletion approach, we defined cDC and Mφ in the nose using zDCDTR-WT (referred to as zDCDTR), LysmCre x Csf1rLsL−DTR-WT (MMDTR), and WT–WT (WT) bone marrow chimeric mice. Nasal mononuclear cells were examined 24h post administration of diphtheria toxin (DT). In the zDCDTR mice, among the CD11c+MHCII+ gated cells, CD103+CD11b cells (called CD103+ DC henceforth) were depleted significantly and CD103+CD11b+ cells (called CD103+11b+ DC) tended to reduce as well. In contrast, in the MMDTR mice CD103+ DC, CD103+CD11b+ DCs were not depleted, while the CD103CD11b+ cells were significantly depleted. (Figure 5a,b). On further examining the CD103CD11b+ cells, we found that in the zDCDTR mice CD11b+CD64F4/80 cells were significantly depleted, while they were unaffected in the MMDTR mice, confirming these cells to be cDCs. In addition, the CD11b+CD64+F4/80+ cells and CD11b+CD64F4/80+ cells were significantly depleted in the MMDTR mice, confirming these cells to be derived from the macrophage/monocytic lineage. Thus, based on ontogeny, we could define CD103+CD11b and CD11b+CD64F4/80 cells as cDC in the nose.

Figure 5.
Figure 5 - Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, please contact help@nature.com or the author

Identification of “classical dendritic cells (DCs)” and Mφ in the murine nose. zDCDTR (Zbtb46-DTR) Tg or MMDTR (LysmCre x Csf1rLsL−DTR) or wild-type (WT) bone marrow chimeric mice were injected intraperitoneally (i.p.) with 500ng diphtheria toxin (DT) and live, CD45+ nasal cells were analyzed for expression of CD11c, MHCII, CD103, CD11b, F4/80, and CD64 1-day post DT administration. Numbers represent the percentage of cells in the indicated gate. (a) Representative flow cytometry plots comparing the percentage of nasal antigen presenting cells between WT, zDCDTR, and MMDTR mice. (b) Cumulative data from three individual experiments is depicted here. Error bars=s.d. *P<0.05, **P<0.01, ***P<0.001.

Full figure and legend (236K)Download PowerPoint slide (447 KB)

Nasal DCs present soluble antigen to OVA-transgenic T cells and induce MLR to allogeneic T cells

Having examined the anatomical, phenotypic, morphological, and ontogenetic features of nasal “DCs,” we sought to use functional assays to confirm that these cells were indeed bona fide DCs. Antigen presentation to syngeneic T cells and mixed leukocyte reaction (MLR) to allogeneic T cells is among the most distinctive characteristics of DCs and distinguishes them from the closely related Mφ.42 In order to obtain sufficient numbers of cells to run functional assays and guided by the results of our FLT3L experiments, mice were implanted with B16-FLT3L melanoma cells. Nasal DCs (defined as CD11c+MHCII+F4/80CD64 cells), nasal Mφ (defined as CD11c+MHCII+F4/80+ CD64+ cells), splenic DCs (defined as LinCD11chiCD11bint cells, used as a positive control), and splenic B cells (negative control) were obtained by flow cytometric sorting. Notably, among the CD11c+MHCII+ cells, the CD103+ and CD11b+ subsets could not be used for co-cultures separately due to cell number limitations.

For the antigen-presentation assays, respective cell populations were co-cultured with carboxyfluorescein succinimidyl ester (CFSE)-labeled ovalbumin (OVA)-transgenic (OT-II) CD4+ T cells in the presence OVA. To exclude the possibility that the OVA protein was degraded into peptides, which then bound spontaneously to MHCII molecules on T cells and induced T-cell proliferation, we also included a control wherein OT-II cells were cultured in the presence of OVA alone, without DCs. The frequency of proliferating, Vα2+CD4+CFSElo T cells was quantified after 5 days of culture. A clear difference in antigen-presenting ability was noted between nasal DCs and Mφ. Although nasal DCs, analogous to splenic DCs induced OT-II proliferation in the presence of OVA, nasal Mφ analogous to splenic B cells did not (Figures 6a,b). OT-II cells cultured in the presence of OVA alone did not proliferate (data not shown). Nasal DCs showed poor ability to cross-present to CD8+ T cells. This probably reflects the distribution of DCs in the nose, i.e., cross-presenting, CD8-priming, CD103+ DCs are a minority population, whereas the CD4-priming CD11b+ DCs are the dominant population.

Figure 6.
Figure 6 - Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, please contact help@nature.com or the author

Nasal dendritic cells (DCs) present antigen to CD4+ T cells and induce proliferation of allogeneic T cells in a mixed leukocyte reaction (MLR). (a) Nasal DCs from C57BL/6 mice were expanded with B16-FLT3L cells for 10–14 days before sorting. Nasal antigen presenting cells were sorted into two subsets: CD11c+MHCII+ F4/80CD64 cells indicated as nose DCs and CD11c+MHCII+F4/80+CD64+ cells indicated as nose Mφ. Sorted cells were cultured in the indicated DC:T-cell ratio for 5 days with carboxyfluorescein succinimidyl ester (CFSE)-labeled splenic OT-II cells (left) or BALB/c splenic T cells (right). Spleen DCs were used as the positive control and spleen B cells were used as the negative control. Representative flow cytometry plots comparing the proliferation of Vα2+CD4+ T cells (left) or allogeneic T cells (right) by nasal DCs and Mφ. Cumulative data from one of three experiments is shown here. Error bars=s.d. (b) Quantification of the number of proliferated (CFSElo) OT-II T cells (left) or Balb/c T cells (right). Cumulative data from one of three experiments is shown here. Error bars=s.d.

Full figure and legend (168K)Download PowerPoint slide (409 KB)

For MLR assays, the nasal DCs, nasal Mφ, splenic DCs, and splenic B cells were cultured with CFSE-labeled BALB/c splenic T cells (as the allogeneic responders). Nasal DCs effectively stimulated allogeneic T cells similar to splenic DCs, while nasal Mφ and splenic B cells were poor at inducing MLR (Figures 6a,b). These data highlight the functional differences between CD11c+MHCII+F4/80CD64 cells and CD11c+MHCII+F4/80+ CD64+ cells, and confirm the former to be bona fide DCs within the nose.

Nasal DCs migrate in response to LPS

Migratory behavior is a hallmark of DCs.43 To investigate this as yet another criteria to define nasal DCs, mice were administered OVA-Alexa 488 in combination with LPS delivered intranasally. The nose and draining lymph nodes (LNs) were examined at serial time points (1, 6, 12, and 24h post administration). Phosphate-buffered saline (PBS)-administered mice were used as controls at each time point. In the nose, we assessed the uptake of OVA by live, CD45+ cells and found that a minority (~3%) of OVA+ nasal cells were CD11c+MHCII+, with peak labeling seen at 6h and declining by 24h. Further examination of the OVA+CD11c+MHCII+ cells revealed them to be predominantly CD11b+ (~99%). Among the CD11b+ cells, ~80% of OVA+ cells were CD64 bona fide DCs. Interestingly, a majority of the total OVA+ nasal cells were CD11cMHCIIlo/intly6Chily6GhiSSCint granulocytes. Peak uptake of OVA by granulocytes occurred at 6h and declined over 24h (although persisting well above baseline at 24h). In addition, a small population of OVA+CD11cMHCIIlo/intly6Chily6GSSClo monocytes appeared at 6h and persisted over 24h in the nose (Figures 7a,b).

Figure 7.
Figure 7 - Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, please contact help@nature.com or the author

Migration of nasal dendritic cells (DCs) to cervical draining lymph nodes (LNs). (a,b) Targeting of nasal DCs and neutrophils following intranasal administration of OVA-Alexa 488 and migration of OVA+ DCs to the cervical LNs (cLNs). (a,b) At least three wild-type (WT) mice per time point were administered either OVA Alexa 488 plus lipopolysaccharide (LPS) or were given phosphate-buffered saline (PBS) intranasally and analyzed at 1, 6, 12, and 24h. (a, top) Representative flow cytometric analysis of OVA+ cells. Both nasal DCs (CD11c+MHCII+CD11b+CD64 cells) and neutrophils (CD11cMHCIILy6ChiLy6Ghi cells) are shown; (bottom) representative flow cytometric analysis of OVA+ migratory DCs (CD11c+MHCIIhi cells) in the cLN at respective time points. (b) Cumulative data from three individual experiments is quantified here. Error bars=s.d. (c) Uptake of S. typhimurium by CD11c-eYFP. cells derived from the murine nose and cervical LN is compared. (d) Three-dimensional reconstruction from two-photon microscopy data showed dramatic change in spatial distribution of CD11c-eYFP+ DCs (green) and mCherry-expressing Salmonella (red) between nasal associated lymphoid tissue (NALT) and cLNs. PBS and pertussis toxin (PTX)-administered mice are compared at 5 min, 35 min, and 12 h using two-photon microscopy. Second-harmonics signals from collagen are in blue. Bars = 30μm. (e) Numbers of mCherry+ cells in NALT (top) and cLN (bottom) were quantified using the Imaris software (Bitplane). Each figure is representative of at least three experiments. Error bars=s.d.; original magnification= × 400.

Full figure and legend (428K)Download PowerPoint slide (456 KB)

In parallel to the nose assessments, we also examined the draining LNs—both cervical LN (cLN) and mediastinal LN for migratory DCs (live, OVA+linMHCIIhiCD11c+ cells). As shown in Figure 7a and quantified in Figure 7b, a small population of OVA+ migratory DCs could be detected in the cLN at 6h, with a progressive and striking increase at 24h. Further analyses of these migratory DCs within the cLN revealed them to be predominantly CD11b+CD64, with a small number of CD103+CD11b and CD103+CD11b+ DCs as well, reflecting the numeric distribution of these DC subsets in the nose. As much as 40% of the OVA+ cells in the cLN at 24h were CD11cint/MHC-IIint/lo (Figure 7a). However, we did not include granulocyte-specific markers in the LN panel. Therefore, we were unable to confirm whether OVA+ neutrophils also migrate into the LN. Interestingly, we did not detect OVA+ migratory DCs within the mediastinal LN even at 24h (data not shown). Thus, we were able to demonstrate clear uptake of a fluorescent antigen by nasal DCs and their subsequent migration to the draining LN.

Next, to examine the response of nasal DCs to Gram-negative pathogens, Salmonella enterica serovar Typhimurium constitutively expressing mCherry (Salmonella-mCherry) was administered intranasally to CD11c-eYFP transgenic mice. To compare DC-associated or DC-independent clearance of Salmonella from the nose, we co-administered either PBS or pertussis toxin (PTX). The latter is a known inhibitor of DC motility.34 Using two-photon microscopy, Salmonella-mCherry were seen in the nasal cavity as early as 5min after application, distributed within the nasal lumen (Figure 7d) as well as occasionally internalized within CD11c+ nasal cells (Figure 7c). With surprising kinetics, 35min post administration, in the PBS group, a (~50%) decline in mCherry+ cells was seen in the nose. In contrast, PTX-treated mice had a significantly greater number of mCherry+ cells compared with the nose of PBS-treated mice. This difference persisted at 12h (Figures 7d,e). Analogously, within the cLN, a striking increase in mCherry+ cells was seen in the PBS-treated but not PTX-treated mice at 35min and 12h. These data demonstrate a rapid flux of Salmonella into the draining LNs and highlights the rapid response of the antigen-presenting cells in the nose to a bacterial challenge, which is attenuated by PTX, an inhibitor of DC migration.

BDCA-1+ DCs form the predominant DC population in human nasopharyngeal tissue at steady state and are significantly reduced during chronic inflammation

To identify nasal DCs in humans, we analyzed nasopharyngeal and sinus tissues from 10 normal volunteers (a total of 10 tissue samples) and 10 patients with CRS (a total of 21 tissue samples; Supplementary Table S1). The CRS patients were further divided into patients with and without nasal polyps, as these two clinical entities show distinct phenotypes, with patients having nasal polyps being more refractory to treatment. The clinical parameters that were assessed included patient age, sex, tissue analyzed, indications for surgery, and a thorough list of the concomitant medications immediately proximal to the surgery. The mean age of normal volunteers was 52 years (range 25–82), the mean age of patients with CRS was 52 years (range 40–75), while the mean age of patients with CRS/nasal polyps was 50 years (range 33–61). Patients with CRS demonstrated greater use of steroids and antibiotics. We performed a detailed multicolor flow cytometric analysis, first gating on single cells, live cells, CD45+, and lymphocyte lineage-negative BDCA-1+ cells, and we also surface labeled for BDCA-2+ and BDCA-3+. Three distinct populations of human nasal DCs were identified as BDCA-1+ myeloid DCs, BDCA-2+ plasmacytoid DCs, and BDCA-3hi myeloid DCs (Figure 8a). We observed a significantly higher frequency of BDCA-1+ DCs rather than BDCA-3hi DCs in normal volunteers as well as in patients with CRS. More importantly, when compared with normal volunteers, patients with CRS displayed a significantly lower frequency of BDCA-1+ DCs (P<0.05) and a trend toward lower BDCA-3hi DCs (P=0.07). These differences were not seen on comparing normal volunteers and patient with CRS with nasal polyps (Figure 8b). In addition to the DC subsets described above, a detailed analysis of nasal mononuclear cells was performed. This included monocyte subsets, neutrophils, T cells, B cells, and NK cells. A decline in the BDCA-1+ and BDCA-3hi DCs was not accompanied by a corresponding increase in other inflammatory cells within the nose (Supplementary Figure S3).

Figure 8.
Figure 8 - Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, please contact help@nature.com or the author

Identification of dendritic cells (DCs) in human nasal mucosa. (a) Live CD45+ mononuclear CD3 CD20 CD335 CD66b CD14 cells, i.e., T-, B-, NK-, neutrophils-, and monocyte-excluded cells were analyzed for BDCA-1, -2, and -3 markers. Three DC populations are shown: BDCA-1+ myeloid DCs (left), BDCA-2+ plasmacytoid DCs (middle), and BDCA-3hi myeloid DCs (right). (b) Frequency of BDCA-1+ cells, BDCA-2+ cells, and BDCA-3hi cells as a percent of CD45+ mononuclear cells of normal, chronic rhinosinusitis (CRS), or CRS with nasal polys. Composite data from 20 donors are shown. *P<0.05.

Full figure and legend (104K)Download PowerPoint slide (312 KB)

Top

Discussion

Mucosal vaccination is considered to be superior to systemic vaccines for the generation of local and perhaps disseminated mucosal immune responses. Owing to its non-invasive nature and imminent antigen availability (most notably circumventing enzymatic degradation that precedes gastrointestinal mucosal absorption), i.n. antigen delivery appears to be the most feasible and clinically relevant of the various routes of mucosal vaccination and is supported by the rapid development of newer technologies to improve vaccine potency (chitosan derivatives)44 and safety (nanogels).45 In fact, a substantial research initiative funded by the Bill and Melinda Gates foundation focuses on the development of novel, needle-free mucosal vaccines.37 In spite of these advances, basic descriptions of DCs in the nasal mucosa are lacking.

The observation that nasally delivered vaccines can induce antigen-specific immunity across a wide variety of systemic and mucosal sites21 led us to hypothesize that the nasal tissue would contain a high density of DCs. In support of our hypothesis, we observed that a robust population of DCs patrols the nasal cavity, anatomically distributed in sub-epithelial, peri-vascular, and peri-lymphatic spaces, poised to respond to antigen. The latter was confirmed by the rapid kinetics of nasal DC mobilization following i.n. administration of LPS or pathogenic Gram-negative bacteria. Further, we observed nasal DCs to possess a high phenotypic diversity with distinct subsets contained within CD11c+MHCII+ cells. Specifically, CD11c+MHCII+ gated CD103+CD11b nasal cells and CD11b+CD64F4/80 nasal cells are dependent on the transcription factor zbtb46 and show a preferential expansion by the cytokine FLT3L, defining these cells as “classical DCs,” capable of inducing a mixed leucocyte reaction and antigen presentation. In addition, these cells demonstrate classical DC morphology43 and migrate in response to antigen. In contrast to the DCs, CD11b+CD64+F4/80+ cells in the nose did not expand with FLT3L or deplete with DT in the zbtb46-DTR mice. However, this population was significantly depleted in the MMDTR mice. Morphologically, these cells demonstrated a vacuolated cytoplasm with an eccentric, reniform nucleus. Functionally, the CD11b+CD64+F4/80+ cells neither presented antigen nor induced MLR. Based on these criteria, we confirm nasal CD11b+CD64+F4/80+ cells to be Mφ. Moreover, a third population among the CD11b+ cells was seen—CD11b+CD64F4/80+. Morphologically, this was a mixed population comprising both DCs (minority of the cells) and Mφ (majority of the cells). Accordingly, this population is FLT3L responsive on one hand, while being significantly depleted in the MMDTR mice on the other. Furthermore, although the CD11b+CD64F4/80+ cells are also depleted in the zDCDTR mice, likely due to the lower number of DCs contained within this heterogeneous subset, the depletion does not meet statistical thresholds. Unexpectedly, the murine nose also contains CD103+CD11b+ DCs, particularly in the non-NALT nasal tissue that co-express CD11b and CD103 similar to the intestinal lamina propria DC subsets46 and are partially zbtb46 dependent (perhaps reflecting their heterogeneous composition). Owing to the small number of these cells, we are unable to perform functional assays specific to this population. However, nasal CD103+CD11b+ cells share phenotypic characteristics of CD103 and CD11b DCs; they co-express CD24, CD206, and EpCAM, and appear to be intermediate for F4/80 and CD14, and thus share characteristics of both CD103+ DCs and CD11b+ DCs. Thus, we have identified five subsets of antigen presenting cells within the murine nose. CD103+CD11b, CD103+CD11b+, and CD11b+CD64F4/80 cells identified as cDCs, CD11b+CD64+F4/80+ cells identified as Mφ, and CD11b+CD64F4/80+ cells being a mixed population, comprising both cDCs and Mφ.

We also document BDCA-1+ and BDCA3hi myeloid DCs, as well as BDCA-2+ plasmacytoid DCs within the human nasopharyngeal tissue, and observe a decline in myeloid DCs to be associated with CRS. In light of these observations, we believe the present study to be the first comprehensive anatomic, phenotypic, ontogenic, and functional assessments of nasal DCs.

In addition to identifying distinct subsets of myeloid nasal DCs, we conclude that the CD11b+CD64F4/80 DCs comprise majority of nasal DCs. In contrast to the Peyer’s patches (considered as immune inductive sites for the intestines), which have a high frequency of CD103+ DCs, the NALT (considered as inductive immune sites for the respiratory tract) contains a low frequency of CD103+ DCs. The nasal CD103+ DCs are langerin+CD24+EpCAM+TLR3+, zbtb46 dependent and FLT3L responsive. Therefore, it would appear that the nasal cDCs are responsive to FLT3L, explaining and emphasizing the adjuvant role of FLT3L in nasally delivered vaccines.29, 33, 36, 47

To define the anatomic relationships of nasal DCs with other cells, we have used two- and three-dimensional imaging. Two-dimensional micrographs suggested a sub-epithelial location of nasal DCs, both within and extraneous to the NALT, in agreement with similar such observations.30 Further, a dense network of CD11chi cells, with a classical “dendritic” morphology was found by two-photon microscopy of the NALT with second harmonics, suggesting a lumen-oriented distribution of nasal DCs. In fact, we documented the extension of dendrites across the NALT epithelium, suggesting that nasal DCs sample the luminal contents across intact epithelium akin to the gut resident CX3CR1+ cells.25 In addition, we found nasal DCs to cluster around blood vessels and especially around the lymphatic channels, situated in the posterior aspects of the NALT, and speculate that in the absence of afferent lymphatics perivascular DCs are entering the NALT, while peri-lymphatic DCs are exiting it. Deeper areas within the NALT contained CD11c+ cells, albeit with a compact, rounded (as opposed to dendritic) appearance on two-photon imaging, perhaps suggesting that as the DCs contacted antigen, they became activated, lost their dendrites, and migrated deeper within the nasal tissue.

As further evidence of function, we tested for DC migration by two sets of studies. In the first, fluorescent OVA was tracked in the nasal tissues as well as draining LNs over time. A majority of the OVA+ nasal DCs (~99%) were CD103CD11b+CD64 that appeared within the cLN by 6h and progressively increased over 12–24h. The kinetics of response to Salmonella-mCherry were, however, much different. Within 5min of application, although a majority of the bacteria were in the nasal lumen, we could also detect Salmonellae intracellularly within the nasal CD11c+ cells. Furthermore, ~50% of the administered bacteriae (109 cfu) were cleared from the nose within half an hour of administration and could be detected in local draining LNs by 35min. The kinetics would suggest a DC-independent migration of Salmonellae into the draining LN. To further study this, we administered PTX, a known inhibitor of DC migration. This resulted in a significantly greater bacterial retention within the nose at 35min and a corresponding reduction in the Salmonella count within the draining LN. This difference between PBS and PTX-administered mice persisted over 12h. These data suggest a rapid transport of Gram-negative bacteria into the draining LN by the nasal DCs.

To lend clinical relevance to our mouse studies, we studied nasal DCs in human volunteers over a 12-month period. The goal of our human studies was to define the DC subsets in nasopharyngeal tissue and to determine whether a change in DC frequency is associated with chronic inflammation. Notably, previous studies have described human nasal DCs using a combination of HLA-DR, CD11c, and CD1c expression.48, 49 Here, by using a detailed, 12-color flow-cytometry panel, we found that in addition to BDCA-2+ plasmacytoid DCs, human nose biopsies encompass two other subsets of DCs: a major BDCA-1+ population and a smaller BDCA-3hi population. Interestingly, patients with CRS had a significantly lower frequency of BDCA-1+ DCs than normal individuals, while the BDCA-3hi DC reduction also trended toward statistical significance. Detailed functional or transcriptomic assessments were limited by the number of cells available to us in this study. However, in many of the human biopsy samples that we studied for DC subsets, microbial studies done as part of clinical care demonstrated a persistence of coagulase-negative staphylococci. This, combined with the observed reduction in BDCA-1+ and BDCA-3hi DCs in CRS, makes us hypothesize that a reduction in DCs, the key antigen-presenting cells within the nose, may predispose to impaired pathogen clearance and chronic inflammation. An alternative possibility is that nasal DCs may migrate into the draining LN during chronic inflammation, leading to falsely lowered numbers seen in the surgical specimens.22 As obtaining tissue from patients before the onset of CRS is impossible, we are unable to test this hypothesis. Finally, although we tried to control for the effect of treatment by a careful analysis of all concomitant medications, it is likely to be that treatment may have confounding effect on our results. It is has been shown that nasal plasmacytoid DC and mDC subsets are significantly reduced following steroid use22 and a number of patients in the CRS with nasal polyp group were treated with steroids before surgery. Regardless, further testing of the possibility that reduced nasal DC frequency may impair pathogen clearance from the nose and predispose to CRS may help identify the role of therapeutic vaccination strategies (targeting nasal DCs) to augment immune responses against specific pathogens in the treatment of patients with CRS.

To summarize, in this paper we provide the first detailed phenotypic and functional description of murine nasal DCs and describe a significant decrease in DC number to be associated with chronic naso-pharyngeal inflammation in humans. In doing so, we believe that the present study will direct the design of novel preventative and therapeutic vaccine strategies against human diseases.

Top

Methods

Human studies

Human nasal samples. Human nasal and nasopharyngeal tissues were obtained from patients undergoing otorhinological surgery for clinically indicated reasons at the Mount Sinai Hospital. Clinical details of the patients are listed in Supplementary Table S1. Informed consent was obtained from the patients. All samples were collected according to protocols approved by the Institutional Review Board at the Icahn School of Medicine at Mount Sinai.

Analyses of human nasal DCs. The human nasopharyngeal samples were processed immediately post surgery. The tissue was minced, treated with 0.25mgml−1 of collagenase (Sigma-Aldrich, St Louis, MO) at 37°C for 60min and cell isolation was performed. Cells were surface stained with CD45, CD20, CD3, CD335, CD66b, BDCA-1, BDCA-2, BDCA-3, CD14, and Live/Dead Fixable Blue Dead Cell Stain (Life Technologies, Grand Island, NY). Titrated antibodies were added to the cells in 50μl PBS 1% fetal calf serum for 30min at 4°C. Washed cells were fixed in 2% formaldehyde and stored at 4°C until analysis, which was performed using an LSR II flow cytometer (BD Biosciences, San Jose, CA). The whole sample was acquired and analysis was preformed using Flow Jo 9.1 software (Tree Star, Ashland, OR). To identify human DC subsets, dead cells were excluded and CD3+, CD20+, CD335+, CD66b+, and CD14+ cells, i.e., T, B, NK cells, neutrophils, and monocytes were sequentially gated out; three DC populations were identified within the CD45+ mononuclear cells by gating respectively on either BDCA-1+ cells, BDCA-2+ cells, or BDCA-3hi cells.

Fluorescent antibodies used. For human antibodies, we used monoclonal antibodies conjugated to different fluorochromes to anti-BDCA-1 (L161, Biolegend, San Diego, CA), anti-BDCA-2 (AC144, Miltenyi Biotec, San Diego, CA), anti-BDCA-3 (AD5-14H12, Miltenyi Biotec), anti-CD45 (HI30, BD Horizon, San Jose, CA). The following antibodies of anti-CD3 (OKT3), anti-CD20 (2H7), anti-CD66b (G10F5), and anti-CD335 (9E2) were obtained from Biolegend. The antibody to CD14 (TüK4) was obtained from Invitrogen (Grand Island, NY).

Mouse studies

Mice. Animal care and experimentation were consistent with guidelines of the US National Institutes of Health and were approved by the Institutional Animal Care and Use Committee of Mount Sinai School of Medicine. C57BL/6 mice (B6) and Balb/c mice were purchased from Taconic Farms (Hudson, NY) or bred at Mount Sinai School of Medicine. CD11c-EYFP reporter mice14 were purchased from Jackson Laboratory (Bar Harbor, ME). C57BL/6-Tg(TcraTcrb)425Cbn/J (OT-II) mice were kindly provided by Dr Shu-Hsia Chen (Mount Sinai School of Medicine). zDCDTR bone marrow chimeric mice were generously provided by Dr M.C. Nussenzweig39 (Rockefeller University). MMDTR41 or C57BL/6 bone marrow chimeric mice were obtained from the laboratory of Dr Daniel Mucida (Rockefeller University). All animals were housed under specific pathogen-free conditions and killed at the indicated time points.

Bone marrow chimeras were created by transferring bone marrow from zDCDTR, MMDTR, and C57BL/6 mice into lethally irradiated WT C57BL/6 mice, to avoid lethality associated with DT treatments of zDCDTR or MMDTR. In brief, 7-week-old male C57BL/6 hosts underwent two treatments of 500rads in an animal γ-irradiator 3-h apart, and 2.5 × 106 bone marrow cells from 8- to 12-week-old zDCDTR, MMDTR, and C57BL/6 (WT) donors, respectively, were transferred intravenously. The resulting mice (referred to herein as zDCDTR, MMDTR, and WT) were housed under specific pathogen-free conditions. The chimeric mice were fully reconstituted and ready for experimental use after 8 weeks.

Isolation of NALT. Mice were euthanized by CO2 inhalation and an incision was made at the angle of the mouth on both sides to open the mouth and expose the palate, using established protocols.23 Using curved fine forceps, the NALT bearing hard palate was slowly peeled and immediately embedded in to OCT (optimal cutting temperature; Sakura Finetek, Torrance, CA), and stored at −80°C. Nasal sections were cut at 10-μm thickness with a Leica cryostat (Leica Microsystems, Buffalo Grove, IL) and stored at −80°C.

Histology and immunofluorescence analysis. Nasal cryosections were stained with hematoxylin and eosin and fluorescence-activated cell-sorted CD11c+MHCII+CD11b+F4/80CD64 and CD11c+MHCII+CD11b+F4/80+CD64+ nasal cells were centrifuged by cytospin onto glass sides, stained in Wright–Geimsa stain solution. Images were captured by brightfield microscope (Zeiss Axioplan2IE, Carl Zeiss, Thornwood, NY) and AxioVision software (Carl Zeiss). Ten-micrometer nasal sections were briefly air dried, fixed in cold acetone for 15min, rehydrated in PBS for 10min, blocked with 5% bovine serum albumin/0.01% Tween/PBS for 30min at room temperature. Sections were stained in a humidified chamber for 1 or 2h at room temperature or overnight at 4°C with primary antibodies (Supplementary Table S2), washed with 0.01% Tween/PBS for 5min three times, and stained for 1h at room temperature with secondary antibodies. If necessary, fluorochrome-labeled tertiary antibody was applied. T cells were immunostained with rabbit anti-mouse CD3 followed by anti-rabbit IgG Alexa Fluor 488, B cells with rat anti-mouse B220 followed by anti-rat IgG Alexa Fluor 647, and DCs with hamster anti-mouse CD11c followed by both biotinylated anti-hamster IgG and streptavidin Alexa Flour 555. Epithelial cells were immunostained with rabbit anti-mouse cytokeratin 18 followed by anti-rabbit IgG Alexa Fluor 488. Endothelial cells, lining lymphatic vessels were stained with rabbit anti-mouse LYVE-1 followed by anti-rabbit IgG Alexa Fluor 488. The longitudinal sections of NALT were stained with rat anti-mouse PECAM-1 followed by anti-rat IgG Alexa Fluor 488. Sections were stained with goat anti-mouse langerin (E17) followed by donkey anti-goat Alexa 488. Sections were mounted in ProLong Gold Antifade Mounting Medium with 4′, 6-diamidinio-2-phenylindole (Life Technologies). Fluorescent images were examined in laser scanning confocal microscope (SP5 DM, Leica Microsystems) and LAS AF software (Leica Microsystems) at the Microscopy Shared Resource Facility of Mount Sinai School of Medicine and epi-fluorescence microscope (Eclipse Ni-U, Nikon Instruments, Melville, NY) with NIS Elements BR software (Nikon Instruments) at the Immunology Institute of Mount Sinai School of Medicine.

Two-photon microscopy. After euthanizing mice, the NALT and LNs were surgically isolated and transferred to a 25 × 25 × 1mm imaging chamber (Grace Bio-Labs, Bend, OR) containing RPMI. Two-photon imaging was performed using an Olympus Fluoview FV1200MPE microscope with a 40 × 0.8 numerical aperture water-emersion objective lens. For multiphoton excitation and second harmonic generation, a MaiTai Ti:sapphire laser (Newport/Spectra-Physics, Santa Clara, CA) was tuned to 790 and 890nm for optimized excitation of mCherry expressing Salmonella and CD11c-eYFP+ cells, respectively. Emission filters were 420–460 for second harmonic generation (SHG), 495–540nm for enhanced yellow fluorescent protein (eYFP), and 575–630nm for mCherry. Six to eight 80-μm z-stacks were acquired for each NALT and two to four 200-μm z-stacks were acquired for each LN, with 2-μm z-spacing between each optical section (512 × 512 pixels). Three-dimensional reconstruction and image analysis was performed using watershed segmentation algorithms in Imaris (Bitplane, South Windsor, CT), to quantify numbers of mCherry+ and CD11c-eYFP+ cells.

Nasal, splenic, and LN single-cell preparation. After euthanizing mice, the entire nose was dissected out using previously reported methods.23 The organ was mechanically disrupted by using scissors and transferred to conical tubes. The tissue pieces were re suspended in 10ml of RPMI containing 0.3mgml−1 of collagenase (Sigma-Aldrich) and shaken at 200r.p.m. for 60min at 37°C. The tissue suspension was collected and passed through a 70-μm cell strainer and the cells were pelleted by centrifugation at 1,600r.p.m. for 5min. The nasal cells were then resuspended in RPMI. For analyses of NALT and non-NALT DCs separately, the NALT was dissected out as mentioned in the preceding sections. The remaining nasal tissue and NALT tissue were then collagenase digested as mentioned above.

For spleen cell preparation, ACK lysis of red blood cells was followed by washing twice and filtering undigested fibrous material through a 70-μm cell strainer.

For LN single-cell preparation, LNs were isolated and mechanically disrupted. LN single-cell suspensions were prepared by incubation with 400Uml−1 collagenase D at 37°C for 30min. Cells were filtered through a 70-μm cell strainer.

Nasal DC isolation. Single-cell suspensions were isolated from the nose as described above. Using magnetic beads (Miltenyi Biotec), CD11c+ cells were isolated as previously described.50 Briefly, the cells were washed in 1ml of MACS buffer (Miltenyi Biotec) before 30min incubation on ice with CD11c+ beads. The cellular suspensions were washed twice in MACS buffer and passed through a magnetic column, CD11c+ cells were isolated by positive selection, washed, and resuspended in PBS. The subsets of CD11c+ enriched cells were then sorted on FACSAria (BD Biosciences) as CD11c+MHCII+CD11b+F4/80CD64 and CD11c+MHCII+CD11b+F4/80+CD64+ cells.

Fluorescent antibodies used. We used the following murine antibodies, detailed in Supplementary Table S3, to anti-CD3 (17A2), anti-CD4 (RM4-5), anti-CD8α (53-6.7), anti-CD103 (M290), anti-Ly6G (1A8), and anti-CD64 (X54-5/7.1) from BD Biosciences; anti-CD11b (M1/70), anti-CD11c (N418), anti-CD45 (30-F11), anti-CD19 (6D5), anti-F4/80 (BM8), anti-Ly6C (HK1.4), anti-CD24 (M1/69), anti-TLR3 (11F8), anti-TCR Vα2 (B20.1), anti-CD14 (Sa14-2), and anti-MMR/CD206 (C068C2) from Biolegend; anti-CD3e (145-2C11), anti-EpCAM (G8.8), anti-CD24 (M1/69), CD115 (AFS98), anti-MHCII (M5/114.15.2), anti-CD207 (L31), anti-CD317 (129c), anti-CD45R (RA3-6B2), and anti-NK1.1 (PK136) from eBioscience (San Diego, CA). AQUA (L34957) was from Life Technologies and CFSE was from Sigma-Aldrich.

Flow cytometric analyses and gating. Cells were isolated as described above. Before staining, cells were washed and resuspended in staining buffer containing 1 × PBS, 2% bovine serum albumin, 10mM EDTA, and 0.01% NaN3. To block nonspecific staining, the 2.4G2 anti-CD16/32 antibody was added. Antibodies for cell surface markers were added and cells were incubated for 25min at 4°C. Following the staining, the cells were washed twice and analyzed immediately or fixed in PBS containing 1% paraformaldehyde and 0.01% NaN3, and analyzed later on an LSR Fortessa (BD Biosciences), using multiparameter flow cytometry.

For intracellular staining, post-surface staining cells were resuspended in Fixation/Permeablization solution (BD Cytofix/Cytoperm kit, BD Biosciences), and intracellular cytokine staining was performed according to the manufacturer’s protocol. Flow cytometric data was analyzed with FlowJo software (Tree Star). Myeloid nasal DC cells were identified by forward and side-scatter characteristics, and dead cells were excluded using Live-dead fixable Aqua (Life Technologies) followed by sequential gating on CD45+, MHCII+, CD11c+, CD11b+ or CD103+, or CD11b+CD1103+ double-positive populations.

B16-FLT3L-induced cell expansion in vivo. B16 melanoma cells (B16) and B16 melanoma cells expressing FLT3L (B16-FLT3L) were a provided by Dr Andrew Sikora. Cells were cultured in Dulbecco’s modified Eagle’s medium containing 10% fetal calf serum and antibiotics. Mice were injected intradermally with 5–7 × 106 B16 or B16-FLT3L cells. The nasal DC subsets were analyzed 10–14 days later.

Nasal sample processing by mass cytometry. The entire nose from B16 or B16-FLT3L cells implanted mice were processed as described above and stained for CyTOF analysis. All CyTOF-associated reagents were purchased from Fluidigm (Sunnyvale, CA, formerly DVS Sciences). In brief, 5 million cells were stained with a panel of 20 metal-conjugated antibodies (Supplementary Table S4) for 20min on ice. All antibodies were either purchased pre-conjugated or conjugated and validated in-house using MaxPar X8 conjugation kits, according to the manufacturer’s protocols. The samples were then incubated for 5min in cisplatin to identify dead cells, washed, and resuspended in fix/perm buffer containing Ir nucleic acid intercalator to label all nucleated cells. Immediately before analysis, the cells were washed in PBS and diH20 and resuspended in diH20 containing a 1:10 dilution of EQ 4 Element Calibration beads. After instrument tuning, the samples were acquired on a CyTOF2 Mass Cytometer (Fluidigm) in sequential 10-min acquisitions at a rate of <400 events per second. Following data acquisition, the FCS files were concatenated and normalized using a bead-based normalization algorithm within the CyTOF acquisition software and uploaded to Cytobank for analysis. The FCS files were manually pre-gated on Ir-193 DNA+CD45+ singlets excluding beads, doublets, DNA-negative cell debris, and Pt-195 cisplatin+ dead cells. The gated population was clustered using spanning-tree progression analysis of density-normalized events.24 Cell populations on the spanning-tree progression analysis of density-normalized event trees were manually annotated and based on expression of key markers, while also preserving visualization of unidentified populations within the data set.

In vivo DT treatment. DT purchased from Sigma-Aldrich was prepared in a sterile solution of PBS at a concentration of 1mgml−1. DC depletion was effected in zDCDTR and MMDTR chimeras by a single intraperitoneal injection of 500ng DT. In addition, WT bone marrow chimeras also received 500ng DT per mouse. To analyze cell depletion, mice were euthanized 24h after DT injection.

In vitro DC: T-cell co-cultures, antigen-presentation assays, and MLR. The culture medium used for DC and T-cell cultures was RPMI (Gibco, Grand Island, NY) supplemented with 10% heat-inactivated fetal calf serum, 2mM L-glutamine, 100Uml−1 penicillin, 100μgml−1 streptomycin, and 5mM β-mercaptoethanol (all from Sigma-Aldrich). For antigen-presentation assays, OT-II T cells (for the MLR, T cells from BALB/c spleen) were isolated from spleens of C57BL/6 mice by excluding B220+, F4/80+, CD49b+, and I-Ab+ cells with anti-rat IgG Dynabeads (Life Technologies) and labeled with 5μM CFSE (Life Technologies) for 10min at 37°C. CFSE-labeled T cells were added to round-bottom microtest wells at 5 × 104 per well and mixed with isolated DCs at a DC:T cell ratio of 1:10, 1:30, and 1:100 in the presence of OVA (25gml−1 final) for antigen-presentation assays. After 5 days, the proliferating T cells were evaluated by fluorescence-activated cell sorting.

LPS administration and OVA-Alexa Fluor 488. LPS of Escherichia coli was purchased from Sigma-Aldrich and prepared in a sterile solution of PBS at a concentration of 1mgml−1. OVA-Alexa Fluor 488 (Life Technologies) was reconstituted at 15μgμl−1 with a sterile solution of PBS. One hundred and fifty milligrams of OVA-Alexa Fluor 488 was co-administered with 1μg of LPS in a final volume of 11μl intranasally. Mice were killed at 1, 6, 12, and 24h later and nasal tissues, cLN, and mediastinal LN were examined for OVA+ cells.

Infection. For mCherry expressing Salmonella infection, mice were infected intranasally with 109 cfu per mouse. Where specified, 1μg PTX (List Biological Laboratories, Campbell, CA) per mouse was supplemented with Salmonella. Dr Daniel Mucida kindly provided us with mCherry expressing Salmonella. Appearance of infected mice was monitored. Cells from the nose and cervical LNs were analyzed at 5min, 35min, and 12h post infection.

Statistical analysis. Data were entered into a Microsoft Excel database and Prism 6 program (GraphPad Software, La Jolla, CA) was used for quantitative data and statistical analyses. Statistical significance was evaluated using a one-way analysis of variance test followed by a post-hoc Bonferroni test with a 95% confidence interval. Results are expressed as means±s.d.

*P<0.05, **P<0.01, or ***P<0.001 was considered to be statistically significant.

Top

References

  1. Kurono, Y. et al. Nasal immunization induces Haemophilus influenzae-specific Th1 and Th2 responses with mucosal IgA and systemic IgG antibodies for protective immunity. J. Infect. Dis. 180, 122–132 (1999). | Article | PubMed | ISI | CAS |
  2. Yanagita, M. et al. Nasopharyngeal-associated lymphoreticular tissue (NALT) immunity: fimbriae-specific Th1 and Th2 cell-regulated IgA responses for the inhibition of bacterial attachment to epithelial cells and subsequent inflammatory cytokine production. J. Immunol. 162, 3559–3565 (1999). | PubMed | ISI | CAS |
  3. Sandoval, F. et al. Mucosal imprinting of vaccine-induced CD8(+) T cells is crucial to inhibit the growth of mucosal tumors. Sci. Transl. Med. 5, 172ra20 (2013). | Article | PubMed | CAS |
  4. Langermann, S., Palaszynski, S., Sadziene, A., Stover, C.K. & Koenig, S. Systemic and mucosal immunity induced by BCG vector expressing outer-surface protein A of Borrelia burgdorferi. Nature 372, 552–555 (1994). | Article | PubMed | ISI |
  5. Velge-Roussel, F., Marcelo, P., Lepage, A.C., Buzoni-Gatel, D. & Bout, D.T. Intranasal immunization with Toxoplasma gondii SAG1 induces protective cells into both NALT and GALT compartments. Infect. Immun. 68, 969–972 (2000). | Article | PubMed |
  6. Ruane, D. et al. Lung dendritic cells induce migration of protective T cells to the gastrointestinal tract. J. Exp. Med. 210, 1871–1888 (2013). | Article | PubMed | CAS |
  7. Gautier, E.L. et al. Gene-expression profiles and transcriptional regulatory pathways that underlie the identity and diversity of mouse tissue macrophages. Nat. Immunol. 13, 1118–1128 (2012). | Article | PubMed | ISI | CAS |
  8. Imaoka, K. et al. Nasal immunization of nonhuman primates with simian immunodeficiency virus p55gag and cholera toxin adjuvant induces Th1/Th2 help for virus-specific immune responses in reproductive tissues. J. Immunol. 161, 5952–5958 (1998). | PubMed | ISI | CAS |
  9. Hiroi, T. et al. HIV mucosal vaccine: nasal immunization with rBCG-V3J1 induces a long term V3J1 peptide-specific neutralizing immunity in Th1- and Th2-deficient conditions. J. Immunol. 167, 5862–5867 (2001). | Article | PubMed | ISI | CAS |
  10. Kuper, C.F., Hameleers, D.M., Bruijntjes, J.P., van der Ven, I., Biewenga, J. & Sminia, T. Lymphoid and non-lymphoid cells in nasal-associated lymphoid tissue (NALT) in the rat. An immuno- and enzyme-histochemical study. Cell Tissue Res. 259, 371–377 (1990). | Article | PubMed | ISI | CAS |
  11. Kuper, C.F. et al. The role of nasopharyngeal lymphoid tissue. Immunol. Today 13, 219–224 (1992). | Article | PubMed | ISI | CAS |
  12. Yao, Q., Vuong, V., Li, M. & Compans, R.W. Intranasal immunization with SIV virus-like particles (VLPs) elicits systemic and mucosal immunity. Vaccine 20, 2537–2545 (2002). | Article | PubMed | ISI |
  13. Fukuyama, S. et al. Initiation of NALT organogenesis is independent of the IL-7R, LTbetaR, and NIK signaling pathways but requires the Id2 gene and CD3(-)CD4(+)CD45(+) cells. Immunity 17, 31–40 (2002). | Article | PubMed | ISI | CAS |
  14. Lindquist, R.L. et al. Visualizing dendritic cell networks in vivo. Nat. Immunol. 5, 1243–1250 (2004). | Article | PubMed | ISI | CAS |
  15. Wu, Y., Wang, X., Csencsits, K.L., Haddad, A., Walters, N. & Pascual, D.W. M cell-targeted DNA vaccination. Proc. Natl Acad. Sci. USA. 98, 9318–9323 (2001). | Article | PubMed | CAS |
  16. Jeong, K.I., Suzuki, H., Nakayama, H. & Doi, K. Ultrastructural study on the follicle-associated epithelium of nasal-associated lymphoid tissue in specific pathogen-free (SPF) and conventional environment-adapted (SPF-CV) rats. J. Anat. 196 (Pt 3), 443–451 (2000). | Article | PubMed | ISI |
  17. Spit, B.J., Hendriksen, E.G., Bruijntjes, J.P. & Kuper, C.F. Nasal lymphoid tissue in the rat. Cell Tissue Res. 255, 193–198 (1989). | Article | PubMed | ISI |
  18. Nagatake, T. et al. Id2-, RORgammat-, and LTbetaR-independent initiation of lymphoid organogenesis in ocular immunity. J. Exp. Med. 206, 2351–2364 (2009). | Article | PubMed | ISI | CAS |
  19. Sosa, G.A. & Roux, M.E. Development of T lymphocytes in the nasal-associated lymphoid tissue (NALT) from growing Wistar rats. Clin. Dev. Immunol. 11, 29–34 (2004). | Article | PubMed |
  20. Costalonga, M., Cleary, P.P., Fischer, L.A. & Zhao, Z. Intranasal bacteria induce Th1 but not Treg or Th2. Mucosal Immunol. 2, 85–95 (2009). | Article | PubMed | ISI | CAS |
  21. Kiyono, H. & Fukuyama, S. NALT- versus Peyer's-patch-mediated mucosal immunity. Nat.Rev. Immunol. 4, 699–710 (2004). | Article |
  22. Hartmann, E. et al. Analysis of plasmacytoid and myeloid dendritic cells in nasal epithelium. Clin. Vaccine Immunol. 13, 1278–1286 (2006). | Article | PubMed | ISI |
  23. Heritage, P.L., Underdown, B.J., Arsenault, A.L., Snider, D.P. & McDermott, M.R. Comparison of murine nasal-associated lymphoid tissue and Peyer’s patches. Am. J. Respir. Crit. Care Med. 156 (4 Pt 1), 1256–1262 (1997). | Article | PubMed | CAS |
  24. Qiu, P. et al. Extracting a cellular hierarchy from high-dimensional cytometry data with SPADE. Nat. Biotechnol. 29, 886–891 (2011). | Article | PubMed | ISI | CAS |
  25. Niess, J.H. et al. CX3CR1-mediated dendritic cell access to the intestinal lumen and bacterial clearance. Science 307, 254–258 (2005). | Article | PubMed | ISI | CAS |
  26. Jakubzick, C. et al. Minimal differentiation of classical monocytes as they survey steady-state tissues and transport antigen to lymph nodes. Immunity 39, 599–610 (2013). | Article | PubMed | ISI | CAS |
  27. Banchereau, J. & Steinman, R.M. Dendritic cells and the control of immunity. Nature 392, 245–252 (1998). | Article | PubMed | ISI | CAS |
  28. Liu, Y.J., Kanzler, H., Soumelis, V. & Gilliet, M. Dendritic cell lineage, plasticity and cross-regulation. Nat. Immunol. 2, 585–589 (2001). | Article | PubMed | ISI | CAS |
  29. Kataoka, K. et al. The nasal dendritic cell-targeting Flt3 ligand as a safe adjuvant elicits effective protection against fatal pneumococcal pneumonia. Infect. Immun. 79, 2819–2828 (2011). | Article | PubMed |
  30. Nacer, A. et al. Imaging murine NALT following intranasal immunization with flagellin-modified circumsporozoite protein malaria vaccines. Mucosal Immunol. 7, 304–314 (2014). | Article | PubMed | ISI |
  31. Tamoutounour, S. et al. Origins and functional specialization of macrophages and of conventional and monocyte-derived dendritic cells in mouse skin. Immunity 39, 925–938 (2013). | Article | PubMed | ISI | CAS |
  32. Schlitzer, A. et al. IRF4 transcription factor-dependent CD11b+ dendritic cells in human and mouse control mucosal IL-17 cytokine responses. Immunity 38, 970–983 (2013). | Article | PubMed | ISI | CAS |
  33. Kataoka, K., McGhee, J.R., Kobayashi, R., Fujihashi, K., Shizukuishi, S. & Fujihashi, K. Nasal Flt3 ligand cDNA elicits CD11c+CD8+ dendritic cells for enhanced mucosal immunity. J. Immunol. 172, 3612–3619 (2004). | Article | PubMed | ISI | CAS |
  34. Jakubzick, C., Helft, J., Kaplan, T.J. & Randolph, G.J. Optimization of methods to study pulmonary dendritic cell migration reveals distinct capacities of DC subsets to acquire soluble versus particulate antigen. J. Immunol. Methods 337, 121–131 (2008). | Article | PubMed | ISI | CAS |
  35. Maraskovsky, E. et al. Dramatic increase in the numbers of functionally mature dendritic cells in Flt3 ligand-treated mice: multiple dendritic cell subpopulations identified. J. Exp. Med. 184, 1953–1962 (1996). | Article | PubMed | ISI | CAS |
  36. Fukuiwa, T. et al. A combination of Flt3 ligand cDNA and CpG ODN as nasal adjuvant elicits NALT dendritic cells for prolonged mucosal immunity. Vaccine 26, 4849–4859 (2008). | Article | PubMed | ISI |
  37. Varmus, H., Klausner, R., Zerhouni, E., Acharya, T., Daar, A.S. & Singer, P.A. Public health. grand challenges in global health. Science 302, 398–399 (2003). | Article | PubMed | ISI | CAS |
  38. Bendall, S.C. et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science 332, 687–696 (2011). | Article | PubMed | ISI | CAS |
  39. Meredith, M.M. et al. Expression of the zinc finger transcription factor zDC (Zbtb46, Btbd4) defines the classical dendritic cell lineage. J. Exp. Med. 209, 1153–1165 (2012). | Article | PubMed | ISI | CAS |
  40. Satpathy, A.T. et al. Zbtb46 expression distinguishes classical dendritic cells and their committed progenitors from other immune lineages. J. Exp. Med. 209, 1135–1152 (2012). | Article | PubMed | ISI | CAS |
  41. Schreiber, H.A. et al. Intestinal monocytes and macrophages are required for T cell polarization in response to Citrobacter rodentium. J. Exp. Med. 210, 2025–2039 (2013). | Article | PubMed | ISI | CAS |
  42. Steinman, R.M. & Witmer, M.D. Lymphoid dendritic cells are potent stimulators of the primary mixed leukocyte reaction in mice. Proc. Natl Acad. Sci. USA 75, 5132–5136 (1978). | Article | PubMed | CAS |
  43. Steinman, R.M. & Cohn, Z.A. Identification of a novel cell type in peripheral lymphoid organs of mice. I. Morphology, quantitation, tissue distribution. J. Exp. Med. 137, 1142–1162 (1973). | Article | PubMed | ISI | CAS |
  44. Jabbal-Gill, I., Watts, P. & Smith, A. Chitosan-based delivery systems for mucosal vaccines. Expert Opin. Drug Deliv. 9, 1051–1067 (2012). | Article | PubMed | ISI |
  45. Nochi, T. et al. Nanogel antigenic protein-delivery system for adjuvant-free intranasal vaccines. Nat. Mater. 9, 572–578 (2010). | Article | PubMed | ISI | CAS |
  46. Bogunovic, M. et al. Origin of the lamina propria dendritic cell network. Immunity 31, 513–525 (2009). | Article | PubMed | ISI | CAS |
  47. Sekine, S. et al. A novel adenovirus expressing Flt3 ligand enhances mucosal immunity by inducing mature nasopharyngeal-associated lymphoreticular tissue dendritic cell migration. J. Immunol. 180, 8126–8134 (2008). | Article | PubMed | ISI | CAS |
  48. Jahnsen, F.L., Gran, E., Haye, R. & Brandtzaeg, P. Human nasal mucosa contains antigen-presenting cells of strikingly different functional phenotypes. Am. J. Respir. Cell. Mol. Biol. 30, 31–37 (2004). | Article | PubMed | ISI | CAS |
  49. Takano, K. et al. HLA-DR- and CD11c-positive dendritic cells penetrate beyond well-developed epithelial tight junctions in human nasal mucosa of allergic rhinitis. J. Histochem. Cytochem. 53, 611–619 (2005). | Article | PubMed |
  50. Ing, R., Segura, M., Thawani, N., Tam, M. & Stevenson, M.M. Interaction of mouse dendritic cells and malaria-infected erythrocytes: uptake, maturation, and antigen presentation. J. Immunol. 176, 441–450 (2006). | Article | PubMed | ISI | CAS |
Top

Acknowledgments

This work was inspired by Dr Ralph Steinman. We thank Dr Maggi Pack for her help with immunofluorescent staining, Dr Michelle Gaylord for her help with tissue processing, and Dr Chae Gyu Park for valuable discussion. This work was supported by grants from the American Gastroenterology Association Elsevier Award (S.M.), The Rockefeller University Clinical and Translation science award pilot project from NIH/NCRR (5UL1RR024143-05) National Institutes of Health (S.M. and G.B., grant 8 UL 1 TR000043); B.C. and K.L. grants DP1DA028866 and F31DA036425, respectively, The Leona M. and Harry B. Helmsley Charitable Trust (S.M and G.B), as well as the Crohn’s & Colitis Foundation of America (S.M.)

SUPPLEMENTARY MATERIAL is linked to the online version of the paper