Mucosal Immunology

FIGURE 1

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Cholera toxin, E. coli heat-labile toxin, and non-toxic derivatives induce dendritic cell migration into the follicle-associated epithelium of Peyer's patches

N G Anosova, S Chabot, V Shreedhar, J A Borawski, B L Dickinson and M R Neutra

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Figure 1.

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CT and CTB injected into ligated ileal loops induce migration of DCs into the FAE. (a) Control PP tissue section stained with DAPI (blue) and anti-laminin (red) to visualize the boundary between the SED region and the FAE. (bd) Sections of PP domes stained for CD11c (green) and laminin (red), taken from ligated ileal loops injected with (b) PBS, (c) CT, or (d) CTB 90 min earlier. Increased numbers of DCs (arrowheads) are present in the FAE following injection of either CT or CTB. The images shown are representative of sections from four BALB/c mice per group. CT, cholera toxin; DAPI, 4,6-diamidino-2-phenylindole; DCs, dendritic cells; FAE, follicle-associated epithelium; PBS, phosphate-buffered saline; PPs, Peyer's patches; SED, subepithelial dome. Bar=10 mum.

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