FIGURE 3

Induction of CCR9, 47, and E-selectin ligand expression in vitro is antigen dose dependent. (a–c) Purified CD11c⁺ MLN or splenic DCs (10⁵) were pulsed with pOVA and incubated with 2 10⁵ CFSE-labeled OT-I cells. After 4 days of culture, OT-I cells were analyzed for expression of (a–c) CCR9 and (b and c) 47 by flow cytometry. (b) Bars represent means.e.m. of the percentage positive OT-I cells (n=6). **P<0.01. (c) OT-I cells were analyzed for CCR9 and 47 expression as a function of division. Means.e.m. from six experiments. *P<0.05, **P<0.01; asterisks are placed between compared groups. (d) Induction of CCR9 and E-selectin ligand expression by CD103⁺ and CD103^- MLN DCs is antigen dose dependent. Purified CD103⁺ and CD103^- CD11c⁺ MLN DCs (10⁵) were pulsed with pOVA and incubated with 2 10⁵ CFSE-labeled OT-I cells. OT-I cells were analyzed for CCR9 and E-selectin ligand expression after 4 and 6 days of culture, respectively. Bars represent means.e.m. of the percentage positive OT-I cells (n=3–7). *P<0.05. CCR, CC chemokine receptor; DC, dendritic cell; MLN, mesenteric lymph node; pOVA, ovalbumin peptide; SPL, splenic.