FIGURE 4
FROM:
Immunization with adenovirus at the large intestinal mucosa as an effective vaccination strategy against sexually transmitted viral infection
Q Zhu, C W Thomson, K L Rosenthal, M R McDermott, S M Collins and J Gauldie
BACK TO ARTICLEFigure 4.
ICR immunization induces effective local mucosal immunity at both colorectal and vaginal mucosa. (a) Mice were immunized with AdOVA by ICR (2 
109 PFU), IN (5 108 PFU), or SC (5 108 PFU). Both OVA-specific IFN-
-secreting LPLs (left panel) and local IgA production in colorectal homogenates (right panel) were compared after 2 weeks. (b) Animals were challenged rectally (left panel) with 5 107 PFU or vaginally (right panel) with 2 107 PFU of VV-OVA. Six days later, local tissue and paired ovaries were examined for infectious viral particles by plaque assays. Results represent one of two independent experiments and are expressed as mean
s.e.m. from three mice in each group. *, **, and *** indicate P<0.05, 0.01, and 0.001, respectively. AdOVA, Ad expressing ovalbumin; ICR, intracolorectal; PFU, plaque-forming unit; IN, intranasal; SC, subcutaneous; IFN-, interferon-; LPL, lamina propria lymphocyte; VV-OVA, vaccinia virus expressing OVA.