FIGURE 2

Oral immunization of L-Sel^-/- mice with CT results in increased IgA AFC responses in CLN 16 days post-primary immunization. L-Sel^-/- and L-Sel^+/+ mice were orally immunized, as previously described, and on day 16, total lymphocytes from (a, b) NP and small iLP, (c, d) NALT and PP, and (e, f) SMLN, PRLN, and CLN were collected and assayed for anti-CT-B and total (a, c, e) IgA and (b, d, f) IgG AFC responses. CT-B-specific and total IgA AFC responses were slightly enhanced in L-Sel^-/- CLN as well as total IgG AFCs. The number of IgA and IgG CT-B-specific AFCs in the other L-Sel^-/- HNLN, NALT, PP, NP, and iLP were not significantly different from L-Sel^+/+ mice. Results depict the mean of three experiments s.e.m. per tissue, and statistical differences between L-Sel^-/- and L-Sel^+/+ mice were determined: *P<0.001; **P0.003; ***P=0.031. AFC, antibody-forming cell; CLN, cervical lymph node; CT, cholera toxin; HNLN, head and neck lymph node; Ig, immunoglobulin; iLP, intestinal lamina propria; L-Sel, L-selectin; NALT, nasal-associated lymphoid tissue; NP, nasal passage; PP, Peyer's patch; PRLN, parotid gland lymph node; SMLN, submaxillary gland lymph node.