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Immunophenotyping of acute leukemia and lymphoproliferative disorders: a consensus proposal of the European LeukemiaNet Work Package 10

Leukemia volume 25, pages 567–574 (2011)Cite this article

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Abstract

The European LeukemiaNet (ELN), workpackage 10 (WP10) was designed to deal with diagnosis matters using morphology and immunophenotyping. This group aimed at establishing a consensus on the required reagents for proper immunophenotyping of acute leukemia and lymphoproliferative disorders. Animated discussions within WP10, together with the application of the Delphi method of proposals circulation, quickly led to post-consensual immunophenotyping panels for disorders on the ELN website. In this report, we established a comprehensive description of these panels, both mandatory and complementary, for both types of clinical conditions. The reason for using each marker, sustained by relevant literature information, is provided in detail. With the constant development of immunophenotyping techniques in flow cytometry and related software, this work aims at providing useful guidelines to perform the most pertinent exploration at diagnosis and for follow-up, with the best cost benefit in diseases, the treatment of which has a strong impact on health systems.

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Acknowledgements

We are grateful to all WP10 participants for their input in fruitful discussions and consensual work. This work was supported by the European LeukemiaNet, Network of Excellence, 6th PCRDT by the European Community.

Author information

Authors and Affiliations

  1. Immunology Laboratory, CHU de Brabois & Nancy Université, Vandoeuvre les Nancy, France

    M C Béné & G C Faure

  2. Onkologikum Labor, Frankfurt, Germany

    T Nebe

  3. Institut für Labordiagnostik, Allgemeines Krankenhaus, Linz, Austria

    P Bettelheim

  4. Pediatric Department, Pediatric Hematooncologic Laboratory, Padova University, Padova, Italy

    B Buldini

  5. Department of Hematology, Carol Davila University of Medicine, Bucharest, Romania

    H Bumbea & A M Vladareanu

  6. MLL Munich Leukemia Labor, München, Germany

    W Kern

  7. Haematology Laboratory, Centre Hospitalo-Universitaire, Haut Lévêque Bordeaux, Pessac, France

    F Lacombe

  8. Department of Hematology and Blood Transfusion, Hospital Jihlava, Jihlava, Czech Republic

    P Lemez

  9. Institute of Hematology and Blood Transfusion, Prague, Czech Republic

    I Marinov

  10. Haemato-Oncology, Institute of Cancer Research, London, UK

    E Matutes

  11. Haematology Laboratory, CHU Dijon, Dijon, France,

    M Maynadié

  12. Flow Cytometry Laboratory, Medizinische Klinik und Poliklinik I, Dresden, Germany

    U Oelschlagel

  13. Centro de Investigación del Cáncer, Lab Citometría, Salamanca, Spain

    A Orfao

  14. HELIOS Klinikum Berlin-Buch, Berlin, Germany

    R Schabath

  15. University Clinic for Hematology and Central Hematology Laboratory, Inselspital Bern and University of Bern, Bern, Switzerland

    M Solenthaler

  16. Labor für Hämatologische Spezialdiagnostik Interne I mit Hämatologie und Onkologie, Krankenhaus der Barmherzigen Schwestern, Linz, Austria

    G Tschurtschenthaler

  17. Haematology Laboratory, Catholic University, Rome, Italy

    G Zini

  18. Department of Pathology, Karolinska University Hospital and Institute, Stockholm, Sweden

    A Porwit

Authors
  1. M C Béné
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  2. T Nebe
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  3. P Bettelheim
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  4. B Buldini
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  18. G Zini
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  19. G C Faure
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  20. A Porwit
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Corresponding author

Correspondence to M C Béné.

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The authors declare no conflict of interest.

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Supplementary Information accompanies the paper on the Leukemia website

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Béné, M., Nebe, T., Bettelheim, P. et al. Immunophenotyping of acute leukemia and lymphoproliferative disorders: a consensus proposal of the European LeukemiaNet Work Package 10. Leukemia 25, 567–574 (2011). https://doi.org/10.1038/leu.2010.312

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  • DOI: https://doi.org/10.1038/leu.2010.312

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