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Absolute quantitative detection of ABL tyrosine kinase domain point mutations in chronic myeloid leukemia using a novel nanofluidic platform and mutation-specific PCR

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References

  1. Nicolini FE, Corm S, Le QH, Sorel N, Hayette S, Bories D et al. Mutation status and clinical outcome of 89 imatinib mesylate-resistant chronic myelogenous leukemia patients: a retrospective analysis from the French intergroup of CML (Fi(phi)-LMC GROUP). Leukemia 2006; 20: 1061–1066.

    Article  CAS  Google Scholar 

  2. Branford S, Rudzki Z, Walsh S, Parkinson I, Grigg A, Szer J et al. Detection of BCR-ABL mutations in patients with CML treated with imatinib is virtually always accompanied by clinical resistance, and mutations in the ATP phosphate-binding loop (P-loop) are associated with a poor prognosis. Blood 2003; 102: 276–283.

    Article  CAS  Google Scholar 

  3. Soverini S, Martinelli G, Rosti G, Bassi S, Amabile M, Poerio A et al. ABL mutations in late chronic phase chronic myeloid leukemia patients with up-front cytogenetic resistance to imatinib are associated with a greater likelihood of progression to blast crisis and shorter survival: a study by the GIMEMA Working Party on Chronic Myeloid Leukemia. J Clin Oncol 2005; 23: 4100–4109.

    Article  CAS  Google Scholar 

  4. Ottesen EA, Hong JW, Quake SR, Leadbetter JR . Microfluidic digital PCR enables multigene analysis of individual environmental bacteria. Science 2006; 314: 1464–1467.

    Article  CAS  Google Scholar 

  5. Baker SG, Kramer BS, McIntosh M, Patterson BH, Shyr Y, Skates S . Evaluating markers for the early detection of cancer: overview of study designs and methods. Clin Trials 2006; 3: 43–56.

    Article  Google Scholar 

  6. Willis SG, Lange T, Demehri S, Otto S, Crossman L, Niederwieser D et al. High-sensitivity detection of BCR-ABL kinase domain mutations in imatinib-naive patients: correlation with clonal cytogenetic evolution but not response to therapy. Blood 2005; 106: 2128–2137.

    Article  CAS  Google Scholar 

  7. Branford S, Rudzki Z, Parkinson I, Grigg A, Taylor K, Seymour JF et al. Real-time quantitative PCR analysis can be used as a primary screen to identify patients with CML treated with imatinib who have BCR-ABL kinase domain mutations. Blood 2004; 104: 2926–2932.

    Article  CAS  Google Scholar 

  8. Nardi V, Raz T, Cao X, Wu CJ, Stone RM, Cortes J et al. Quantitative monitoring by polymerase colony assay of known mutations resistant to ABL kinase inhibitors. Oncogene 2008; 27: 775–782.

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by NCI CA106796, Leukemia and Lymphoma Society Translational Research Grant and V Foundation for Cancer Research V Scholar Grant (VGO), NCI CA18029 (JPR) and the Canary Foundation (SA and FM).

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Oehler, V., Qin, J., Ramakrishnan, R. et al. Absolute quantitative detection of ABL tyrosine kinase domain point mutations in chronic myeloid leukemia using a novel nanofluidic platform and mutation-specific PCR. Leukemia 23, 396–399 (2009). https://doi.org/10.1038/leu.2008.183

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