FIGURE 2
FROM:
Src-family kinases play an essential role in differentiation signaling downstream of macrophage colony-stimulating factor receptors mediating persistent phosphorylation of phospholipase C-
2 and MAP kinases ERK1 and ERK2
C Bourgin-Hierle, S Gobert-Gosse, J Thérier, M-F Grasset and G Mouchiroud
BACK TO ARTICLEFigure 2.
PP2 and SU6656 inhibit macrophage differentiation of FD-Fms cells in response to M-CSF. (a) Effects of PP2 on FD-Fms cell morphology. Cells were cultivated for 3 days with interleukin-3 (IL-3) or with 2500 U ml-1 M-CSF in the presence or not of 10 
M PP2. (b) Flow cytometry analyses. Cells were cultivated for 3 days with IL-3 or with 2500 U ml-1 M-CSF in the presence or not of SFK inhibitors (10 M PP2 or 1 M SU6656). Undifferentiated cells were gated as indicated (low FSC/SSC) and the percentage of differentiated cells (that is, outside the gate) is shown in the upper right part of each dot-plot. (c) PP2 prevents induction of Mona expression in response to M-CSF. Cells were treated as above. For each time point, cell lysates were probed by immunoblotting with anti-Mona or anti-ERK1/2 antibody. ERK1/2 were used here as internal loading control.