Leukemia - Figure 1 for article: Src-family kinases play an essential role in differentiation signaling downstream of macrophage colony-stimulating factor receptors mediating persistent phosphorylation of phospholipase C-[gamma]2 and MAP kinases ERK1 and ERK2

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Src-family kinases play an essential role in differentiation signaling downstream of macrophage colony-stimulating factor receptors mediating persistent phosphorylation of phospholipase C-2 and MAP kinases ERK1 and ERK2

C Bourgin-Hierle, S Gobert-Gosse, J Thérier, M-F Grasset and G Mouchiroud

Figure 1.

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PP2 inhibits SFK activity, not M-CSFR phosphorylation. (a) Kinase activity was determined in SFK immunoprecipitates from lysates of FD-Fms cells stimulated with 2500 U ml^-1 of M-CSF with or without 10 M PP2, for times as indicated. Data are representative of two independent experiments. (b) Growth factor-deprived FD-Fms cells were treated or not for 30 min with 10 M PP2 then 2500 U ml^-1 M-CSF were added for times as indicated. Cell lysates were subjected to M-CSFR immunoprecipitation, followed by electrophoresis on a 7.5% SDS–PAGE, and then probed with anti-phosphotyrosine ( alpha PY) or anti-M-CSFR ( alpha M-CSFR) antibody.

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FIGURE 1

Src-family kinases play an essential role in differentiation signaling downstream of macrophage colony-stimulating factor receptors mediating persistent phosphorylation of phospholipase C-2 and MAP kinases ERK1 and ERK2

Figure 1.