TABLE OF CONTENTS

Volume 21, Issue 2 (February 2007)

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Editorial

The prognosis impact of BCR-ABL P-loop mutations: worse or not worse?

F E Nicolini, S Corm, Q-H L|[ecirc]|, C Roche-Lestienne and C Preudhomme

Leukemia 21: 193-194; doi:10.1038/sj.leu.2404490

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Review

Chromatin modifications and DNA double-strand breaks: the current state of play

T C Karagiannis and A El-Osta

Leukemia 21: 195-200; advance online publication, December 7, 2006; doi:10.1038/sj.leu.2404478

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Original Articles

Biomed-2

Improved reliability of lymphoma diagnostics via PCR-based clonality testing: |[mdash]| Report of the BIOMED-2 Concerted Action BHM4-CT98-3936

J H J M van Krieken, A W Langerak, E A Macintyre, M Kneba, E Hodges, R Garcia Sanz, G J Morgan, A Parreira, T J Molina, J Cabe|[ccedil]|adas, P Gaulard, B Jasani, J F Garcia, M Ott, M L Hannsmann, F Berger, M Hummel, F Davi, M Br|[uuml]|ggemann, F L Lavender, E Schuuring, P A S Evans, H White, G Salles, P J T A Groenen, P Gameiro, Ch Pott and J J M van Dongen

Leukemia 21: 201-206; advance online publication, December 14, 2006; doi:10.1038/sj.leu.2404467

Significantly improved PCR-based clonality testing in B-cell malignancies by use of multiple immunoglobulin gene targets. Report of the BIOMED-2 Concerted Action BHM4-CT98-3936

P A S Evans, Ch Pott, P J T A Groenen, G Salles, F Davi, F Berger, J F Garcia, J H J M van Krieken, S Pals, Ph Kluin, E Schuuring, M Spaargaren, E Boone, D Gonz|[aacute]|lez, B Martinez, R Villuendas, P Gameiro, T C Diss, K Mills, G J Morgan, G I Carter, B J Milner, D Pearson, M Hummel, W Jung, M Ott, D Canioni, K Beldjord, C Bastard, M H Delfau-Larue, J J M van Dongen, T J Molina and J Cabe|[ccedil]|adas

Leukemia 21: 207-214; advance online publication, December 14, 2006; doi:10.1038/sj.leu.2404479

Powerful strategy for polymerase chain reaction-based clonality assessment in T-cell malignancies Report of the BIOMED-2 Concerted Action BHM4 CT98-3936

M Br|[uuml]|ggemann, H White, P Gaulard, R Garcia-Sanz, P Gameiro, S Oeschger, B Jasani, M Ott, G Delsol, A Orfao, M Tiemann, H Herbst, A W Langerak, M Spaargaren, E Moreau, P J T A Groenen, C Sambade, L Foroni, G I Carter, M Hummel, C Bastard, F Davi, M-H Delfau-Larue, M Kneba, J J M van Dongen, K Beldjord and T J Molina

Leukemia 21: 215-221; advance online publication, December 14, 2006; doi:10.1038/sj.leu.2404481

Polymerase chain reaction-based clonality testing in tissue samples with reactive lymphoproliferations: usefulness and pitfalls. A report of the BIOMED-2 Concerted Action BMH4-CT98-3936

A W Langerak, T J Molina, F L Lavender, D Pearson, T Flohr, C Sambade, E Schuuring, T Al Saati, J J M van Dongen and J H J M van Krieken

Leukemia 21: 222-229; advance online publication, December 14, 2006; doi:10.1038/sj.leu.2404482

Human T-cell lines with well-defined T-cell receptor gene rearrangements as controls for the BIOMED-2 multiplex polymerase chain reaction tubes

Y Sandberg, B Verhaaf, E J van Gastel-Mol, I L M Wolvers-Tettero, J de Vos, R A F MacLeod, J G Noordzij, W A Dik, J J M van Dongen and A W Langerak

Leukemia 21: 230-237; advance online publication, December 14, 2006; doi:10.1038/sj.leu.2404486

Therapy

Age and high-dose methotrexate are associated to clinical acute encephalopathy in FRALLE 93 trial for acute lymphoblastic leukemia in children

M N Dufourg, J Landman-Parker, M F Auclerc, C Schmitt, Y Perel, G Michel, P Levy, G Couillault, V Gandemer, M D Tabone, F Demeocq, J P Vannier, T Leblanc, G Leverger and A Baruchel

Leukemia 21: 238-247; advance online publication, December 14, 2006; doi:10.1038/sj.leu.2404495

Acute Leukemias

The histone deacetylase inhibitor valproic acid potently augments gemtuzumab ozogamicin-induced apoptosis in acute myeloid leukemic cells FREE

B ten Cate, D F Samplonius, T Bijma, L F M H de Leij, W Helfrich and E Bremer

Leukemia 21: 248-252; advance online publication, November 23, 2006; doi:10.1038/sj.leu.2404477

In vivo apoptosis of CD8|[plus]| lymphocytes in acute myeloid leukemia patients: involvement of soluble HLA-I and Fas ligand

P Contini, M R Zocchi, I Pierri, A Albarello and A Poggi

Leukemia 21: 253-260; advance online publication, December 14, 2006; doi:10.1038/sj.leu.2404494

Reactive oxygen species mediate N-(4-hydroxyphenyl)retinamide-induced cell death in malignant T cells and are inhibited by the HTLV-I oncoprotein Tax FREE

N Darwiche, G Abou-Lteif and A Bazarbachi

Leukemia 21: 261-269; advance online publication, November 23, 2006; doi:10.1038/sj.leu.2404472

Risk stratification for survival and leukemic transformation in essential thrombocythemia: a single institutional study of 605 patients

N Gangat, A P Wolanskyj, R F McClure, C-Y Li, S Schwager, W Wu and A Tefferi

Leukemia 21: 270-276; advance online publication, December 14, 2006; doi:10.1038/sj.leu.2404500

Chronic Myeloproliferative Disorder

A unified definition of clinical resistance|[sol]|intolerance to hydroxyurea in essential thrombocythemia: results of a consensus process by an international working group

G Barosi, C Besses, G Birgegard, J Briere, F Cervantes, G Finazzi, H Gisslinger, M Griesshammer, L Gugliotta, C Harrison, H Hasselbalch, E Lengfelder, J T Reilly, J J Michiels and T Barbui

Leukemia 21: 277-280; advance online publication, November 16, 2006; doi:10.1038/sj.leu.2404473

Chronic Lymphocytic Leukemia

Regulation of the proapoptotic BH3-only protein BIM by glucocorticoids, survival signals and proteasome in chronic lymphocytic leukemia cells

D Iglesias-Serret, M de Frias, A F Santidri|[aacute]|n, L Coll-Mulet, A M Cosialls, M Barrag|[aacute]|n, A Domingo, J Gil and G Pons

Leukemia 21: 281-287; advance online publication, December 7, 2006; doi:10.1038/sj.leu.2404483

Cytogenetics and Molecular Genetics

Hypercalcemia in childhood acute lymphoblastic leukemia: frequent implication of parathyroid hormone-related peptide and E2A-HLF from translocation 17;19

T Inukai, K Hirose, T Inaba, H Kurosawa, A Hama, H Inada, M Chin, Y Nagatoshi, Y Ohtsuka, M Oda, H Goto, M Endo, A Morimoto, M Imaizumi, N Kawamura, Y Miyajima, M Ohtake, R Miyaji, M Saito, A Tawa, F Yanai, K Goi, S Nakazawa and K Sugita

Leukemia 21: 288-296; advance online publication, December 21, 2006; doi:10.1038/sj.leu.2404496

Stem Cells

Morphological and molecular characterization of novel population of CXCR4|[plus]| SSEA-4|[plus]| Oct-4|[plus]| very small embryonic-like cells purified from human cord blood |[ndash]| preliminary report

M Kucia, M Halasa, M Wysoczynski, M Baskiewicz-Masiuk, S Moldenhawer, E Zuba-Surma, R Czajka, W Wojakowski, B Machalinski and M Z Ratajczak

Leukemia 21: 297-303; advance online publication, November 30, 2006; doi:10.1038/sj.leu.2404470

Mesenchymal stem cells inhibit proliferation and apoptosis of tumor cells: impact on in vivo tumor growth

R Ramasamy, E W-F Lam, I Soeiro, V Tisato, D Bonnet and F Dazzi

Leukemia 21: 304-310; advance online publication, December 14, 2006; doi:10.1038/sj.leu.2404489

Normal Hemopoiesis

T-, B- and NK-lymphoid, but not myeloid cells arise from human CD34|[plus]|CD38|[minus]|CD7|[plus]| common lymphoid progenitors expressing lymphoid-specific genes

I Hoebeke, M De Smedt, F Stolz, K Pike-Overzet, F J T Staal, J Plum and G Leclercq

Leukemia 21: 311-319; advance online publication, December 14, 2006; doi:10.1038/sj.leu.2404488

Genomics

Folate metabolic gene polymorphisms and childhood acute lymphoblastic leukemia: a case|[ndash]|control study

A Gast, J L Bermejo, T Flohr, M Stanulla, B Burwinkel, M Schrappe, C R Bartram, K Hemminki and R Kumar

Leukemia 21: 320-325; advance online publication, November 30, 2006; doi:10.1038/sj.leu.2404474

Signal Transduction and Cytokines

Clinical and prognostic significance of cytokine receptor expression in adult acute lymphoblastic leukemia: interleukin-2 receptor |[alpha]|-chain predicts a poor prognosis

K Nakase, K Kita, H Miwa, K Nishii, M Shikami, I Tanaka, H Tsutani, T Ueda, K Nasu, T Kyo, H Dohy, H Shiku and N Katayama

Leukemia 21: 326-332; advance online publication, January 4, 2007; doi:10.1038/sj.leu.2404497

Lymphoma

Antiproliferative activity of RAD001 (everolimus) as a single agent and combined with other agents in mantle cell lymphoma

T Haritunians, A Mori, J O'Kelly, Q T Luong, F J Giles and H P Koeffler

Leukemia 21: 333-339; advance online publication, November 30, 2006; doi:10.1038/sj.leu.2404471

Myeloid sarcoma: clinico-pathologic, phenotypic and cytogenetic analysis of 92 adult patients FREE

S A Pileri, S Ascani, M C Cox, C Campidelli, F Bacci, M Piccioli, P P Piccaluga, C Agostinelli, S Asioli, D Novero, M Bisceglia, M Ponzoni, A Gentile, P Rinaldi, V Franco, D Vincelli, A Pileri Jr, R Gasbarra, B Falini, P L Zinzani and M Baccarani

Leukemia 21: 340-350; advance online publication, December 14, 2006; doi:10.1038/sj.leu.2404491

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Letters to the Editor

Further evidence for a pseudoautosomal gene for Hodgkin's lymphoma: Reply to |[lsquo]|The familial risk of Hodgkin's lymphoma ranks among the highest in the Swedish Family-Cancer Database|[rsquo]| by Altieri A and Hemminki K

M S Horwitz and M E Mealiffe

Leukemia 21: 351; advance online publication, December 7, 2006; doi:10.1038/sj.leu.2404487

High incidence of CALM-AF10 fusion and the identification of a novel fusion transcript in acute megakaryoblastic leukemia in children without Down's syndrome

M Abdelhaleem, K Beimnet, M Kirby-Allen, A Naqvi, J Hitzler and M Shago

Leukemia 21: 352-353; advance online publication, December 14, 2006; doi:10.1038/sj.leu.2404503

Acute myeloid leukemia cells constitutively express the immunoregulatory enzyme indoleamine 2,3-dioxygenase

A Curti, M Aluigi, S Pandolfi, E Ferri, A Isidori, V Salvestrini, I Durelli, A L Horenstein, F Fiore, M Massaia, M Piccioli, S A Pileri, E Zavatto, A D'Addio, M Baccarani and R M Lemoli

Leukemia 21: 353-355; advance online publication, December 14, 2006; doi:10.1038/sj.leu.2404485

CD56dimCD16|[plus]| NK cells downregulate CD16 following target cell induced activation of matrix metalloproteinases

B Grzywacz, N Kataria and M R Verneris

Leukemia 21: 356-359; doi:10.1038/sj.leu.2404499

Reply to Dr Grzywacz

O Penack and L Uharek

Leukemia 21: 359; doi:10.1038/sj.leu.2404498

Acute differentiated dendritic cell leukemia: a variant form of pediatric acute myeloid leukemia with MLL translocation

L Lo Nigro, L Sainati, A Leszl, E Mirabile, M Spinelli, C Consarino, A Di Cataldo, S Magro, C A Felix and G Basso

Leukemia 21: 360-362; advance online publication, January 4, 2007; doi:10.1038/sj.leu.2404480

The MTCP1 oncogene modifies T-cell homeostasis before leukemogenesis in transgenic mice

M Joiner, E Le Toriellec, G Despouy and M H Stern

Leukemia 21: 362-366; advance online publication, November 30, 2006; doi:10.1038/sj.leu.2404476

Different types of NPM1 mutations in children and adults: evidence for an effect of patient age on the prevalence of the TCTG-tandem duplication in NPM1-exon 12

C Thiede, E Creutzig, D Reinhardt, G Ehninger and U Creutzig

Leukemia 21: 366-367; advance online publication, December 7, 2006; doi:10.1038/sj.leu.2404519

JAK2 V617F mutation is a rare event in juvenile myelomonocytic leukemia

M Zecca, G Bergamaschi, C Kratz, E Bergstr|[auml]||[szlig]|er, C Danesino, P De Filippi, H Hasle, D Lisini, F Locatelli, A Pession, L Sainati, J Star|[yacute]|, M Trebo, M van den Heuvel-Eibrink, D W|[oacute]|jcik and C M Niemeyer on behalf of the European Working Group on MDS in childhood (EWOG-MDS)

Leukemia 21: 367-369; advance online publication, December 7, 2006; doi:10.1038/sj.leu.2404484

Mitochondrial DNA minisatellites as new markers for the quantitative determination of hematopoietic chimerism after allogeneic stem cell transplantation

M-G Shin, H-J Kim, H-R Kim, Il-K Lee, H Kook, D Cho, S-J Kee, J-H Shin, S-P Suh and D-W Ryang

Leukemia 21: 369-373; advance online publication, December 15, 2006; doi:10.1038/sj.leu.2404502

Recurrence of clonal hematopoiesis after discontinuing pegylated recombinant interferon-|[alpha]| 2a in a patient with polycythemia vera

T Ishii, M Xu, Y Zhao, W-Y Hu, S Ciurea, E Bruno and R Hoffman

Leukemia 21: 373-374; advance online publication, November 23, 2006; doi:10.1038/sj.leu.2404475

Epigenetic regulation of brain expressed X-linked-2, a marker for acute myeloid leukemia with mixed lineage leukemia rearrangements

C Fischer, H G Drexler, J Reinhardt, M Zaborski and H Quentmeier

Leukemia 21: 374-377; doi:10.1038/sj.leu.2404493

Chronic myeloproliferative disorders: the bone marrow stromal component is not involved in the malignant clone

M Di Ianni, L Moretti, B Del Papa, M De Ioanni, E Bonifacio, F Falzetti and A Tabilio

Leukemia 21: 377-378; doi:10.1038/sj.leu.2404505

Aberrant expression of HLA-DR antigen by bone marrow-derived mesenchymal stromal cells from patients affected by acute lymphoproliferative disorders

F Lanza, D Campioni, S Moretti, L Ferrari, R Rizzo, R Baricordi and A Cuneo

Leukemia 21: 378-381; advance online publication, December 14, 2006; doi:10.1038/sj.leu.2404492

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