Leukemia

FIGURE 2

FROM:

TEL-Syk fusion constitutively activates PI3-K/Akt, MAPK and JAK2-independent STAT5 signal pathways

T Kanie, A Abe, T Matsuda, Y Kuno, M Towatari, T Yamamoto, H Saito, N Emi and T Naoe

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Figure 2.

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TEL-Syk fusion proteins were associated with PI3-K and constitutively activated Akt. (a) BaF3 cells were deprived of IL-3 for 12 h and then restimulated with 5 ng/ml of IL-3 for 30 min. Cell lysates were immunoblotted with antibody against phospho-Akt and reprobed with antibody against Akt. Akt was activated in TEL-Syk-transformed BaF3 cells in the absence of IL-3. (b) Cell lysates were immunoprecipitated with antibody against the 85K subunit of PI3-K and immunoblotted with human-specific Syk mAb. The same membrane was stripped and reprobed with antibody against the 85K subunit of PI3-K. TEL-Syk fusion products (lane 2) were co-precipitated with 85K subunit of PI3-K, and BaF3 cell lysates (lane 1) and TELDeltaPNT-Syk lysates (lane 3) were not. Lane 4 consisted of whole-cell lysates of TEL-Syk and lane 5 consisted of TELDeltaPNT-Syk.

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