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PH-Positive ALL and Lineage Involvement

The existence of lymphoid lineage restricted Philadelphia chromosome-positive acute lymphoblastic leukemia with heterogeneous bcr-abl rearrangement

Leukemia volume 14, pages 1122–1126 (2000)Cite this article

Abstract

Analysis of lineage involvement was performed in 17 Philadelphia chromosome-positive acute lymphoblastic leukemia patients with no history of chronic myeloproliferative disorder. The percentage of blastic cells as defined by flow cytometry matched that of the Ph-positive cells in 14 out of 17 patients. The bcr-abl rearrangement was investigated by fluorescent in situhybridization in morphologically identified blastic cells, myeloid elements, lymphocytes and erythroblasts using a combined light and fluorescent microscopical imaging. Lymphoid lineage restriction could be determined in all but three of the patients. These 14 patients exhibited heterogeneity in terms of m-bcr and M-bcr types of translocation as revealed by reverse transcription polymerase chain reaction. The three patients with multilineage involvement and M-bcr type of translocation reverted to chronic phase and the percentage of Ph-positive cells remained high. Thus, we could identify an uncommitted stem cell origin among Ph-positive ALLs only in those patients whose disease subsequently proved to be a lymphoid blastic crisis with clinically silent chronic phase.

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Acknowledgements

This work was supported by grants 051/POT/97 01 sponsored by the Hungarian Scientific Council of Health (ETT) and T 025812 sponsored by the Hungarian Scientific Research Fund (OTKA).

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Authors and Affiliations

  1. Department of Pathology, University Medical School of Pécs, Hungary

    L Pajor, JA Vass, L Kereskai & P Jáksó

  2. Department of Pediatrics, University Medical School of Pécs, Hungary

    P Kajtár

  3. Department of 1st Internal Medicine, University Medical School of Pécs, Hungary

    Á Szomor

  4. Department of Internal Medicine, Kaposi Mór Hospital, Kaposvár, Hungary

    M Egyed

  5. Department of Hematology, Markusovszky Hospital, Szombathely, Hungary

    J Iványi

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  1. L Pajor
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Pajor, L., Vass, J., Kereskai, L. et al. The existence of lymphoid lineage restricted Philadelphia chromosome-positive acute lymphoblastic leukemia with heterogeneous bcr-abl rearrangement. Leukemia 14, 1122–1126 (2000). https://doi.org/10.1038/sj.leu.2401794

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