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Hairy Cell Leukemia

P53 mutations in hairy cell leukemia

Leukemia volume 14, pages 706–711 (2000)Cite this article

Abstract

We have studied the frequency of p53 mutations in genomic DNA extracted from peripheral blood or the spleen of 61 patients with hairy cell leukemia using PCR-SSCP and automated cycle sequencing. We identified exon 5–8 mutations in 17 cases, corresponding to a frequency of 28%. In four cases, mutations were localized in exon 5; one patient with atypical HCL had a mutation in exon 6 at the 3′ boundary; five cases showed mutations in exon 7, while exon 8 was found to be mutated in seven cases. The mutations found could be divided into three major categories: structural (n = 9), inactivating (n = 6), and neutral (n = 2) mutations. None of the three transitions found occurred at CpG dinucleotides. The rate of p53 mutations found in this large cohort of HCL patients is unexpectedly high as in other non-Hodgkin lymphomas p53 mutations predict for poor treatment outcome. The character of the mutations we have found is entirely different from that described in other hematologic malignancies.

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Acknowledgements

Dr J Demeter is a scientific fellow of the Alexander von Humboldt Foundation, Bonn, Germany.

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Authors and Affiliations

  1. Department of Molecular Genetics, Semmelweis University, School of Medicine, Budapest, Hungary

    EA König, R de Châtel & ZL Marcsek

  2. Centre for Environmental Health, University of Victoria, Victoria BC, Canada

    EA König, WC Kusser, C Day & BW Glickman

  3. First Department of Medicine, Semmelweis University Medical School, Hungary

    R de Châtel, ZL Marcsek & J Demeter

  4. Department of Internal Medicine III, University of Ulm, Germany

    F Porzsolt, G Messer, M Schmid & J Demeter

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  1. EA König
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König, E., Kusser, W., Day, C. et al. P53 mutations in hairy cell leukemia. Leukemia 14, 706–711 (2000). https://doi.org/10.1038/sj.leu.2401721

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