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| May 1999, Volume 13, Number 5, Pages 799-807 |
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| Original manuscript: lymphoma |
| Gastric low-grade MALT lymphoma, high-grade MALT lymphoma and diffuse large B cell lymphoma show different frequencies of trisomy |
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| M A Hoeve1,a, I A M Gisbertz2, H C Schouten3, E Schuuring1, F J Bot2, J Hermans4, A Hopman5, PhM Kluin1, J-W Arends2 and J H J M van Krieken1 |
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1Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands
2Department of Pathology, University Hospital Maastricht, Maastricht, The Netherlands
3Department of Internal Medicine, University Hospital Maastricht, Maastricht, The Netherlands
4Department of Medical Statistics, Leiden University Medical Center, Leiden, The Netherlands
5Department of Molecular Cellbiology and Genetics, University Hospital Maastricht, Maastricht, The Netherlands
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aCorrespondence: MA Hoeve, Department of Pathology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands; Fax: 31 71 5248158 MA Hoeve and IAM Gisbertz contributed equally to this work |
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| Abstract |
| Gastric MALT lymphoma is a distinct entity related to Helicobacter pylori gastritis. Some studies suggest a role for trisomy 3 in the genesis of these lymphomas, but they mainly focused on low-grade MALT lymphoma. Gastric MALT lymphoma, however, comprises a spectrum from low- to high-grade cases. Furthermore, its exact relation to primary diffuse large B cell lymphoma (DLBCL) of the stomach is not clear. We applied in situ hybridisation (ISH) with centromeric probes on 43 samples of 39 patients with primary gastric lymphoma (13 samples with low-grade MALT lymphoma, 25 with high-grade MALT lymphoma and five with DLBCL) to detect numerical aberrations of 10 chromosomes. ISH was performed immunohistochemically on nuclei isolated from paraffin-embedded resection tissue and on whole paraffin sections using immunofluorescence. In six of 13 low-grade MALT lymphomas trisomy was detected (46%) and mostly involved chromosome 3 (33%). In high-grade MALT lymphomas, trisomies were found in 16 of 25 cases (64%), mainly involving chromosomes 12 and 18. Trisomy 3 was present in only 13% of these cases. Of five DLBCL, only one showed trisomy. Nine of the 16 aberrant high-grade MALT lymphomas (56%) showed trisomy of more than one chromosome per case vs two of six for low-grade cases. In lymphomas with separate low- and high-grade tumour components some trisomies were detected in both components, whereas others occurred only in the high-grade tumour cells. This supports the hypothesis that high-grade MALT lymphomas can develop from a low-grade type and that this progression is accompanied by the acquisition of more genetic aberrations. However, trisomy 3 probably does not play a major role in this progression. |
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| Keywords |
| gastric lymphoma; MALT lymphoma; in situ hybridisation; trisomy 3; numerical aberration |
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| Received 23 November 1998; accepted 3 February 1999 |
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| May 1999, Volume 13, Number 5, Pages 799-807 |
| Table of contents Previous Abstract Next Article PDF |
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