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| Biotechnical methods section (BTS) |
| Fluorescent BAT-25 and BAT-26 analysis of T cell prolymphocytic leukaemia |
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| P S Bradshaw1, R Hamoudi2, T Min1, D Catovsky1, R S Houlston3 and M R Yuille1 |
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1Academic Department of Haematology and Cytogenetics, Institute of Cancer Research, Sutton, UK
2Cancer Gene Cloning Centre, Institute of Cancer Research, Sutton, UK
3Section of Cancer Genetics, Institute of Cancer Research, Sutton, UK
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Correspondence to: M R Yuille, Academic Department of Haematology and Cytogenetics, Institute of Cancer Research, Sutton, Surrey, SM2 5NG, UK
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| Abstract |
| T cell prolymphocytic leukaemia (T-PLL) is a chronic mature T cell malignancy with many random cytogenetic abnormalities. These imply that maintenance of genomic integrity is impaired. This is supported by the recent finding that the ataxia telangiectasia gene, ATM, which contributes to maintaining genomic integrity, is frequently mutated in this disease. To evaluate in T-PLL the role of other genes with comparable function, a fluorescence-based semi-automated assay was developed for BAT-25 and BAT-26. These markers contain sequences that are particularly unstable in cells with DNA mismatch repair defects. Application of the assay to 20 T-PLL cases found no evidence for such defects. |
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| Keywords |
| T cell prolymphocytic leukaemia; microsatellite instability; BAT-25; BAT-26 |
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| Received 3 June 1999; accepted 10 August 1999 |
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| December 1999, Volume 13, Number 12, Pages 2104-2106 |
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