TABLE 1

FROM:

Reduced acetaminophen-induced liver injury in mice by genetic disruption of IL-1 receptor antagonist

Takuya Ishibe, Akihiko Kimura, Yuko Ishida, Tatsunori Takayasu, Takahito Hayashi, Koichi Tsuneyama, Kouji Matsushima, Ikuhiro Sakata, Naofumi Mukaida and Toshikazu Kondo

Table 1. Sequences of the primers used for RT–PCR

Transcript	Sequence	Annealing temperature (°C)	Cycle	Product size (bp)
IL-1	(F) 5'-TGGCCAAAGTTCCTGACTTGTTTG-3'	60	32	488
	(R) 5'-CAGGCTATTTAACCAAGTGGTGCT-3'
IL-1	(F) 5'-CAAGGCCACAGGTATTTTGT-3'	56	28	504
	(R) 5'-GAAATGCCACCTTTTGACAG-3'
CXCL1/KC	(F) 5'-GGATTCACCTCAAGAACATCCAGAG-3'	62	28	454
	(R) 5'-CACCCTTCTACTAGCACAGTGGTTG-3'
CXCL2/MIP-2	(F) 5'-GAACAAAGGCAAGGCTAACTGA-3'	58	34	204
	(R) 5'-AACATAACAACATCTGGGCAAT-3'
CCL2/MCP-1	(F) 5'-ACTGAAGCCAGCTCTCTCTTCCTC-3'	63	30	274
	(R) 5'-TTCCTTCTTGGGGTCAGCACAGAC-3'
CCL3/MIP-1	(F) 5'-GCCCTTGCTGTTCTTCTCTGT-3'	60	32	258
	(R) 5'-GGCAATCAGTTCCAGGTCAGT-3'
CYP1A2	(F) 5'- AGAGCGGTTTCTTACCAATA-3'	60	30	526
	(R) 5'-GCCAGAGTAGGCAAATCT-3'
CYP2E1	(F) 5'- GGATGAATATGCCCTACATG-3'	60	32	468
	(R) 5'- TGATGGGCAGCAGGTCTCAT-3'
CYP3A11	(F) 5'-TGAGGCAGAAGGCAAAGAAA-3'	58	32	593
	(R) 5'-CAGGTATTCCATCTCCATCACA-3'
-Actin	(F) 5'-TTCTACAATGAGCTGCGTGTGGC-3'	62	26	456
	(R) 5'-CTCATAGCTCTTCTCCAGGGAGGA-3'

 bp, base pair; F, forward primer; R, reverse primer; IL, interleukin.