Laboratory Investigation

FIGURE 2

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Differential transplantability of human endothelial cells in colorectal cancer and renal cell carcinoma primary xenografts

Laura Sanz, Ángel M Cuesta, Clara Salas, César Corbacho, Carmen Bellas and Luís Álvarez-Vallina

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Figure 2.

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Immunohistochemical analysis of preimplantation (day 0) CRC tissue sections and xenografts resected 10 and 30 days after implantation using antihuman CD34 (a, c, e and g) and antimouse CD34 (b, d, f and h). Original magnification, times 400. (i) Temporal changes in vessel density (meanplusminuss.d. of CD34-positive vessels in five high-power fields per section). Quantification of vasculature in CRC initial specimens and xenografts harvested 10, 20 or 30 days after implantation. (j) Double immunostaining (detail) of mouse CD34 (DAB) and human laminin (permanent red) of xenograft sections 10 days postimplantation. Solid arrows indicate murine ECs (reddish brown), and arrowheads the laminin-rich basement membrane (pink). Original magnification, times 1000, immersion oil.

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