FIGURE 3

Localization of MCMV IE1-positive cells in the labyrinths in the MCMV-i.p.-infected mice with i.c. injections of LPS. Newborn mice were i.p. infected with MCMV and received i.c. injection of LPS four times. Mice were killed 6 days after infection, and serial sections of the inner ears were made after fixation and decalcification. Sections were reacted with anti-IE1 mAb, incubated with horseradish peroxidase-labeled secondary Ab, and colored with DAB. (a) Low-power view of the labyrinth, HE stain. CN, cochlear nerve; M, mesenchymal region; SG, spiral ganglia; ST, scala tympani; SV, scala vestibuli; SM, scala media; CO, Corti organ. (b) IE1-positive cells in the adjacent section. IE1-positive cells were observed in the following higher power views: (c) in the mesenchymal region, (d) along the cochlear nerve (arrows), (e) in the epithelial cells of ST, (f) in the spiral limbus (SL), (g) in the cerebral ventricular wall. VZ, ventricular zone; SVZ, subventricular zone and (h) in the cerebral meninges (Men).