FIGURE 2

Mkk3-/- mice are resistant to hyperglycaemia in MLD-STZ. (a) Fasting blood glucose was measured on a weekly basis after MLD-STZ administration. Most WT mice developed hyperglycaemia (>7 mmol/l) by week 3. In contrast, fasting blood glucose remained normal in Mkk3-/- mice after MLD-STZ administration (^*** P<0.0001 vs WT). (b) The insulin content of whole pancreas tissue was measured in normal mice (open bars) and mice at week 4 of MLD-STZ (closed bar) (n=4). (c) The degree of -cell granulation in pancreatic islets was assessed by Gomori's aldehyde fuchsin staining in normal mice (open bars) and at week 4 of MLD-STZ (closed bars) in WT and Mkk3-/- mice (n=7–8). Examples of Fuchsin staining in: (d) normal WT mice; (e) MLD-STZ in WT mice showing a dramatic reduction in -cell granulation, and; (f) MLD-STZ in Mkk3-/- mice which are largely protected from loss of -cell granulation.