Laboratory Investigation

FIGURE 4

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Coexpression of myofibroblast and macrophage markers: novel evidence for an in vivo plasticity of chorioamniotic mesodermal cells of the human placenta

Sung-Su Kim, Roberto Romero, Jung-Sun Kim, Asad Abbas, Jimmy Espinoza, Juan Pedro Kusanovic, Sonia Hassan, Bo Hyun Yoon and Chong Jai Kim

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Figure 4.

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Chorionic mesodermal cells and the regulation of CD14 mRNA expression by PU.1. The cells were obtained by sequential treatment of chorion-decidua with dispase II and collagenase. (a) CD14 mRNA expression is increased in the chorionic mesodermal cells following transient transfection with full-length human PU.1 cDNA. CD14 mRNA expression was evaluated using mesodermal cells transfected with PU.1 expression vector at various concentrations for 24 h by qRT-PCR using exon-specific primers and normalization to beta-actin mRNA. (b) Immunoblotting of PU.1-transfected cells showing increased CD14 expression following PU.1 expression.

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