FIGURE 1
FROM:
Normal human primary CD4+ T lymphocytes synthesize and release functional osteoprotegerin in vitro
Arpita Chakravarti, Andrée-Anne Marceau, Louis Flamand and Patrice E Poubelle
BACK TO ARTICLEFigure 1.
Production of OPG by normal human nonadherent and adherent PBML. (a) Peripheral blood mononuclear leukocytes (PBML), nonadherent and adherent fractions of these PBML were incubated at 5 
106/ml in RPMI 1640+10% FBS for 9 days; supernatants were successively collected to evaluate the accumulation of OPG by EIA. Results are expressed as means
s.e.m. (PBML: n=6, nonadherent PBML: n=15, adherent PBML: n=5). (b) Nonadherent PBML (5 106/ml) were incubated in RPMI 1640+10% FBS at 37°C for 9 days in the presence of 10 
g/ml anti-CD3 mAb coated to plastic. Results are expressed as meanss.e.m. (n=5). Statistics: paired two-tailed t-test, *
P<0.05.
