Laboratory Investigation

FIGURE 2

FROM:

ROCK-II mediates colon cancer invasion via regulation of MMP-2 and MMP-13 at the site of invadopodia as revealed by multiphoton imaging

Ramana Vishnubhotla, Shan Sun, Jameela Huq, Marinka Bulic, Anil Ramesh, Grace Guzman, Michael Cho and Sarah C Glover

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Figure 2.

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ROCK-II localizes to the actin cytoskeleton in non-malignant intestinal cell lines, but localizes at the cell periphery in malignant cell lines. Non-malignant (NCM 460 (a) and IEC-6 (b)) and malignant intestinal cell lines (Caco-2 E (c), SW620 (d), and HCT-116 (e)) were seeded onto type I collagen scaffolds and allowed to invade over a 24-h period. Scaffolds were then fixed and stained for ROCK II (green), actin (red), and nuclei (blue). Non-malignant cell lines show ROCK-II colocalizing with the actin cytoskeleton. Malignant cell lines show heavy ROCK-II expression localizes to the cell periphery. Negative controls for each cell line were probed with a goat IgG and no staining was seen (data not shown). Bar=50 mum.

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