Laboratory Investigation

FIGURE 5

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Fluorescent microangiography (FMA): an improved tool to visualize the pulmonary microvasculature

Andre E Dutly, Lakshmi Kugathasan, Judy E Trogadis, Shaf H Keshavjee, Duncan J Stewart and David W Courtman

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Figure 5.

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Combined Angiography and Immunostaining. Microsphere perfusion (green) was combined with thick section immunostaining for either smooth muscle alpha-actin or laminin (both red). Smooth muscle staining (a) revealed the extent of muscularization in the normal rat lung. Laminin staining (b) displayed the perfusion profile in the alveolar septi (blue is nuclear counterstaining). Both images are projections of a confocal z-series taken through a 200 mum section. Scale bars=100 mum.

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