Laboratory Investigation

FIGURE 7

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Biobanking of fresh frozen tissue: RNA is stable in nonfixed surgical specimens

Patrick Micke, Mitsuhiro Ohshima, Simin Tahmasebpoor, Zhi-Ping Ren, Arne Östman, Fredrik Pontén and Johan Botling

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Figure 7.

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Gene expression levels under different storage conditions. RNA isolated from the tonsil tissue experiment was transcribed to cDNA and analysed using primers for cfos, TGFbeta1, SMAD7, HIF1alpha, Bcl2 and PCNA. The results were normalised to GAPDH expression. Relative gene expression at three different time points (0.5, 6 and 16 h) was compared to time point 0 h (set to 1.0). The results are shown as mean and standard deviation.

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