Laboratory Investigation

FIGURE 4

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Biobanking of fresh frozen tissue: RNA is stable in nonfixed surgical specimens

Patrick Micke, Mitsuhiro Ohshima, Simin Tahmasebpoor, Zhi-Ping Ren, Arne Östman, Fredrik Pontén and Johan Botling

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Figure 4.

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Morphology of tonsil tissue under different transport conditions. Frozen tonsil specimens were cut in sections (4 mum) by the tape transfer technique and stained with hematoxylin–eosin. The panels show the histology of a reference sample that was frozen immediately after surgery (a) and sections from samples stored for 16 h at room temperature (b), on ice (c), in normal saline (d) and in RNAlater (e). Original magnification times 10.

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