Laboratory Investigation

FIGURE 2

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Biobanking of fresh frozen tissue: RNA is stable in nonfixed surgical specimens

Patrick Micke, Mitsuhiro Ohshima, Simin Tahmasebpoor, Zhi-Ping Ren, Arne Östman, Fredrik Pontén and Johan Botling

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Figure 2.

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RNA integrity of tonsil tissue under different transport conditions. Purified RNA was analysed using microchip gel electrophoresis (Bioanalyzer) and the results are presented as electropherograms. The RNA of all samples (stored at room temperature (RT), on ice, in normal saline or RNAlater) at all time points (0, 0.5, 1, 3, 6 and 16 h) showed distinct ribosomal peaks indicating no significant degradation. Representative RNA samples purified with a Qiagen column are shown.

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