TABLE 1
FROM:
Notch2-positive progenitors with the intrinsic ability to give rise to pancreatic ductal cells
Kwang M Lee, Hiroaki Yasuda, Michael A Hollingsworth and Michel M Ouellette
BACK TO ARTICLETable 1. Sequences of PCR primers used and size of expected fragments
| Target mRNA |
Primer sequence (5'  3')
| Accession number | Size |
|---|---|---|---|
 -Actin | CGGGACCTGACTGACTACCT | BC001301 | 351 |
| CAGCACTGTGTTGGCGTACA | |||
| Carbonic anhydrase II | AAGGAACCCATCAGCGTCAG | NM_000067 | 357 |
| AAAGCACCAACCAGCCACAG | |||
| Cytokeratin 7 | TGAGATCGACAACATCAAGAAC | NM_005556 | 414 |
| CGGATGGAATAAGCCTTCAG | |||
| Cytokeratin 8 | ACCGACGAGATCAACTTCC | M34225 | 160 |
| CAATATCCTCGTACTGTGCC | |||
| Cytokeratin 19 | GCCACTACTACACGACCATCC | NM_002276 | 334 |
| GAATCCACCTCCACACTGACC | |||
| Desmin | GCTACTCTAGCTCGCATTGAC | U59167 | 403 |
| ATTCCCGCATCTGCCTCATC | |||
| GAPDH | ATCTTCTTTTGCGTCGCC | NM_002046 | 250 |
| GATGACAAGCTTCCCGTTC | |||
| GFAP | ACATCGAGATCGCCACCTAC | J04569 | 219 |
| ACATCACATCCTTGTGCTCC | |||
| Glucagon | ATCTGGACTCCAGGCGTGCC | BC005278 | 179 |
| AGCAATGGATTCCTTGGCAG | |||
| HES1 | AGCACAGAAAGTCATCAAAGCC | AF264785 | 259 |
| TTCATGCACTCGCTGAAGCC | |||
| Insulin | CAGCCGCAGCCTTTGTGAAC | NM_000207 | 170 |
| CAGGCTGCCTGCACCAGGG | |||
| Jagged1 | TTTTTTTTTCTCCCCTCCCTC | AF003837 | 230 |
| CCACTTTTCAAAAGCCCTTTC | |||
| IDR-1 | TCCTGGAGCGGTTCTACGAC | NM_000927 | 408 |
| GGGCTTCTTGGACAACCTTTTCA | |||
| Nestin | CGTTGGAACAGAGGTTGGAG | X65964 | 262 |
| TCCTGAAAGCTGAGGGAAG | |||
| Notch2 | ACTTCCTGCCAAGCATTCC | NM_024408 | 346 |
| GTCCATGTCTTCAGTGAGAAC | |||
| PECAM-1 | TCAAGAAAAGCAACACAGTCC | M28526 | 309 |
| TCACCTTCACCCTCAGAACC | |||
| Somatostatin | CGTCAGTTTCTGCAGAAGTCC | NM_001048 | 196 |
| CCATAGCCGGGTTTGAGTTA | |||
| Thrombomodulin | GGTGATTAGAGGGAGGAGAATG | M16552 | 402 |
| GAAGAATGGGGAATAAGTGGG | |||
| VE-cadherin | CAACTTCCCCTTCTTCACCC | U84722 | 460 |
| TCCAATGCTATGCCTAGCCG |
GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GFAP: glial fibrillary acidic protein; PECAM-1: platelet endothelial cell adhesion molecule-1; HES1: hairy/enhancer of split 1; MDR: multidrug resistance. For each primer pair used, the sequence of the forward (top) and reverse (bottom) primers is given with the accession number of the genebank entry from which they were derived. The right column indicates the size of the expected PCR products (in base pairs).
