FIGURE 4
FROM:
Lebectin, a novel C-type lectin from Macrovipera lebetina venom, inhibits integrin-mediated adhesion, migration and invasion of human tumour cells
Sameh Sarray, Virginie Berthet, Juan J Calvete, Jeannine Secchi, Jacques Marvaldi, Mohamed El Ayeb, Naziha Marrakchi and José Luis
BACK TO ARTICLEFigure 4.
Lebectin inhibits the adhesion of various tumour cells. IGR39 (a), HT1080 (c) or HT29-D4 (d) cells were preincubated with 25 
g/ml lebectin for 30 min at room temperature. Cells were then added to 96-well microtiter plates coated with 10 g/ml fibrinogen (Fg), fibronectin (Fn), vitronectin (Vn), type I or type IV collagens (Coll I and Coll IV, respectively) or poly-L-lysine (PL) or with 2 g/ml laminin (Ln) and allowed to adhere for 1 h or 2 h at 37°C. After washing, adherent cells were stained with crystal violet, solubilized by SDS and absorbance was measured at 600 nm. (b) HT1080 (-
-) or K562 (-
-) cells were preincubated with the indicated concentrations of lebectin and allowed to adhere to microtiter plates coated with 10 g/ml fibrinogen (HT1080 cells) or fibronectin (K562 cells). Data shown are means (
s.d.) from two to four experiments performed in triplicate. They are expressed as a percentage of adhesion in the absence of lebectin.
