Laboratory Investigation

FIGURE 2

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Infiltration of CD8+ T cells containing RANTES/CCL5+ cytoplasmic granules in actively inflammatory lesions of human chronic gastritis

Noriko Ohtani, Haruo Ohtani, Takashi Nakayama, Hiroshi Naganuma, Eiichi Sato, Toshio Imai, Hiroshi Nagura and Osamu Yoshie

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Figure 2.

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Immunohistochemistry for chemokine receptors. Low-power views of CCR5 (a), CXCR3 (b), and CCR4 (c), showing representative staining patterns (brown color). Hematoxylin counterstaining (a–c). Double staining for CCR5 and CD4 (f), and CCR5 and CD8 (g) (CCR5 in red, and CD4 or CD8 in blue). Representative results of three patients are shown. Localization of CX3CR1+ cells (brown color) around the neck region (f), with red and green asterisks indicating the pit and gastric gland areas, respectively. Negative control with mouse IgG1 (g). Negative controls with mouse IgG2a, IgG2b, and normal rabbit serum also showed no specific reactivity (not shown). Scale bars, 50 mum in a–c, f, and g, and 20 mum in d and e.

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