Laboratory Investigation

FIGURE 2

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Inhibition of transforming growth factor bold italic beta decreases pancreatic fibrosis and protects the pancreas against chronic injury in mice

Yoshikuni Nagashio, Hikaru Ueno, Michio Imamura, Hiroshi Asaumi, Shiro Watanabe, Taizo Yamaguchi, Masashi Taguchi, Mitsuo Tashiro and Makoto Otsuki

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Figure 2.

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Effects of adenovirus-mediated gene transfer (AdTbeta-ExR) on histological changes. Mice were injected once intraperitoneally with either AdLacZ (a, c, e, and g) or AdTbeta-ExR (b, d, f, and h), or with saline (not shown), and then treated with cerulein for 3 weeks. Pancreatic sections were histologically examined by Azan staining (a and b) or by immunostaining with antibodies against alpha-SMA (c and d), collagen type I (e and f), or fibronectin (g and h). In mice treated with AdTbeta-ExR, alpha-SMA-positive cells, collagen type I, and fibronectin ware clearly reduced compared with those in AdLacZ-treated mice. Original magnification, times 200.

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