Laboratory Investigation

FIGURE 5

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Human neutrophils produce interferon gamma upon stimulation by interleukin-12

Frédéric Ethuin, Bénédicte Gérard, Jamel E Benna, Anne Boutten, Marie-Anne Gougereot-Pocidalo, Laurent Jacob and Sylvie Chollet-Martin

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Figure 5.

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Western blot analysis of IFNitalic gamma expression by human PMN. Total homogenates of resting purified PMN or LPS+IL-12-treated PMN were added to 2 times Laemmli sample buffer, and proteins were resolved by SDS-PAGE. Proteins were immunoblotted after transfer to nitrocellulose membranes. Recombinant human IFNitalic gamma (rh IFNitalic gamma) served as positive control.

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