Laboratory Investigation

FIGURE 4

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Human neutrophils produce interferon gamma upon stimulation by interleukin-12

Frédéric Ethuin, Bénédicte Gérard, Jamel E Benna, Anne Boutten, Marie-Anne Gougereot-Pocidalo, Laurent Jacob and Sylvie Chollet-Martin

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Figure 4.

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RT-PCR analysis of IFNitalic gamma mRNA expression in PMN. Total RNA was extracted from resting (PMN-) and activated PMN (LPS+IL-12+IL-15) (PMN+). After reverse transcription, PCR was carried out with specific pairs of primers designed for IFNitalic gamma and beta-actin. Total lung tissue served as positive control (+). The figure indicates the size of amplification products relative to molecular weight standards run in parallel (MW) and the negative control (-) (PCR buffer without cDNA).

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