Laboratory Investigation

FIGURE 1

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Functional Expression of Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand in Human Colonic Adenocarcinoma Cells

Hidekazu Inoue, Katsuya Shiraki, Takenari Yamanaka, Shigeru Ohmori, Takahisa Sakai, Masatoshi Deguchi, Hiroshi Okano, Kazumoto Murata, Kazushi Sugimoto and Takeshi Nakano

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Figure 1.

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a, Expression of TNF-related apoptosis-inducing ligand (TRAIL-R1) (56 kDa) and TRAIL-R2 (48 kDa) in human colonic adenocarcinoma cell lines. Cellular lysates were separated by SDS-PAGE and transferred to nitrocellulose. TRAIL-R1 and TRAIL-R2 levels were detected by Western blotting. b, Detection of TRAIL-R3 and TRAIL-R4 mRNA in human colonic adenocarcinoma cell lines by RT-PCR. A 612-bp human TRAIL-R3-specific sequence, a 453-bp TRAIL-R4-specific sequence, and an 838-bp beta-actin sequence were amplified from total RNA isolated from human colonic adenocarcinoma cell lines, separated by agarose gel electrophoresis, and visualized by ethidium bromide staining.

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