1. ¹Department of Internal Medicine I, University of Regensburg, Regensburg, Germany
2. ²Institute of Pathology, University of Regensburg, Regensburg, Germany
3. ³Sidney Kimmel Cancer Center, San Diego, California
4. ⁴Institute of Pathology, Kassel, Germany

Correspondence: Dr. Frank Kullmann, Department of Internal Medicine I, University of Regensburg, D-93042 Regensburg, Germany. E-mail: frank.kullmann@klinik.uni-regensburg.de

Received 18 May 2001.

Abstract

Because colorectal epithelia are prone to malignant transformation, it is important to understand their normal regulation and then to identify differences between the normal cells and the transformed cells. We investigated the gene expression pattern along colonic crypts using a novel gene expression analysis strategy. We combined laser-mediated microdissection of distinct areas within colonic crypts and used modified RNA arbitrarily primed PCR to generate probes for cDNA array hybridization. In the basal part of the crypt, proliferation-related and cell cycle-related genes such as the multifunctional transcription factor e2f-1 or the mismatch-related gene p58/HHR23B were predominantly expressed. In the lumenal part of the crypt, up-regulations of the cysteine protease mch4 and the proto-oncogene c-jun were found. Our findings indicate that e2f1, p58/HHR23B, and mch4 may be involved in key mechanisms leading to malignant transformation in the colonic crypt. Our results also suggest that the technique elucidated here allows identification of gene expression patterns in distinct areas of intestinal tissue samples.